Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   BJE04_RS13220 Genome accession   NC_005139
Coordinates   2834105..2835793 (+) Length   562 a.a.
NCBI ID   WP_011151129.1    Uniprot ID   Q7MHT8
Organism   Vibrio vulnificus YJ016     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2829105..2840793
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BJE04_RS13200 (VV2774) pdhR 2830678..2831445 (-) 768 WP_011079554.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -
  BJE04_RS13205 (VV2776) ampD 2831834..2832406 (-) 573 WP_011151126.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  BJE04_RS13210 (VV2777) nadC 2832499..2833386 (+) 888 WP_043877312.1 carboxylating nicotinate-nucleotide diphosphorylase -
  BJE04_RS13215 (VV2778) - 2833606..2834088 (+) 483 WP_011151128.1 pilin -
  BJE04_RS13220 (VV2779) pilB 2834105..2835793 (+) 1689 WP_011151129.1 type IV-A pilus assembly ATPase PilB Machinery gene
  BJE04_RS13225 (VV2780) pilC 2835841..2837067 (+) 1227 WP_011151130.1 type II secretion system F family protein Machinery gene
  BJE04_RS13230 (VV2781) pilD 2837161..2838030 (+) 870 WP_043877313.1 prepilin peptidase Machinery gene
  BJE04_RS13235 (VV2782) coaE 2838032..2838640 (+) 609 WP_011151132.1 dephospho-CoA kinase -
  BJE04_RS13240 (VV2783) zapD 2838673..2839413 (+) 741 WP_011079546.1 cell division protein ZapD -
  BJE04_RS13245 (VV2784) yacG 2839476..2839670 (+) 195 WP_026060801.1 DNA gyrase inhibitor YacG -
  BJE04_RS13250 (VV2785) rplS 2839943..2840296 (-) 354 WP_011079544.1 50S ribosomal protein L19 -

Sequence

Protein


Download         Length: 562 a.a.        Molecular weight: 62452.48 Da        Isoelectric Point: 5.8187

>NTDB_id=23140 BJE04_RS13220 WP_011151129.1 2834105..2835793(+) (pilB) [Vibrio vulnificus YJ016]
MHSNLATILRQAKQLSLTQEQACRETIQASGVTAPEALLQLGFFQPDELTEQLSAIFGLPRTDLNQYDYQELCNQLGLRE
LITRHQALPLQKSNAGLILAVADPTNIQAEDDFRFATGVQVEIVLADCQELQAAIRRLYGKSIKQSQGSGLKEIHQDELA
NLVEVSDDEVESIEDLSQDESPVSRFINQILLDAVRKGASDIHFEPYENQYRVRLRCDGILVEVQQPPSHLSRRLSARIK
ILSKLDIAERRLPQDGRIKLRLNQETAIDMRVSTLPTLFGEKIVLRLLDSSSSSLDIDKLGYNPQQKQLYLDALRKPQGM
ILMTGPTGSGKTVSLYTGLSLLNRPEVNISTAEDPVEINLPGINQVQVKPKIGFGFAEALRSFLRQDPDIVMVGEIRDLE
TAEIAVKAAQTGHLVLSTLHTNSAAETIVRLSNMGIASFNLASSLSLIIAQRLARRLCSHCKQPHPYNATFELLGLKADD
LIYQADRQGCHHCTHGYSGRVGIYEVMPFDEQLAEAIVKGASVQSLEMLARQKGMMTLKDSGLEKLKQGITSLEELQRVL
YL

