Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   B4U42_RS11035 Genome accession   NZ_CP021644
Coordinates   2141048..2142433 (-) Length   461 a.a.
NCBI ID   WP_005711779.1    Uniprot ID   -
Organism   Glaesserella parasuis 29755     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2136048..2147433
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  B4U42_RS11010 (B4U42_10865) clpP 2136065..2136646 (+) 582 WP_005711766.1 ATP-dependent Clp endopeptidase proteolytic subunit ClpP -
  B4U42_RS11015 (B4U42_10870) clpX 2136653..2137903 (+) 1251 WP_005711769.1 ATP-dependent protease ATP-binding subunit ClpX -
  B4U42_RS11020 (B4U42_10875) dapE 2137977..2139110 (-) 1134 WP_005711772.1 succinyl-diaminopimelate desuccinylase -
  B4U42_RS11025 (B4U42_10880) - 2139194..2139862 (-) 669 WP_005711775.1 prepilin peptidase -
  B4U42_RS11030 (B4U42_10885) pilC 2139859..2141055 (-) 1197 WP_005711777.1 type II secretion system F family protein Machinery gene
  B4U42_RS11035 (B4U42_10890) pilB 2141048..2142433 (-) 1386 WP_005711779.1 GspE/PulE family protein Machinery gene
  B4U42_RS11040 (B4U42_10895) pilA 2142481..2142945 (-) 465 WP_005711781.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  B4U42_RS11045 (B4U42_10900) radA 2143133..2144509 (+) 1377 WP_005711784.1 DNA repair protein RadA -
  B4U42_RS11050 (B4U42_10905) gmk 2144562..2145188 (-) 627 WP_005711785.1 guanylate kinase -
  B4U42_RS11055 (B4U42_10910) - 2145257..2146228 (-) 972 WP_005714915.1 IS110 family transposase -
  B4U42_RS11060 (B4U42_10915) gpt 2146629..2147108 (-) 480 WP_005714020.1 xanthine phosphoribosyltransferase -

Sequence

Protein


Download         Length: 461 a.a.        Molecular weight: 52772.61 Da        Isoelectric Point: 5.2577

>NTDB_id=232177 B4U42_RS11035 WP_005711779.1 2141048..2142433(-) (pilB) [Glaesserella parasuis 29755]
MSQYTICEQQTERIFDISPSRWQKNCEEKQLLLRYLAVPVQETEHKLWLAVDDENNLTACEIFAFMTHKQIEPVVIASDE
LKYLLNALSPEQQPIYEESELAFVEQEQEQLNLNDPIIQLLDNLFKFCLAQNASDIHIEPRKQKLIIRLRIDGVLHLYKS
LSIQLASRLISRIKLLAKLDISELRQPQDGQFSFTTALADTLDFRVSSLPTIYGEKLVLRLQKNKPTSFDFLQLGFNPQQ
QTTLLNALKQPQGLILVTGPTGSGKSITLYSALNYLNQMDKHILTAEDPVEIEIDGLIQTQVNRSIQLDFSQLLRTFLRQ
DPDIIMLGEIRDEESAKMALRASQTGHLVLSTLHTNDALSAVERLLQLGIQEYEIQNSLLLVIAQRLVRKLCKKCRGVGC
DECYQGYRGRIGVYQCLSRTAKKFDKQTAYLDFSSLRESAKQKIEQQLTNEIEVDRVLGDE

Nucleotide


Download         Length: 1386 bp        

>NTDB_id=232177 B4U42_RS11035 WP_005711779.1 2141048..2142433(-) (pilB) [Glaesserella parasuis 29755]
ATGTCCCAATACACTATCTGCGAACAACAAACCGAACGCATTTTTGATATATCGCCTAGCCGTTGGCAAAAAAATTGCGA
GGAGAAACAGCTTTTATTGCGTTACCTTGCCGTTCCTGTGCAAGAAACTGAGCATAAATTATGGCTTGCCGTTGATGATG
AAAACAATCTCACCGCTTGCGAAATCTTTGCTTTTATGACCCATAAGCAGATTGAGCCAGTGGTGATTGCTTCTGATGAA
CTGAAATACTTGCTTAACGCTCTCTCGCCTGAACAACAGCCCATTTATGAAGAGAGCGAGCTGGCTTTTGTTGAACAGGA
GCAAGAACAGCTCAACCTAAACGATCCGATTATTCAACTGCTAGATAATCTCTTTAAATTTTGTCTAGCTCAGAATGCGT
CCGACATTCATATTGAGCCACGCAAGCAAAAATTGATTATCCGCTTACGCATTGACGGCGTTTTGCACCTGTATAAAAGC
CTATCTATTCAGCTGGCAAGCCGACTAATTTCACGGATAAAACTACTGGCAAAGCTAGATATTAGCGAATTACGCCAGCC
ACAAGACGGGCAATTTAGTTTTACCACCGCCCTTGCGGATACCCTTGATTTTCGGGTGTCTAGCCTGCCGACGATCTACG
GTGAAAAACTGGTATTACGCTTACAAAAAAACAAGCCGACCAGTTTTGATTTTCTACAACTTGGCTTTAATCCACAACAG
CAAACCACACTGCTCAACGCCCTGAAACAACCACAGGGGCTGATTTTAGTGACCGGACCAACGGGCAGTGGCAAAAGCAT
CACCCTGTACAGTGCCTTAAACTATCTTAACCAAATGGATAAACATATTCTCACCGCCGAAGATCCGGTGGAAATCGAGA
TTGACGGCTTAATTCAAACCCAAGTTAATCGCAGTATTCAACTGGATTTTAGCCAGCTGTTACGCACCTTTCTACGTCAA
GATCCCGACATCATTATGTTAGGCGAAATTCGAGATGAAGAGAGTGCCAAAATGGCGTTACGAGCAAGCCAGACGGGGCA
TTTAGTCCTTTCGACCCTGCATACCAACGACGCACTCTCTGCGGTCGAACGCTTGTTGCAACTGGGTATTCAAGAGTATG
AAATCCAAAATTCACTACTGTTAGTGATTGCACAACGCCTTGTGCGTAAGCTCTGTAAAAAGTGTCGTGGAGTAGGCTGT
GACGAGTGCTATCAAGGCTATAGAGGGCGGATCGGGGTTTACCAATGTTTAAGCAGAACGGCAAAAAAATTCGATAAACA
GACCGCTTACTTGGATTTTTCCAGCCTACGAGAGAGTGCTAAACAGAAAATTGAGCAACAACTAACCAACGAAATCGAAG
TGGATAGGGTATTAGGCGATGAATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(30-92)

Type II/IV secretion system protein (T2SSE)

(129-390)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Glaesserella parasuis strain SC1401

99.566

100

0.996
  pilB Haemophilus influenzae Rd KW20

58.093

97.831

0.568
  pilB Haemophilus influenzae 86-028NP

57.456

98.915

0.568
  pilB Vibrio cholerae strain A1552

38.353

100

0.414
  pilB Vibrio parahaemolyticus RIMD 2210633

39.293

100

0.41
  pilB Vibrio campbellii strain DS40M4

39.085

100

0.408
  pilB Acinetobacter baumannii D1279779

36.187

100

0.403
  pilB Acinetobacter baylyi ADP1

36.634

100

0.401
  pilB Legionella pneumophila strain ERS1305867

40.682

95.445

0.388
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.248

100

0.388
  pilF Neisseria gonorrhoeae MS11

36.421

100

0.375

Multiple sequence alignment