Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   CCO03_RS15775 Genome accession   NZ_CP021455
Coordinates   3697782..3699515 (+) Length   577 a.a.
NCBI ID   WP_236904156.1    Uniprot ID   -
Organism   Comamonas serinivorans strain DSM 26136     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3692782..3704515
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CCO03_RS15750 (CCO03_15750) accC 3693124..3694476 (-) 1353 WP_087282574.1 acetyl-CoA carboxylase biotin carboxylase subunit -
  CCO03_RS15755 (CCO03_15755) accB 3694486..3694938 (-) 453 WP_087282576.1 acetyl-CoA carboxylase biotin carboxyl carrier protein -
  CCO03_RS15760 (CCO03_15760) - 3695047..3695430 (-) 384 WP_236903884.1 TlpA family protein disulfide reductase -
  CCO03_RS15765 (CCO03_15765) - 3695577..3696182 (-) 606 WP_087282580.1 hypothetical protein -
  CCO03_RS15770 (CCO03_15770) mpl 3696262..3697647 (+) 1386 WP_087282582.1 UDP-N-acetylmuramate:L-alanyl-gamma-D-glutamyl- meso-diaminopimelate ligase -
  CCO03_RS15775 (CCO03_15775) pilB 3697782..3699515 (+) 1734 WP_236904156.1 type IV-A pilus assembly ATPase PilB Machinery gene
  CCO03_RS15780 (CCO03_15780) pilC 3699535..3700752 (+) 1218 WP_087282585.1 type II secretion system F family protein Machinery gene
  CCO03_RS15785 (CCO03_15785) pilD 3700753..3701622 (+) 870 WP_236903886.1 prepilin peptidase Machinery gene
  CCO03_RS15790 (CCO03_15790) coaE 3701619..3702224 (+) 606 WP_087282587.1 dephospho-CoA kinase -
  CCO03_RS15795 (CCO03_15795) zapD 3702350..3703111 (+) 762 WP_087284808.1 cell division protein ZapD -
  CCO03_RS15800 (CCO03_15800) - 3703115..3703342 (+) 228 WP_087282589.1 DNA gyrase inhibitor YacG -
  CCO03_RS15805 (CCO03_15805) - 3703695..3704168 (-) 474 WP_087282591.1 NUDIX domain-containing protein -

Sequence

Protein


Download         Length: 577 a.a.        Molecular weight: 62603.75 Da        Isoelectric Point: 5.0262

>NTDB_id=230871 CCO03_RS15775 WP_236904156.1 3697782..3699515(+) (pilB) [Comamonas serinivorans strain DSM 26136]
MESTLASKSFATALPGLGRALIAAGRVDEATAGKAFEQVKGDTTAFLPELISRGVVTASEVAHTLADTLAAPLLDLASLD
YDSLPKDLIDAKLARQYAVLPLAKKRGRLVVATADPTLQEAAEKIKFTTQLGVEWVVAEYDKLQEAIAAVTGSANEAVGN
FIATDFGFDDASFATDGEDDSGKDDTSDVEDAPIVRFLHKMLVDAYNMRASDLHFEPFEHSYRVRFRIDGELREITSLPP
GIKEKLASRIKVISRLDISEKRVPQDGRMKLKLGPERVIDFRVSTLPTLFGEKIVIRILDPSSAKMGIDALGYEPIEKER
LLEAIARPYGMILVTGPTGSGKTVSLYTCLNLLNKPGVNISTAEDPSEINLPGVNQVNVNEKAGLTFATALKAFLRQDPD
IIMVGEIRDLETADIAIKAAQTGHLVLSTLHTNDAPTTLTRMRNMGIAPFNIASSVILITAQRLGRRLCPACKEPIDVPR
DALLDAGFDEEELDGSWVPYRPVGCGKCNNGYKGRVGIYEVMPITEDLQRIILADGSALEIAKQARQEGVRSLRRSGLHK
VKLGVTSLEEVLAVTNE

