Detailed information    

insolico Bioinformatically predicted

Overview

Name   cinA   Type   Machinery gene
Locus tag   M6_RS08995 Genome accession   NC_006086
Coordinates   1814256..1815527 (-) Length   423 a.a.
NCBI ID   WP_011185061.1    Uniprot ID   Q5X9H9
Organism   Streptococcus pyogenes MGAS10394     
Function   require for competence (predicted from homology)   
Unclear

Related MGE

Note: This gene co-localizes with putative mobile genetic elements (MGEs) in the genome predicted by VRprofile2, as detailed below.

Gene-MGE association summary

MGE type MGE coordinates Gene coordinates Relative position Distance (bp)
Prophage 1803631..1832552 1814256..1815527 within 0


Gene organization within MGE regions


Location: 1803631..1832552
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  M6_RS08940 (M6_Spy1787) yaaA 1803631..1804362 (+) 732 WP_011185056.1 peroxide stress protein YaaA -
  M6_RS08945 (M6_Spy1788) nrdG 1804536..1805150 (-) 615 WP_002982219.1 anaerobic ribonucleoside-triphosphate reductase activating protein -
  M6_RS08950 (M6_Spy1789) - 1805150..1805659 (-) 510 WP_011185058.1 GNAT family N-acetyltransferase -
  M6_RS08955 (M6_Spy1790) - 1805668..1806603 (-) 936 WP_002992828.1 Gfo/Idh/MocA family protein -
  M6_RS10160 (M6_Spy1791) - 1806632..1806778 (-) 147 WP_002982210.1 hypothetical protein -
  M6_RS08960 (M6_Spy1792) nrdD 1806960..1809158 (-) 2199 WP_002992831.1 anaerobic ribonucleoside-triphosphate reductase -
  M6_RS08965 (M6_Spy1793) - 1809255..1810814 (-) 1560 WP_011185060.1 membrane protein -
  M6_RS08970 (M6_Spy1794) - 1811227..1811532 (-) 306 WP_002982199.1 DUF1292 domain-containing protein -
  M6_RS08975 (M6_Spy1795) ruvX 1811544..1811963 (-) 420 WP_002982196.1 Holliday junction resolvase RuvX -
  M6_RS08980 (M6_Spy1796) - 1811960..1812229 (-) 270 WP_002982194.1 IreB family regulatory phosphoprotein -
  M6_RS08985 (M6_Spy1797) spx 1812342..1812740 (-) 399 WP_002982188.1 transcriptional regulator Spx -
  M6_RS08990 (M6_Spy1798) recA 1813031..1814167 (-) 1137 WP_002982186.1 recombinase RecA Machinery gene
  M6_RS08995 (M6_Spy1799) cinA 1814256..1815527 (-) 1272 WP_011185061.1 competence/damage-inducible protein A Machinery gene
  M6_RS09000 (M6_Spy1800) - 1815596..1816156 (-) 561 WP_021340449.1 DNA-3-methyladenine glycosylase I -
  M6_RS09005 (M6_Spy1801) ruvA 1816166..1816762 (-) 597 WP_011185063.1 Holliday junction branch migration protein RuvA -
  M6_RS09010 (M6_Spy1802) - 1816764..1817984 (-) 1221 WP_011018316.1 MDR family MFS transporter -
  M6_RS09015 (M6_Spy1803) mutL 1817995..1819977 (-) 1983 WP_027968888.1 DNA mismatch repair endonuclease MutL -
  M6_RS09020 (M6_Spy1804) - 1820072..1821223 (-) 1152 WP_023078125.1 tyrosine-type recombinase/integrase -
  M6_RS09915 (M6_Spy1805) - 1821545..1821688 (+) 144 WP_011185066.1 putative holin-like toxin -
  M6_RS09025 (M6_Spy1806) - 1822560..1823240 (-) 681 WP_011185067.1 helix-turn-helix domain-containing protein -
  M6_RS09030 (M6_Spy1807) - 1823404..1823601 (+) 198 WP_011185068.1 helix-turn-helix domain-containing protein -
  M6_RS09035 (M6_Spy1808) - 1823615..1824223 (+) 609 WP_014407937.1 phage repressor protein -
  M6_RS09040 (M6_Spy1809) - 1824244..1824651 (+) 408 WP_011185070.1 hypothetical protein -
  M6_RS10340 - 1824664..1824786 (+) 123 WP_021340213.1 hypothetical protein -
  M6_RS09045 (M6_Spy1810) - 1824935..1825456 (+) 522 WP_002992498.1 Rha family transcriptional regulator -
  M6_RS09050 (M6_Spy1811) - 1825701..1826033 (+) 333 WP_002992497.1 hypothetical protein -
  M6_RS09055 (M6_Spy1812) - 1826033..1826224 (+) 192 WP_027968868.1 hypothetical protein -
  M6_RS09060 (M6_Spy1813) - 1826237..1826599 (+) 363 WP_000206026.1 hypothetical protein -
  M6_RS09065 (M6_Spy1814) - 1826596..1826925 (+) 330 WP_000174505.1 hypothetical protein -
  M6_RS09070 (M6_Spy1815) - 1826928..1827200 (+) 273 WP_000051914.1 hypothetical protein -
  M6_RS09075 (M6_Spy1816) - 1827201..1828058 (+) 858 WP_011185073.1 primase alpha helix C-terminal domain-containing protein -
  M6_RS09080 (M6_Spy1817) - 1828027..1829715 (+) 1689 WP_011185074.1 DNA primase family protein -
  M6_RS09920 (M6_Spy1818) - 1830002..1830175 (+) 174 WP_000694577.1 hypothetical protein -
  M6_RS10165 (M6_Spy1819) - 1830181..1830354 (+) 174 WP_011185075.1 hypothetical protein -
  M6_RS09095 (M6_Spy1820) - 1830356..1830865 (+) 510 WP_011185076.1 hypothetical protein -
  M6_RS09100 (M6_Spy1821) - 1830940..1831428 (+) 489 WP_002992480.1 hypothetical protein -
  M6_RS09105 (M6_Spy1822) - 1831832..1832194 (+) 363 WP_002992136.1 DUF1492 domain-containing protein -
  M6_RS09110 (M6_Spy1823) - 1832169..1832552 (+) 384 WP_011185077.1 ArpU family phage packaging/lysis transcriptional regulator -

