Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   CDA09_RS17700 Genome accession   NZ_CP021731
Coordinates   3825810..3827531 (+) Length   573 a.a.
NCBI ID   WP_121429855.1    Uniprot ID   -
Organism   Azoarcus sp. DN11     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3820810..3832531
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CDA09_RS17675 (CDA09_17650) - 3821154..3821930 (-) 777 WP_174718452.1 SprT family zinc-dependent metalloprotease -
  CDA09_RS17680 (CDA09_17655) - 3821927..3822679 (-) 753 WP_121429851.1 molybdopterin-binding protein -
  CDA09_RS17685 (CDA09_17660) - 3822683..3823453 (-) 771 WP_121429852.1 EI24 domain-containing protein -
  CDA09_RS17690 (CDA09_17665) ffh 3823468..3824826 (-) 1359 WP_121429853.1 signal recognition particle protein -
  CDA09_RS17695 (CDA09_17670) ccsA 3824869..3825696 (+) 828 WP_121429854.1 cytochrome c biogenesis protein CcsA -
  CDA09_RS17700 (CDA09_17675) pilB 3825810..3827531 (+) 1722 WP_121429855.1 type IV-A pilus assembly ATPase PilB Machinery gene
  CDA09_RS17705 (CDA09_17680) pilC 3827548..3828783 (+) 1236 WP_121429856.1 type II secretion system F family protein Machinery gene
  CDA09_RS17710 (CDA09_17685) pilD 3828839..3829690 (+) 852 WP_121429857.1 A24 family peptidase Machinery gene
  CDA09_RS17715 (CDA09_17690) - 3829814..3830077 (+) 264 WP_121429858.1 zinc ribbon domain-containing protein -
  CDA09_RS17720 (CDA09_17695) - 3830093..3830719 (+) 627 WP_121429859.1 DUF502 domain-containing protein -

Sequence

Protein


Download         Length: 573 a.a.        Molecular weight: 62435.69 Da        Isoelectric Point: 5.3389

>NTDB_id=232922 CDA09_RS17700 WP_121429855.1 3825810..3827531(+) (pilB) [Azoarcus sp. DN11]
MAANPQTALSGLARALIQHGRLSEADAVACTKSAAVVPNGFILELAQRKLLTAKAVAWFAAETFGYPLLDIASVDRASIP
RDAIDRKLMAKHQVVALGKRQNRLTVATADPSNMRALDEIRFQTGMQVDLIVVEADRLARLAESLSESTEDTLKELTGEE
FDMDLLQDQEAAEQTAPPDDASAEVDDAPVVKFIQKVLIDAINEGASDIHFEPYEKYYRIRVRTDGVLREIAQPPLVLKE
KIAARIKVISRLDISEKRIPQDGRMKLVLSKNKSIDFRVSSLPTLHGEKIVMRILDASSAMLGVDALGYEPDQKKQLLDA
IERPYGMILVTGPTGSGKTVSLYTCLNILNKAGVNISTAEDPAEINLPGINQVNVNEKAGLTFAASLRAFLRQDPDVIMV
GEIRDLETAEISIKAAQTGHLVLSTLHTNDAPTTLERLRNMGVAPFNIASSVILITAQRLARRLCTCKQPVDIPIEALLQ
AGFSGEELDGSWRPYGPVGCERCKGSGYKGRLGIYQVMPISEEIAHIIMTNGNSMDIAAQAQREGVRDLRQSGLLKVKQG
ATSLEEVLATTNE

