Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   CCK83_RS04735 Genome accession   NZ_CP021428
Coordinates   985252..986310 (-) Length   352 a.a.
NCBI ID   WP_032006368.1    Uniprot ID   -
Organism   Acinetobacter pittii strain HUMV-6483     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 980252..991310
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CCK83_RS04710 aroK 980554..981123 (-) 570 WP_002116987.1 shikimate kinase AroK -
  CCK83_RS04715 pilQ 981135..983261 (-) 2127 WP_017481729.1 type IV pilus secretin PilQ Machinery gene
  CCK83_RS04720 pilP 983324..983851 (-) 528 WP_002116864.1 pilus assembly protein PilP Machinery gene
  CCK83_RS04725 pilO 983862..984614 (-) 753 WP_032006367.1 type 4a pilus biogenesis protein PilO Machinery gene
  CCK83_RS04730 pilN 984611..985252 (-) 642 WP_002116944.1 PilN domain-containing protein Machinery gene
  CCK83_RS04735 pilM 985252..986310 (-) 1059 WP_032006368.1 pilus assembly protein PilM Machinery gene
  CCK83_RS04740 ponA 986472..989033 (+) 2562 WP_125518618.1 penicillin-binding protein PBP1a -
  CCK83_RS04745 rrmA 989048..989872 (-) 825 WP_087400197.1 putative RNA methyltransferase -
  CCK83_RS04750 - 989875..990207 (-) 333 WP_002116981.1 DUF7709 family protein -

Sequence

Protein


Download         Length: 352 a.a.        Molecular weight: 39015.60 Da        Isoelectric Point: 4.4213

>NTDB_id=230666 CCK83_RS04735 WP_032006368.1 985252..986310(-) (pilM) [Acinetobacter pittii strain HUMV-6483]
MLRLYRKPNKGLMGVDISSTSVKLLELSVKNGKYWVESYALTPLPENSVVEKNILNPEAVAEALERAMNLANPHTTNAAI
AVPTSTVIHKTIEMDADMNDDEREVQIRLDAEQYIPFPLDEVSLDFEVLPDRLPNPNRVNVLLVATRTENVETRVEVLEL
VDLTPKLADVESYAVERAFSVFADSLPMGANTIGILDIGHTMTTLSVMQNGKIIYTREQVFGGKQLTLEIQSRYGLSLEE
AGRAKKECSLPDDYDIEILEPFLDAVVQQAARSLQFFFSSSQFNEIDHILLAGGNANIPGLAKLLQQKLGYRVTIANPFL
QMGFSPQVDVQKIENDASSLMVACGLALRSFD

Nucleotide


Download         Length: 1059 bp        

>NTDB_id=230666 CCK83_RS04735 WP_032006368.1 985252..986310(-) (pilM) [Acinetobacter pittii strain HUMV-6483]
GTGCTCAGGTTATATCGTAAACCGAATAAGGGGTTAATGGGCGTCGATATTAGTTCGACTTCTGTTAAGTTGTTAGAGCT
CTCTGTTAAGAACGGTAAATATTGGGTAGAAAGCTATGCTTTAACGCCTTTACCTGAGAACAGTGTAGTTGAAAAAAATA
TCTTGAATCCAGAAGCAGTTGCAGAAGCTTTGGAACGGGCGATGAATTTAGCAAATCCCCACACCACTAATGCTGCAATT
GCTGTTCCAACATCGACGGTTATTCATAAAACTATCGAAATGGATGCAGATATGAACGATGATGAACGTGAAGTTCAGAT
TCGATTAGATGCGGAGCAATATATACCGTTCCCTTTAGATGAGGTGAGCCTTGATTTTGAGGTTTTACCAGATCGACTGC
CGAATCCAAATCGTGTGAATGTACTTTTAGTCGCAACACGAACAGAAAATGTTGAAACACGCGTAGAAGTACTGGAACTG
GTTGATTTAACGCCTAAGCTTGCTGATGTTGAAAGTTATGCTGTCGAACGTGCTTTTAGTGTGTTTGCTGATAGCTTACC
CATGGGTGCAAATACCATTGGTATTTTAGATATTGGCCATACCATGACCACATTATCGGTTATGCAAAATGGCAAAATCA
TTTATACCCGTGAACAAGTCTTTGGCGGTAAACAGCTTACTTTGGAAATTCAAAGCCGTTACGGTTTGTCATTAGAAGAA
GCGGGACGCGCTAAAAAAGAATGTTCTTTGCCAGATGATTATGACATCGAAATCTTAGAACCATTTTTAGATGCTGTAGT
TCAGCAAGCTGCTAGATCTCTACAATTTTTCTTCTCTTCATCCCAATTCAATGAAATTGACCATATTTTGCTAGCGGGTG
GCAATGCCAACATTCCAGGGCTTGCTAAATTGTTGCAGCAGAAACTCGGTTACCGAGTCACTATTGCCAATCCATTTTTA
CAAATGGGTTTCTCTCCTCAAGTCGACGTTCAAAAAATTGAAAATGATGCTTCTTCATTAATGGTGGCATGTGGCTTGGC
TTTAAGGAGTTTTGATTAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-351)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Acinetobacter baumannii D1279779

96.023

100

0.96
  comM Acinetobacter nosocomialis M2

96.023

100

0.96
  comM Acinetobacter baylyi ADP1

81.25

100

0.813
  pilM Legionella pneumophila strain ERS1305867

41.808

100

0.42

Multiple sequence alignment