Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   CCK83_RS04725 Genome accession   NZ_CP021428
Coordinates   983862..984614 (-) Length   250 a.a.
NCBI ID   WP_032006367.1    Uniprot ID   A0AAN4MGF9
Organism   Acinetobacter pittii strain HUMV-6483     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 978862..989614
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CCK83_RS04705 aroB 979443..980525 (-) 1083 WP_087400195.1 3-dehydroquinate synthase -
  CCK83_RS04710 aroK 980554..981123 (-) 570 WP_002116987.1 shikimate kinase AroK -
  CCK83_RS04715 pilQ 981135..983261 (-) 2127 WP_017481729.1 type IV pilus secretin PilQ Machinery gene
  CCK83_RS04720 pilP 983324..983851 (-) 528 WP_002116864.1 pilus assembly protein PilP Machinery gene
  CCK83_RS04725 pilO 983862..984614 (-) 753 WP_032006367.1 type 4a pilus biogenesis protein PilO Machinery gene
  CCK83_RS04730 pilN 984611..985252 (-) 642 WP_002116944.1 PilN domain-containing protein Machinery gene
  CCK83_RS04735 pilM 985252..986310 (-) 1059 WP_032006368.1 pilus assembly protein PilM Machinery gene
  CCK83_RS04740 ponA 986472..989033 (+) 2562 WP_125518618.1 penicillin-binding protein PBP1a -

Sequence

Protein


Download         Length: 250 a.a.        Molecular weight: 28225.10 Da        Isoelectric Point: 4.8782

>NTDB_id=230664 CCK83_RS04725 WP_032006367.1 983862..984614(-) (pilO) [Acinetobacter pittii strain HUMV-6483]
MSPDELHELSLEQAPSKKKKITLEKFLQQFNTLDMNSYGSWPLSVKITCWIFIFFAVLALGYFVAIQPKLQAIDNAQAQE
SNLLNEFREKDSKLRNLQQYQLQLQEMQANFNQQLEQLPKETEIPSLVEDINLTGVNSGLKFKNIRLEDEVKQEIFIEQP
ITIEATGDYHAFGAFVSSIAALPRIVTMHDFIVEAAPVKDGKSDIPVLNYSIKAKTYRYMGAAENPEHQEKNASASSVAP
DSSTANIQPK

Nucleotide


Download         Length: 753 bp        

>NTDB_id=230664 CCK83_RS04725 WP_032006367.1 983862..984614(-) (pilO) [Acinetobacter pittii strain HUMV-6483]
ATGAGTCCTGATGAACTTCATGAGCTGTCTTTAGAACAAGCTCCATCTAAGAAAAAAAAGATCACCTTAGAAAAATTCCT
TCAGCAATTTAACACGCTCGACATGAACAGTTATGGCAGCTGGCCATTATCTGTGAAAATTACGTGTTGGATTTTTATTT
TCTTTGCTGTACTGGCTCTAGGGTATTTTGTTGCTATTCAACCAAAGCTACAAGCAATTGATAATGCTCAAGCACAAGAA
AGTAATTTATTAAATGAGTTTCGAGAAAAAGACTCAAAATTACGCAACTTGCAGCAATACCAGCTTCAACTTCAAGAGAT
GCAAGCCAACTTCAATCAGCAGTTAGAACAATTGCCAAAAGAAACCGAAATTCCAAGTTTAGTTGAAGATATTAACTTAA
CGGGGGTGAATTCTGGTCTGAAGTTTAAAAATATCCGCTTAGAAGATGAAGTGAAGCAGGAAATTTTTATTGAACAGCCT
ATTACTATCGAAGCAACTGGGGATTATCACGCTTTCGGTGCTTTTGTTAGCAGTATTGCGGCATTACCACGTATTGTGAC
GATGCATGATTTTATTGTTGAAGCTGCTCCTGTTAAGGATGGTAAATCAGATATTCCTGTGCTTAATTATTCTATCAAAG
CAAAAACTTATCGCTATATGGGTGCTGCAGAAAATCCTGAACATCAGGAAAAAAATGCTTCGGCTTCCTCGGTAGCACCT
GATTCTTCTACTGCTAACATACAGCCGAAATAG

Domains

Predicted by InterproScan.

Pilus assembly protein, PilO (PilO)

(73-220)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Acinetobacter baumannii D1279779

83.6

100

0.836
  comO Acinetobacter baylyi ADP1

69.583

96

0.668

Multiple sequence alignment