Nucleotide


Download         Length: 1689 bp        

>NTDB_id=23140 BJE04_RS13220 WP_011151129.1 2834105..2835793(+) (pilB) [Vibrio vulnificus YJ016]
ATGCACAGCAATCTTGCCACTATTCTGCGTCAGGCCAAACAACTTAGCCTAACGCAAGAGCAAGCGTGTCGAGAGACAAT
ACAAGCTTCCGGTGTCACAGCACCGGAAGCTTTGTTGCAACTGGGCTTTTTCCAGCCTGATGAACTAACCGAACAGCTAA
GCGCGATTTTTGGCTTACCACGCACTGACCTCAACCAATATGACTACCAAGAGCTGTGTAATCAACTTGGGTTGCGTGAG
CTGATCACTCGTCACCAAGCACTGCCACTGCAAAAAAGCAACGCTGGTTTAATACTCGCGGTTGCAGACCCGACCAATAT
TCAAGCTGAAGATGATTTCCGTTTTGCCACAGGCGTGCAGGTGGAGATTGTTTTGGCCGATTGCCAAGAGCTGCAAGCGG
CGATTCGTAGACTCTATGGAAAGAGCATCAAACAGAGCCAAGGCTCTGGTTTAAAAGAGATTCACCAAGATGAGCTGGCG
AATTTGGTTGAGGTTTCAGATGACGAAGTCGAGTCTATTGAAGACTTAAGCCAAGATGAATCCCCCGTTAGCCGCTTTAT
CAACCAAATTCTGCTGGATGCGGTACGTAAAGGCGCGTCCGATATTCATTTTGAACCGTACGAAAACCAATACCGCGTGC
GATTGCGTTGCGATGGTATTTTGGTGGAAGTGCAGCAGCCCCCAAGCCATTTAAGCCGCCGTTTATCCGCGAGGATCAAG
ATCCTCTCCAAGTTGGATATTGCGGAAAGGCGTCTGCCACAAGATGGACGGATTAAGCTGCGCCTCAACCAAGAAACCGC
GATTGATATGCGCGTTTCCACCTTACCCACACTGTTTGGTGAAAAAATTGTGTTGCGACTTCTCGACAGCTCGAGCAGTA
GCTTGGATATTGATAAGTTAGGCTACAACCCGCAACAAAAGCAGCTCTACCTCGATGCCCTGCGCAAACCGCAAGGGATG
ATTTTGATGACGGGCCCAACAGGAAGCGGCAAAACCGTTTCGCTGTACACAGGCTTGAGTTTGCTTAACCGGCCAGAAGT
GAATATTTCCACCGCGGAAGACCCAGTCGAGATTAACCTCCCGGGAATCAACCAAGTGCAAGTGAAACCCAAAATTGGTT
TTGGTTTTGCAGAAGCATTGCGTTCTTTTCTCCGTCAAGACCCGGATATCGTCATGGTGGGGGAAATTCGAGATTTGGAA
ACCGCCGAGATCGCCGTCAAAGCAGCCCAAACCGGTCACCTTGTGCTATCCACCTTACATACCAACTCGGCTGCTGAAAC
CATTGTTCGTCTATCTAACATGGGGATCGCCAGCTTTAACTTGGCCTCATCCTTGAGTTTGATCATTGCTCAGCGTCTGG
CTCGTCGGTTATGCAGCCATTGCAAGCAACCTCATCCATACAACGCAACATTTGAGTTACTCGGCCTAAAAGCTGACGAC
TTGATTTATCAAGCTGATAGACAGGGTTGCCATCATTGTACTCATGGTTATTCCGGTCGTGTCGGTATATACGAAGTCAT
GCCTTTTGATGAACAGCTTGCAGAGGCGATCGTTAAAGGCGCCTCGGTACAAAGCTTAGAGATGCTTGCTCGACAAAAAG
GAATGATGACACTCAAAGATTCCGGCCTTGAAAAACTCAAACAGGGCATCACCAGCTTAGAAGAGCTGCAACGCGTCCTC
TATTTGTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(38-141)

Type II/IV secretion system protein (T2SSE)

(189-456)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB Q7MHT8

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio campbellii strain DS40M4

76.512

100

0.765
  pilB Vibrio parahaemolyticus RIMD 2210633

76.157

100

0.762
  pilB Vibrio cholerae strain A1552

74.423

100

0.746
  pilB Legionella pneumophila strain ERS1305867

53.216

91.281

0.486
  pilB Acinetobacter baumannii D1279779

51.538

92.527

0.477
  pilB Acinetobacter baylyi ADP1

50.478

93.06

0.47
  pilF Neisseria gonorrhoeae MS11

45.31

100

0.456
  pilF Thermus thermophilus HB27

40.395

99.11

0.4
  pilB/pilB1 Synechocystis sp. PCC 6803

36.888

100

0.375
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.491

94.306

0.363

Multiple sequence alignment