Detailed information NTG0230573

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: OLMES_RS03840
• Genome accession: NZ_CP021425
• Coordinates: 848392..849456 (+)
• Length: 354 a.a.
• NCBI ID: WP_087460039.1
• Uniprot ID: A0A1Y0I344
• Organism: Oleiphilus messinensis strain ME102
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 843392..854456

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
OLMES_RS03825	rpmE	843760..843978 (+)	219	WP_087460036.1	50S ribosomal protein L31	-
OLMES_RS03830 (OLMES_0783)	-	844237..845502 (+)	1266	WP_087460037.1	malic enzyme-like NAD(P)-binding protein	-
OLMES_RS03835 (OLMES_0784)	-	845618..848197 (-)	2580	WP_087460038.1	penicillin-binding protein 1A	-
OLMES_RS03840 (OLMES_0785)	pilM	848392..849456 (+)	1065	WP_087460039.1	pilus assembly protein PilM	Machinery gene
OLMES_RS03845 (OLMES_0786)	-	849458..850027 (+)	570	WP_087460040.1	PilN domain-containing protein	-
OLMES_RS03850 (OLMES_0787)	-	850024..850638 (+)	615	WP_087460041.1	type 4a pilus biogenesis protein PilO	-
OLMES_RS03855 (OLMES_0788)	-	850644..851192 (+)	549	WP_087460042.1	pilus assembly protein PilP	-
OLMES_RS03860 (OLMES_0789)	pilQ	851206..853368 (+)	2163	WP_232465254.1	type IV pilus secretin PilQ	Machinery gene
OLMES_RS03865 (OLMES_0790)	aroK	853557..854099 (+)	543	WP_087460043.1	shikimate kinase AroK	-

Sequence

Protein

• Length: 354 a.a.
• Molecular weight: 38097.07 Da
• Isoelectric Point: 4.1156

>NTDB_id=230573 OLMES_RS03840 WP_087460039.1 848392..849456(+) (pilM) [Oleiphilus messinensis strain ME102]
MFGLFGSKNSVLLGIDISSTSVKLLELSKSGGRCKVESYAVEPLPPNAVVEKNISDIELVGEAIRKVVAKARTNNKFSAV
AVAGSAVITKTIQMAGGLNDQEMESQISVEADQYIPYPLDEVAIDFEVQGESDQNPEQVDVLLAACRRENVELREDALEI
AGLTTRIVDVEAYAMERAFGLIEPHLDAGAEEQTVAIVDIGATMTTLSVLVDGQTIYTREQLFGGKQLTEEIQRRYGLSQ
EEAGLAKKQGGLPDDYEDEVLQPFKEAVVQQVARSLQFFFSSSQYNEVDYVILAGGTASISGIAELVQEKVATPTLVANP
FADMALSSKVNASALSNDAPSLMIACGLAMRSFD

Nucleotide

• Length: 1065 bp

>NTDB_id=230573 OLMES_RS03840 WP_087460039.1 848392..849456(+) (pilM) [Oleiphilus messinensis strain ME102]
GTGTTTGGTTTATTTGGAAGCAAAAACTCAGTCCTATTGGGAATTGATATCAGCTCCACGTCCGTGAAGTTATTGGAGCT
CAGTAAAAGCGGTGGTCGTTGCAAAGTCGAAAGTTACGCCGTTGAACCCCTCCCGCCTAACGCTGTTGTCGAGAAAAATA
TCAGCGATATCGAACTTGTAGGTGAGGCTATCAGAAAAGTGGTGGCGAAAGCCAGGACTAATAATAAGTTTTCTGCTGTC
GCTGTTGCAGGGTCTGCCGTAATTACGAAAACAATCCAGATGGCCGGTGGATTGAATGATCAGGAGATGGAAAGCCAGAT
ATCTGTCGAAGCGGATCAATATATCCCTTACCCACTCGACGAAGTAGCAATTGATTTCGAAGTTCAGGGTGAGTCGGATC
AGAATCCCGAGCAGGTTGATGTTTTGCTGGCGGCGTGTCGGCGTGAGAATGTTGAGTTGCGAGAGGACGCCTTGGAAATA
GCCGGGTTGACGACTCGCATTGTCGATGTGGAAGCTTATGCGATGGAGCGGGCTTTTGGTTTGATCGAGCCTCATCTGGA
TGCGGGGGCGGAGGAGCAAACGGTTGCCATCGTCGATATCGGGGCAACAATGACAACGCTGAGCGTGTTGGTCGATGGTC
AAACGATTTATACGCGCGAGCAATTGTTTGGCGGCAAGCAACTGACAGAAGAAATTCAACGCCGTTATGGTCTGTCTCAG
GAAGAGGCTGGACTGGCTAAAAAACAAGGCGGGCTGCCTGACGACTATGAAGACGAAGTCTTGCAACCCTTTAAAGAAGC
TGTTGTTCAACAGGTTGCTCGATCGCTTCAGTTCTTTTTCTCCTCCAGTCAGTATAACGAAGTCGATTATGTGATTTTGG
CCGGAGGGACTGCATCCATCTCGGGAATTGCTGAACTAGTTCAGGAGAAAGTTGCGACGCCGACTCTCGTCGCCAACCCT
TTTGCTGACATGGCATTGAGTTCGAAAGTTAATGCTTCTGCGTTAAGCAACGACGCACCTTCTTTGATGATCGCTTGTGG
GTTGGCAATGAGGAGTTTTGATTGA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (14-353)

3D structure

• AlphaFold DB: A0A1Y0I344

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Acinetobacter baumannii D1279779	57.062	100	0.571
comM	Acinetobacter nosocomialis M2	57.062	100	0.571
comM	Acinetobacter baylyi ADP1	53.672	100	0.537
pilM	Legionella pneumophila strain ERS1305867	46.328	100	0.463