Detailed information NTG0023142

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: BJE04_RS13230
• Genome accession: NC_005139
• Coordinates: 2837161..2838030 (+)
• Length: 289 a.a.
• NCBI ID: WP_043877313.1
• Uniprot ID: B7U4T2
• Organism: Vibrio vulnificus YJ016
• Function: type IV pilus biogenesis and function (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2832161..2843030

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
BJE04_RS13210 (VV2777)	nadC	2832499..2833386 (+)	888	WP_043877312.1	carboxylating nicotinate-nucleotide diphosphorylase	-
BJE04_RS13215 (VV2778)	-	2833606..2834088 (+)	483	WP_011151128.1	pilin	-
BJE04_RS13220 (VV2779)	pilB	2834105..2835793 (+)	1689	WP_011151129.1	type IV-A pilus assembly ATPase PilB	Machinery gene
BJE04_RS13225 (VV2780)	pilC	2835841..2837067 (+)	1227	WP_011151130.1	type II secretion system F family protein	Machinery gene
BJE04_RS13230 (VV2781)	pilD	2837161..2838030 (+)	870	WP_043877313.1	prepilin peptidase	Machinery gene
BJE04_RS13235 (VV2782)	coaE	2838032..2838640 (+)	609	WP_011151132.1	dephospho-CoA kinase	-
BJE04_RS13240 (VV2783)	zapD	2838673..2839413 (+)	741	WP_011079546.1	cell division protein ZapD	-
BJE04_RS13245 (VV2784)	yacG	2839476..2839670 (+)	195	WP_026060801.1	DNA gyrase inhibitor YacG	-
BJE04_RS13250 (VV2785)	rplS	2839943..2840296 (-)	354	WP_011079544.1	50S ribosomal protein L19	-
BJE04_RS13255 (VV2786)	trmD	2840338..2841087 (-)	750	WP_011079543.1	tRNA (guanosine(37)-N1)-methyltransferase TrmD	-
BJE04_RS13260 (VV2787)	rimM	2841115..2841663 (-)	549	WP_011079542.1	ribosome maturation factor RimM	-
BJE04_RS13265 (VV2788)	rpsP	2841679..2841927 (-)	249	WP_011079541.1	30S ribosomal protein S16	-

Sequence

Protein

• Length: 289 a.a.
• Molecular weight: 32231.26 Da
• Isoelectric Point: 6.4630

>NTDB_id=23142 BJE04_RS13230 WP_043877313.1 2837161..2838030(+) (pilD) [Vibrio vulnificus YJ016]
MDALQYYPWLYIGLASLFGLLVGSFLNVVIYRLPKIMELEWRQECAESFPEYNITPPTETLTLSTPRSSCPSCHTPIRVR
DNIPVFSWLALRGKCHHCQTKISARYPFVEALSAFLCGLVAWKFGYAPITVALIGFTLVLIAATFIDLDTMLLPDQLTLP
LTWAGIALALLEISPVSLQDSVIGAMAGYLCLWSVYHLFRLLTGKEGMGYGDFKLLAALGAWLGWQYLPMIILLSSVVGL
IFGLIQLRLQKQGIEMAFPFGPYLAIAGWVALMWGDSLMSWYLNYLIGA

Nucleotide

• Length: 870 bp

>NTDB_id=23142 BJE04_RS13230 WP_043877313.1 2837161..2838030(+) (pilD) [Vibrio vulnificus YJ016]
ATGGACGCGTTACAATACTACCCTTGGCTTTATATTGGTTTAGCTTCCCTGTTTGGCTTACTGGTAGGCAGCTTTCTTAA
CGTCGTCATCTACCGTCTGCCGAAAATAATGGAACTGGAATGGCGCCAAGAGTGTGCAGAGTCCTTTCCTGAATACAACA
TCACACCACCGACAGAAACCCTAACATTAAGCACGCCGCGCTCAAGTTGCCCTTCGTGCCATACCCCAATACGAGTTCGA
GATAATATTCCTGTCTTTAGTTGGTTAGCACTAAGAGGAAAATGTCACCATTGCCAGACGAAAATCAGTGCTCGCTATCC
TTTTGTTGAAGCACTGAGCGCCTTTTTGTGCGGCCTAGTCGCTTGGAAGTTCGGTTATGCACCGATTACCGTAGCACTGA
TTGGTTTTACTTTGGTGTTGATCGCCGCCACCTTCATCGACTTAGATACCATGCTACTGCCTGATCAGCTGACACTGCCG
CTGACGTGGGCAGGCATCGCGCTAGCGTTACTAGAGATAAGCCCAGTATCTCTGCAAGATTCAGTGATCGGTGCGATGGC
AGGCTATCTCTGCCTTTGGAGCGTTTATCACCTTTTCCGATTGCTGACAGGGAAAGAAGGCATGGGTTACGGAGACTTTA
AATTGCTTGCGGCACTTGGAGCTTGGCTCGGCTGGCAATATCTACCGATGATCATCTTACTTTCTTCGGTTGTCGGGCTG
ATCTTTGGTCTCATTCAACTCCGATTGCAAAAGCAAGGCATTGAGATGGCTTTTCCATTTGGGCCTTATCTGGCCATTGC
TGGTTGGGTCGCCTTAATGTGGGGAGATAGCTTGATGTCTTGGTATCTCAACTATCTTATAGGGGCATAA

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (17-124)

• Type IV leader peptidase family (Peptidase_A24) (137-244)

3D structure

• AlphaFold DB: B7U4T2

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio campbellii strain DS40M4	77.431	99.654	0.772
pilD	Vibrio cholerae strain A1552	71.875	99.654	0.716
pilD	Acinetobacter nosocomialis M2	54.348	95.502	0.519
pilD	Acinetobacter baumannii D1279779	54.348	95.502	0.519
pilD	Neisseria gonorrhoeae MS11	48	95.156	0.457