Sequence

Protein


Download         Length: 423 a.a.        Molecular weight: 45895.59 Da        Isoelectric Point: 4.7648

>NTDB_id=23475 M6_RS08995 WP_011185061.1 1814256..1815527(-) (cinA) [Streptococcus pyogenes MGAS10394]
MKAELIAVGTEILTGQIVNTNAQFLSEKMAELGIDVYFQTAVGDNEERLLSVITTASQRSDLVILCGGLGPTKDDLTKQT
LAKYLRKDLVYDEQACQKLDDFFAKRKPSSRTPNNERQAQVIEGSIPLPNKTGLAVGGFITVDGISYVVLPGPPSELKSM
VNEELVPLLSKQYSTLYSKVLRFFGVGESQLVTVLSDFIENQTDPTIAPYAKTGEVTLRLSTKTENQALADKKLGQLEAQ
LLSRKTLEGQPLADVFYGYGEDNSLARETFELLVKYDKTITAAESLTAGLFQSTLASFPGASQVFNGGFVAYSMEEKAKM
LGLPLEELKSHGVVSAYTAEGMAEQARLLTGADIGVSLTGVAGPDMLEEQPAGTVFIGLATQTKVESIKVLISGQSRLDV
RYIATLHAFNMVRKTLLKLENLL

