Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   OLMES_RS03860 Genome accession   NZ_CP021425
Coordinates   851206..853368 (+) Length   720 a.a.
NCBI ID   WP_232465254.1    Uniprot ID   -
Organism   Oleiphilus messinensis strain ME102     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 846206..858368
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OLMES_RS03840 (OLMES_0785) pilM 848392..849456 (+) 1065 WP_087460039.1 pilus assembly protein PilM Machinery gene
  OLMES_RS03845 (OLMES_0786) - 849458..850027 (+) 570 WP_087460040.1 PilN domain-containing protein -
  OLMES_RS03850 (OLMES_0787) - 850024..850638 (+) 615 WP_087460041.1 type 4a pilus biogenesis protein PilO -
  OLMES_RS03855 (OLMES_0788) - 850644..851192 (+) 549 WP_087460042.1 pilus assembly protein PilP -
  OLMES_RS03860 (OLMES_0789) pilQ 851206..853368 (+) 2163 WP_232465254.1 type IV pilus secretin PilQ Machinery gene
  OLMES_RS03865 (OLMES_0790) aroK 853557..854099 (+) 543 WP_087460043.1 shikimate kinase AroK -
  OLMES_RS03870 (OLMES_0791) aroB 854180..855262 (+) 1083 WP_087460044.1 3-dehydroquinate synthase -
  OLMES_RS03875 (OLMES_0792) - 855311..857044 (+) 1734 WP_198343209.1 AAA family ATPase -

Sequence

Protein


Download         Length: 720 a.a.        Molecular weight: 78113.81 Da        Isoelectric Point: 4.7362

>NTDB_id=230574 OLMES_RS03860 WP_232465254.1 851206..853368(+) (pilQ) [Oleiphilus messinensis strain ME102]
MRLILNNYLQYGLVSKMFFNAKNAVWTSLIAVLFFPLSVVGATLNDVSFASLPGDKTEIRFSFDQTPPMPKGYTIERPAR
IALDIPDVSSALKSKYHNLGVGNAQSLTVIEAKDRVRVIINLVELVPYSSVRHGNELVVVVGSGSLMSPSSGEHAEGASS
VSGQQPGVFVSTSAVAQANQEPALESIDFKRGEKGDGQVVLSLNTASVDVDMSEQAGNIRLEFKGVALPAELKRRFDVTD
FATAVTRIDALSEEGNAVILIRPEGNYDFLAYQADNQFTVSVEALTSEEVEQRRKEKFPYSGEKLSLNFQDIDVRAVLQL
IADFTGLNLVASDTVSGKITLRLQNVPWDQALELVLKTKGLDKRKVGNVLLVAPAAEIAAREKLELETSKQVKELAPVRL
EIVQVNYAKAADIVRLLRADKELISDRGFISSDTRTNSVSVRETSDKLDQIRRLVAKWDVPVRQVLIEARIVRARSNLTQ
DLGIQWGGGYLTQKGDSLYRVGGSSSTLSELTEVAAGNSNTITFPDALAVDLGVTRVNTSSIALGYGNDNMLIDMELSAF
ETDGKGEIVAQPKIVTADRKKAIIKSGEQIPYQEASSSGATSVSFKEAALSLEVTPQITPDDKIIMDLKINQDARGEVTG
GIPSIDTNEIETQVLVGNGETIVLGGIFQAETSTTVTKTPFLGDLPYIGSLFKKTEEIDERGELLIFITPKLLKGGLTDQ