Nucleotide


Download         Length: 1722 bp        

>NTDB_id=232922 CDA09_RS17700 WP_121429855.1 3825810..3827531(+) (pilB) [Azoarcus sp. DN11]
ATGGCAGCAAACCCCCAGACCGCCCTGAGCGGACTCGCGCGAGCACTGATCCAGCACGGCCGGCTCTCGGAGGCCGACGC
CGTGGCGTGCACCAAAAGCGCAGCCGTAGTTCCCAACGGATTCATCCTCGAACTGGCGCAGCGCAAACTGCTGACCGCCA
AGGCCGTCGCCTGGTTCGCCGCCGAGACATTCGGTTACCCGCTGCTGGACATCGCGTCGGTCGACCGCGCCAGCATCCCT
CGCGACGCCATCGACCGCAAGCTGATGGCCAAGCACCAGGTGGTCGCGCTGGGCAAACGCCAGAATCGCCTCACCGTCGC
GACGGCCGACCCGTCCAACATGCGGGCGCTCGACGAGATCCGTTTCCAGACGGGGATGCAGGTCGATCTCATCGTCGTCG
AGGCCGACCGCCTCGCGCGCCTCGCCGAGTCGCTGTCCGAATCGACCGAGGATACGCTCAAGGAACTCACGGGCGAAGAG
TTCGACATGGACCTCCTGCAGGATCAGGAGGCAGCGGAACAGACGGCGCCCCCCGATGACGCCTCCGCCGAGGTCGACGA
CGCCCCGGTCGTCAAGTTCATCCAGAAAGTCCTGATCGACGCGATCAACGAAGGTGCCTCCGACATCCATTTCGAACCTT
ACGAAAAGTACTATCGTATCCGCGTGCGCACCGACGGCGTCCTGCGCGAGATCGCCCAGCCGCCGCTGGTGCTGAAGGAA
AAGATCGCCGCGCGCATCAAGGTCATCTCGCGCCTGGACATCTCCGAGAAGCGCATCCCCCAGGACGGGCGCATGAAACT
CGTCCTCTCGAAGAACAAGTCCATCGATTTCCGCGTGTCCTCGCTGCCGACCCTGCACGGCGAGAAGATCGTCATGCGTA
TTCTCGACGCCTCCTCCGCGATGCTGGGCGTCGATGCGCTCGGCTACGAGCCCGACCAGAAGAAGCAACTGCTCGATGCG
ATCGAGCGCCCCTACGGCATGATCCTGGTGACCGGCCCGACCGGTTCGGGCAAGACGGTGTCGCTCTACACCTGCCTGAA
TATCCTCAACAAGGCCGGCGTGAACATCTCGACCGCAGAGGATCCGGCGGAAATCAACCTGCCCGGCATCAACCAGGTCA
ACGTCAACGAGAAGGCGGGCCTCACCTTCGCGGCATCGCTGCGCGCCTTCCTGCGCCAGGATCCGGACGTGATCATGGTC
GGCGAGATCCGCGACCTCGAGACGGCGGAGATCTCGATCAAGGCCGCCCAGACGGGTCACCTCGTGCTGTCGACGCTGCA
CACCAACGACGCCCCGACCACGCTCGAGCGCCTGCGCAACATGGGCGTCGCACCGTTCAACATCGCGTCCTCGGTGATCC
TGATCACCGCCCAGCGCTTAGCACGGCGGCTGTGCACGTGCAAGCAGCCGGTCGACATCCCCATCGAAGCCTTGCTCCAG
GCGGGCTTCTCCGGCGAGGAGCTCGACGGCAGCTGGCGGCCCTACGGTCCCGTCGGGTGCGAACGCTGCAAGGGCAGCGG
CTACAAGGGGCGCCTCGGCATTTATCAGGTCATGCCCATTTCCGAGGAAATTGCCCATATCATCATGACCAATGGCAATT
CCATGGACATCGCCGCACAGGCCCAGCGCGAGGGCGTGCGCGACCTGCGCCAGTCCGGCCTGCTGAAAGTGAAACAGGGT
GCGACCTCGCTCGAGGAAGTTCTCGCCACCACCAACGAATAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(196-463)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(45-149)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

55.458

99.127

0.55
  pilB Acinetobacter baumannii D1279779

54.577

99.127

0.541
  pilF Neisseria gonorrhoeae MS11

56.604

92.496

0.524
  pilB Legionella pneumophila strain ERS1305867

52.817

99.127

0.524
  pilB Vibrio cholerae strain A1552

48.313

98.255

0.475
  pilB Vibrio parahaemolyticus RIMD 2210633

46.967

100

0.473
  pilB Vibrio campbellii strain DS40M4

46.655

96.51

0.45
  pilF Thermus thermophilus HB27

40.111

94.415

0.379
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.525

93.019

0.377
  pilB/pilB1 Synechocystis sp. PCC 6803

41.683

87.086

0.363

Multiple sequence alignment