Nucleotide


Download         Length: 1272 bp        

>NTDB_id=23475 M6_RS08995 WP_011185061.1 1814256..1815527(-) (cinA) [Streptococcus pyogenes MGAS10394]
ATGAAAGCTGAACTGATTGCAGTAGGTACCGAAATTTTGACTGGTCAAATTGTGAATACCAATGCTCAATTTCTGTCGGA
AAAAATGGCAGAGCTAGGTATTGATGTCTATTTTCAAACGGCTGTTGGGGACAACGAGGAGCGTTTACTTTCAGTGATTA
CAACTGCTAGTCAGCGTAGTGACTTGGTGATTTTATGTGGTGGCCTTGGTCCAACGAAAGATGATTTAACCAAACAAACT
TTAGCAAAGTACCTTAGGAAAGACTTGGTTTATGATGAGCAAGCTTGTCAGAAACTAGATGACTTTTTTGCTAAGCGCAA
GCCTTCATCACGGACACCAAATAATGAGCGACAGGCACAAGTGATTGAAGGGTCAATCCCTTTGCCAAATAAAACTGGTC
TTGCGGTTGGTGGGTTCATCACAGTCGATGGTATTAGTTATGTTGTCTTACCGGGTCCTCCAAGTGAATTGAAGTCGATG
GTAAATGAAGAATTGGTACCACTTCTGTCAAAACAATACAGTACATTGTATTCAAAGGTACTACGCTTTTTTGGTGTTGG
GGAAAGTCAGTTGGTAACAGTTTTGTCAGATTTTATTGAGAATCAAACTGATCCAACCATTGCTCCGTATGCTAAGACTG
GCGAAGTGACTCTTCGCTTATCAACAAAAACTGAAAACCAAGCTCTGGCAGATAAAAAGTTAGGTCAGCTAGAAGCGCAG
CTACTATCCCGAAAAACTCTTGAAGGTCAACCCTTAGCTGATGTCTTTTATGGCTATGGGGAGGATAATTCCTTAGCGCG
TGAGACATTTGAGCTCTTAGTAAAATATGATAAGACAATTACAGCAGCAGAAAGTCTAACCGCGGGATTATTTCAGTCAA
CTTTGGCGAGTTTTCCAGGAGCTTCTCAAGTATTCAATGGAGGCTTTGTGGCTTATAGCATGGAAGAAAAAGCGAAAATG
CTAGGCCTTCCTTTAGAGGAGTTGAAATCGCATGGCGTTGTTAGTGCTTATACAGCCGAGGGGATGGCGGAGCAAGCAAG
GTTATTGACTGGTGCTGATATTGGGGTAAGTTTAACAGGTGTTGCCGGACCAGATATGTTGGAGGAACAGCCTGCAGGTA
CAGTTTTCATTGGCCTTGCCACTCAAACTAAGGTAGAATCAATAAAGGTTTTGATTAGCGGGCAAAGTCGTTTGGATGTG
CGCTATATCGCCACTTTACATGCCTTTAATATGGTCCGTAAAACTTTATTAAAACTTGAGAATTTGCTATAA

Domains

Predicted by InterproScan.

Competence-damaged protein (CinA)

(263-416)

Damage-inducible protein CinA KH domain (CinA_KH)

(178-243)

Probable molybdopterin binding domain (MoCF_biosynth)

(5-170)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q5X9H9

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  cinA Streptococcus mutans UA159

68.794

100

0.688
  cinA Streptococcus mitis SK321

69.065

98.582

0.681
  cinA Streptococcus mitis NCTC 12261

68.585

98.582

0.676
  cinA Streptococcus pneumoniae TIGR4

67.626

98.582

0.667
  cinA Streptococcus pneumoniae Rx1

67.626

98.582

0.667
  cinA Streptococcus pneumoniae R6

67.626

98.582

0.667
  cinA Streptococcus pneumoniae D39

67.386

98.582

0.664
  cinA Streptococcus suis isolate S10

52.885

98.345

0.52
  cinA Bacillus subtilis subsp. subtilis str. 168

46.172

98.818

0.456

Multiple sequence alignment