Nucleotide


Download         Length: 1734 bp        

>NTDB_id=230871 CCO03_RS15775 WP_236904156.1 3697782..3699515(+) (pilB) [Comamonas serinivorans strain DSM 26136]
ATGGAGTCCACCCTGGCCAGTAAGAGTTTTGCCACCGCCCTGCCGGGGCTGGGCCGCGCGCTGATTGCAGCAGGCAGGGT
GGACGAAGCGACGGCGGGCAAGGCCTTTGAGCAGGTCAAAGGCGACACCACGGCGTTTTTGCCCGAACTCATCAGTCGCG
GCGTGGTGACGGCTTCCGAGGTTGCGCACACCCTGGCCGACACCTTGGCCGCCCCGTTGCTGGATCTGGCTTCGCTGGAT
TACGACAGCCTGCCCAAAGACCTCATCGACGCCAAACTGGCTCGGCAGTATGCGGTGTTGCCGCTGGCCAAGAAGCGCGG
GCGGCTGGTCGTGGCCACCGCCGACCCCACCCTTCAGGAAGCGGCTGAGAAGATCAAGTTCACCACCCAGCTGGGGGTGG
AATGGGTGGTCGCTGAATACGACAAGCTGCAGGAAGCCATTGCCGCTGTCACCGGCTCGGCCAATGAGGCAGTCGGCAAC
TTCATCGCCACCGATTTCGGCTTCGACGATGCGTCTTTCGCCACCGATGGCGAAGACGACAGCGGCAAAGACGACACCAG
CGATGTGGAAGACGCGCCCATCGTGCGCTTTCTGCACAAGATGCTGGTCGACGCCTACAACATGCGGGCCTCCGACCTGC
ATTTCGAGCCATTCGAGCACAGCTACCGCGTGCGCTTCCGCATCGACGGCGAACTGCGCGAAATCACCTCGTTGCCGCCC
GGCATCAAAGAAAAGCTGGCGTCCCGCATCAAGGTGATTTCCCGCCTGGACATTTCGGAAAAGCGCGTTCCTCAAGACGG
GCGCATGAAGCTCAAGCTGGGTCCGGAACGCGTGATCGACTTCCGTGTGAGCACCCTGCCCACGCTGTTTGGCGAAAAAA
TCGTCATCCGGATCCTGGACCCCAGCAGCGCCAAGATGGGCATCGACGCCCTGGGCTATGAGCCGATTGAAAAAGAGCGC
CTGCTGGAGGCCATTGCCCGCCCCTACGGCATGATTCTGGTGACCGGCCCGACCGGCTCGGGCAAGACCGTGTCGCTCTA
CACCTGCCTGAACCTGCTGAACAAGCCCGGTGTCAACATCTCCACGGCAGAAGACCCGTCGGAAATCAACTTGCCCGGCG
TGAACCAGGTCAACGTCAACGAAAAGGCGGGGCTGACCTTCGCCACCGCCCTCAAGGCTTTCCTGCGCCAGGATCCCGAC
ATCATCATGGTGGGCGAAATCCGGGACCTGGAAACCGCCGACATCGCCATCAAGGCCGCTCAAACGGGTCACCTGGTGCT
GTCGACGCTGCACACCAACGACGCCCCGACCACCTTGACGCGCATGCGCAACATGGGCATTGCGCCCTTCAACATCGCAT
CCAGCGTCATCCTGATCACGGCGCAGCGGCTGGGGCGAAGGCTGTGCCCGGCCTGCAAGGAGCCCATCGACGTTCCGCGT
GACGCGCTGCTGGATGCCGGATTTGACGAAGAAGAACTGGACGGCAGCTGGGTGCCCTACCGCCCGGTGGGCTGCGGCAA
GTGCAACAACGGCTACAAGGGCCGGGTCGGCATCTATGAAGTCATGCCCATCACGGAAGACCTTCAACGCATCATCCTGG
CCGACGGGAGTGCCTTGGAAATCGCGAAGCAGGCGCGTCAGGAAGGCGTGCGCTCGCTTCGACGCTCTGGCTTGCACAAG
GTGAAGCTGGGTGTGACGTCGCTGGAAGAAGTCCTGGCCGTCACCAACGAATGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(50-153)

Type II/IV secretion system protein (T2SSE)

(200-467)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

54.657

98.614

0.539
  pilB Acinetobacter baylyi ADP1

54.657

98.614

0.539
  pilF Neisseria gonorrhoeae MS11

52.622

92.548

0.487
  pilB Legionella pneumophila strain ERS1305867

48.404

97.747

0.473
  pilB Vibrio cholerae strain A1552

48.429

93.761

0.454
  pilB Vibrio parahaemolyticus RIMD 2210633

47.978

94.281

0.452
  pilB Vibrio campbellii strain DS40M4

45.629

99.133

0.452
  pilF Thermus thermophilus HB27

38.144

100

0.385
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

41.199

92.548

0.381
  pilB/pilB1 Synechocystis sp. PCC 6803

35.948

100

0.381

Multiple sequence alignment