Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   CEW83_RS10645 Genome accession   NZ_CP022187
Coordinates   2341546..2342397 (+) Length   283 a.a.
NCBI ID   WP_108949320.1    Uniprot ID   A0A2U8GQ29
Organism   Parazoarcus communis strain TSPY31     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2336546..2347397
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CEW83_RS10630 (CEW83_10620) - 2337185..2338378 (+) 1194 WP_234418772.1 HlyC/CorC family transporter -
  CEW83_RS10635 (CEW83_10625) pilB 2338518..2340230 (+) 1713 WP_108949318.1 type IV-A pilus assembly ATPase PilB Machinery gene
  CEW83_RS10640 (CEW83_10630) pilC 2340256..2341494 (+) 1239 WP_108949319.1 type II secretion system F family protein Machinery gene
  CEW83_RS10645 (CEW83_10635) pilD 2341546..2342397 (+) 852 WP_108949320.1 prepilin peptidase Machinery gene
  CEW83_RS10650 (CEW83_10640) - 2342611..2342892 (+) 282 WP_108949321.1 FmdB family zinc ribbon protein -
  CEW83_RS10655 (CEW83_10645) - 2342917..2343543 (+) 627 WP_108949322.1 DUF502 domain-containing protein -
  CEW83_RS10660 (CEW83_10650) aspS 2343603..2345402 (+) 1800 WP_108949323.1 aspartate--tRNA ligase -
  CEW83_RS10665 (CEW83_10655) - 2345478..2346527 (-) 1050 WP_108949324.1 Glu/Leu/Phe/Val dehydrogenase dimerization domain-containing protein -

Sequence

Protein


Download         Length: 283 a.a.        Molecular weight: 30895.54 Da        Isoelectric Point: 7.0280

>NTDB_id=237464 CEW83_RS10645 WP_108949320.1 2341546..2342397(+) (pilD) [Parazoarcus communis strain TSPY31]
MSEFLSDPLAFTPLVTLLGLFVGSFLNVVIHRLPRMMERDWQVQAAEMRGEPAPESERFNLAAPRSRCPHCGHLITALEN
IPVVSYLFLRGRCGHCSAPISRRYPMVEVLTAVLSGYAAWHFGFGLAAAGALLFLWAMIALAFIDLDTQLLPDDITLPLL
WLGLAFNLAGVYTDLPSAVIGAMAGYLTLWSIFWLFKLATGKEGMGYGDFKLLGAIGAWLGWQTLPLTILLSSVVGAVIG
ISLIVFARHGRNIPIPFGPYLATAGIIALFWGEAITQRYMGVL

Nucleotide


Download         Length: 852 bp        

>NTDB_id=237464 CEW83_RS10645 WP_108949320.1 2341546..2342397(+) (pilD) [Parazoarcus communis strain TSPY31]
ATGTCAGAGTTCCTGAGCGACCCGCTCGCCTTCACCCCACTCGTCACGCTGCTCGGTCTCTTTGTCGGCAGTTTCCTCAA
TGTCGTGATCCATCGCCTGCCGCGGATGATGGAGCGCGACTGGCAGGTGCAGGCGGCGGAAATGCGCGGCGAACCCGCCC
CCGAATCCGAGCGCTTCAACCTCGCCGCCCCCCGGTCACGCTGCCCCCATTGCGGACACCTGATCACCGCGCTTGAAAAC
ATCCCGGTGGTCAGCTATCTGTTCCTGCGTGGCCGTTGCGGCCACTGCTCCGCGCCGATCAGCCGCCGCTACCCCATGGT
GGAAGTGCTCACCGCCGTGCTCTCCGGCTACGCGGCATGGCATTTCGGTTTCGGCCTGGCCGCCGCCGGCGCATTGCTCT
TCCTCTGGGCCATGATCGCGCTTGCCTTCATCGATCTCGACACTCAGTTGCTCCCGGACGACATCACGCTGCCACTGCTC
TGGCTTGGTCTCGCGTTCAACCTCGCCGGCGTCTACACGGACCTGCCGAGCGCAGTGATTGGCGCGATGGCAGGTTACCT
GACGCTGTGGTCGATCTTCTGGCTGTTCAAGCTTGCGACCGGCAAGGAGGGCATGGGCTACGGCGACTTCAAGCTCCTCG
GCGCCATTGGCGCATGGCTTGGCTGGCAGACGCTGCCGCTGACCATTCTTCTCTCCTCGGTCGTCGGGGCGGTGATCGGC
ATCAGCCTGATCGTATTCGCCCGCCACGGCAGAAACATTCCGATACCGTTCGGCCCATATCTCGCGACGGCCGGCATCAT
CGCGCTGTTCTGGGGTGAAGCGATCACACAGCGCTACATGGGCGTGCTCTGA

Domains

Predicted by InterproScan.

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(17-122)

Type IV leader peptidase family (Peptidase_A24)

(133-241)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2U8GQ29

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Vibrio cholerae strain A1552

55.319

99.647

0.551
  pilD Vibrio campbellii strain DS40M4

53.546

99.647

0.534
  pilD Acinetobacter baumannii D1279779

48.763

100

0.488
  pilD Acinetobacter nosocomialis M2

48.41

100

0.484
  pilD Neisseria gonorrhoeae MS11

52.529

90.813

0.477

Multiple sequence alignment