Nucleotide


Download         Length: 2163 bp        

>NTDB_id=230574 OLMES_RS03860 WP_232465254.1 851206..853368(+) (pilQ) [Oleiphilus messinensis strain ME102]
ATGAGATTGATACTAAACAACTATCTGCAATATGGGCTGGTGTCTAAAATGTTTTTTAATGCTAAAAATGCAGTGTGGAC
GAGCTTGATAGCAGTTTTATTTTTTCCTTTGAGCGTGGTTGGTGCGACATTAAATGATGTCAGCTTTGCATCACTGCCGG
GAGATAAAACTGAAATCAGGTTTTCATTCGATCAAACGCCTCCTATGCCTAAAGGATACACTATCGAAAGGCCTGCCCGA
ATTGCTCTCGATATTCCGGATGTATCGAGCGCACTGAAGAGCAAGTATCATAATCTGGGAGTCGGAAATGCACAAAGTCT
AACCGTTATAGAGGCAAAGGACAGGGTGCGGGTTATTATAAACTTGGTCGAGTTAGTGCCTTATTCGTCCGTTAGGCACG
GCAATGAACTTGTTGTTGTTGTTGGGTCTGGTAGTTTAATGTCTCCGTCTTCGGGAGAGCATGCGGAGGGGGCATCCTCG
GTATCGGGTCAGCAGCCCGGGGTATTTGTCAGTACGTCTGCGGTTGCTCAAGCCAATCAGGAGCCTGCCCTCGAAAGTAT
AGATTTCAAGCGGGGTGAGAAAGGGGATGGCCAGGTTGTCTTGTCTTTGAATACGGCCAGTGTTGATGTGGATATGTCGG
AACAAGCAGGAAATATCCGTCTCGAATTCAAAGGTGTAGCGTTGCCAGCGGAATTGAAAAGGCGCTTTGATGTGACTGAC
TTCGCCACGGCAGTTACGCGAATTGATGCGCTGAGTGAAGAGGGAAATGCAGTGATTTTGATCCGTCCTGAGGGAAATTA
TGATTTTCTGGCTTATCAAGCAGACAATCAGTTCACAGTCAGTGTCGAAGCCCTGACATCTGAAGAGGTGGAGCAGCGCA
GGAAAGAGAAATTCCCTTACTCCGGTGAAAAATTATCATTGAATTTTCAAGATATTGATGTTCGAGCAGTGCTGCAGTTA
ATAGCTGACTTTACAGGTTTGAATCTCGTTGCAAGCGATACTGTTTCTGGAAAAATTACCCTTAGACTGCAAAATGTACC
GTGGGATCAAGCCCTGGAATTGGTTTTGAAAACCAAAGGATTAGACAAGCGTAAAGTTGGCAACGTGCTATTGGTGGCGC
CGGCTGCTGAAATTGCTGCCCGTGAGAAGCTTGAGTTGGAAACGTCCAAGCAGGTCAAAGAGTTGGCACCAGTTCGTTTG
GAAATTGTTCAGGTGAACTACGCTAAAGCTGCAGACATTGTAAGGTTACTACGTGCGGATAAGGAGTTGATTTCTGATCG
AGGATTTATTTCTTCAGACACTCGAACCAATAGTGTGAGTGTTCGAGAAACCTCTGACAAACTAGATCAAATTCGCAGAT
TGGTTGCAAAGTGGGATGTGCCCGTGCGACAGGTGTTAATAGAAGCTCGAATTGTAAGAGCTCGTAGCAACCTCACTCAA
GACTTGGGGATTCAATGGGGTGGGGGGTATTTGACACAAAAAGGAGACAGCCTTTATCGTGTTGGTGGGTCGTCTTCAAC
ATTGTCGGAGTTGACAGAAGTTGCTGCCGGCAATTCTAATACAATTACGTTTCCAGATGCATTGGCAGTTGATTTGGGGG
TTACCCGGGTAAATACGTCTTCAATTGCATTGGGGTATGGCAATGACAATATGCTCATTGATATGGAGCTTTCAGCGTTC
GAAACGGATGGGAAGGGAGAGATAGTCGCACAGCCAAAAATAGTAACTGCTGATCGAAAAAAGGCCATAATAAAATCGGG
TGAACAAATCCCATATCAGGAGGCGTCTTCCAGTGGTGCTACTTCAGTATCGTTCAAAGAGGCCGCATTATCTCTGGAGG
TGACTCCCCAAATTACACCTGATGATAAAATTATTATGGATCTAAAGATTAATCAGGATGCTCGGGGGGAGGTAACCGGC
GGAATTCCGAGCATTGATACGAATGAGATTGAAACGCAAGTACTGGTTGGTAATGGTGAAACGATAGTCTTGGGAGGTAT
ATTTCAGGCGGAAACCTCAACAACGGTGACAAAAACACCGTTTTTAGGTGATCTCCCATATATTGGTTCCTTGTTTAAGA
AAACGGAAGAGATTGATGAGCGCGGAGAATTGTTGATTTTTATTACCCCCAAGTTGTTGAAAGGTGGGTTGACAGATCAG
TAG

Domains

Predicted by InterproScan.

AMIN domain (AMIN)

(55-139)

Secretin and TonB N terminus short domain (STN)

(328-374)

Bacterial type II and III secretion system protein (Secretin)

(559-713)

Bacterial type II/III secretion system short domain (Secretin_N)

(401-464)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

50.282

98.333

0.494
  comQ Acinetobacter baylyi ADP1

40.837

96.25

0.393
  pilQ Acinetobacter baumannii strain A118

38.095

100

0.389
  pilQ Acinetobacter baumannii D1279779

38.095

100

0.389
  pilQ Legionella pneumophila strain ERS1305867

39.478

95.694

0.378
  pilQ Legionella pneumophila strain Lp02

39.478

95.694

0.378

Multiple sequence alignment