Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   CBR65_RS16330 Genome accession   NZ_CP021382
Coordinates   3914839..3916884 (+) Length   681 a.a.
NCBI ID   WP_087469121.1    Uniprot ID   A0A1Y0G2Y1
Organism   Cellvibrio sp. PSBB006     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3909839..3921884
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CBR65_RS16310 (CBR65_16290) pilM 3912005..3913072 (+) 1068 WP_087467838.1 pilus assembly protein PilM Machinery gene
  CBR65_RS16315 (CBR65_16295) - 3913072..3913653 (+) 582 WP_087467839.1 PilN domain-containing protein -
  CBR65_RS16320 (CBR65_16300) - 3913655..3914266 (+) 612 WP_157672116.1 type 4a pilus biogenesis protein PilO -
  CBR65_RS16325 (CBR65_16305) - 3914269..3914802 (+) 534 WP_087467840.1 pilus assembly protein PilP -
  CBR65_RS16330 (CBR65_16310) pilQ 3914839..3916884 (+) 2046 WP_087469121.1 type IV pilus secretin PilQ Machinery gene
  CBR65_RS16335 (CBR65_16315) aroK 3916884..3917402 (+) 519 WP_087467841.1 shikimate kinase AroK -
  CBR65_RS16340 (CBR65_16320) aroB 3917473..3918549 (+) 1077 WP_087467842.1 3-dehydroquinate synthase -
  CBR65_RS16345 (CBR65_16325) - 3918600..3920189 (+) 1590 WP_087467843.1 SPOR domain-containing protein -

Sequence

Protein


Download         Length: 681 a.a.        Molecular weight: 73994.77 Da        Isoelectric Point: 4.3742

>NTDB_id=230331 CBR65_RS16330 WP_087469121.1 3914839..3916884(+) (pilQ) [Cellvibrio sp. PSBB006]
MRNIALLLVCLLAPVTWANTLDDIRFAELPGERFEVRMTFSGPPPEPAGYTIERPARIVLDFPQVVSALEERRYPLSFDN
GQSAMVLTTEGRTRLILNLDDLTSYTSRAEGNTYIVEVGASDGGYANNTVTPSVTVPSAPTAQTAAAQGPAITNIDFRRG
TTGEGRIIIALSDPGISAEMSSTGAGVRLTFNDVWLPPELRRRLDVVDFATPVSLVSATLDGGDTVLNIAASGDYDYLAY
QADKEYVVSIKPLTAQEIKEKKEEFEFTGEKLSLNFQDIEVRAVLQIIADFTELNLVASDTVQGRITLRLDNVPWDQALS
LVLKTKGLDKRQVGNVLMVAPAAEIAERERQELTTKKQLEELAPLRTEYIRVRYANAKEMFELFRGESGERSGGSGAGRG
GSQSTGSVLSERGQAIVDERTNSIIITDTADRIEAFRRLIDQIDIPIRQVMIEARIVIANTDFRRELGIRWGAGGTDQSS
DTVTDVEGSVDLGVTTSPAGTLALGVLTDNEFLDMELSALENTGFAEIVSQPKVITGDKQRATISSGTEIPYQEASASGA
TTTSFKEAVLKLDVTPQITPDNRIIMDLVINQDTVGQIIVASGIPTLDVTELTTKVLVANGQTVVLGGVFTVESIKGENK
VPLLGDIPYLGRLFRRDVNENNKTELLLFITPKIMAESLTN

Nucleotide


Download         Length: 2046 bp        

>NTDB_id=230331 CBR65_RS16330 WP_087469121.1 3914839..3916884(+) (pilQ) [Cellvibrio sp. PSBB006]
ATGCGAAATATAGCCTTATTACTTGTTTGTCTGTTGGCCCCTGTCACCTGGGCAAATACACTTGATGATATTCGTTTTGC
TGAACTTCCCGGTGAGCGGTTTGAAGTCAGGATGACGTTTTCAGGGCCGCCACCGGAACCTGCCGGTTACACTATTGAAA
GGCCTGCGCGCATTGTCCTGGACTTTCCTCAGGTAGTCAGTGCGCTGGAAGAGCGTCGTTATCCTTTATCGTTTGACAAT
GGTCAGAGTGCCATGGTGTTAACGACGGAGGGTAGAACCCGGTTGATTTTAAATCTGGATGATTTGACGTCTTACACCAG
TCGTGCCGAAGGTAATACCTACATTGTTGAAGTGGGTGCTAGTGACGGTGGTTATGCAAATAACACGGTGACTCCCAGCG
TTACGGTTCCATCGGCACCAACAGCTCAAACCGCTGCAGCTCAAGGGCCTGCAATCACCAATATTGACTTCCGTCGTGGC
ACTACAGGTGAGGGTCGTATCATTATTGCGCTGTCAGATCCTGGTATAAGTGCCGAGATGTCGAGCACTGGCGCAGGGGT
TCGGTTAACCTTTAACGATGTCTGGCTGCCGCCGGAATTGCGCCGCCGACTGGACGTAGTGGATTTCGCGACGCCGGTTT
CATTGGTATCGGCGACACTTGATGGTGGCGACACCGTACTGAATATAGCTGCGTCGGGTGACTACGATTATCTGGCCTAC
CAAGCGGACAAAGAATATGTCGTTAGCATTAAGCCGCTGACGGCTCAGGAAATTAAAGAGAAAAAAGAAGAATTTGAATT
TACCGGTGAAAAGCTGTCACTGAACTTCCAGGATATTGAAGTGCGTGCGGTGCTGCAAATCATTGCCGACTTTACTGAAT
TGAATCTTGTAGCCAGTGACACTGTGCAAGGCCGTATTACTTTGCGTCTTGATAACGTGCCTTGGGATCAAGCGCTCAGC
CTGGTATTAAAAACCAAAGGGCTGGATAAGCGTCAGGTGGGTAATGTTCTAATGGTTGCGCCTGCTGCGGAAATTGCCGA
ACGTGAACGTCAGGAACTGACTACCAAGAAACAATTGGAAGAATTAGCACCATTGCGCACGGAATATATTCGTGTTCGTT
ATGCTAATGCTAAAGAGATGTTTGAGTTGTTCCGTGGTGAGTCTGGTGAGCGGTCTGGTGGAAGTGGGGCCGGTAGAGGC
GGTAGCCAATCTACCGGCAGTGTTCTCTCTGAGCGTGGACAAGCGATTGTTGATGAGCGAACTAACTCTATCATTATTAC
TGATACCGCCGACCGTATCGAAGCCTTCAGGCGATTGATCGATCAAATTGATATCCCGATTCGCCAAGTGATGATTGAAG
CGCGGATTGTGATTGCTAACACCGATTTCCGTAGAGAGTTGGGTATCCGTTGGGGGGCGGGTGGTACTGATCAGTCTTCT
GATACGGTTACCGACGTTGAAGGTTCGGTGGATTTAGGTGTGACGACCAGTCCTGCTGGTACTCTTGCGCTTGGTGTTCT
GACTGATAATGAGTTTCTAGACATGGAATTGTCAGCACTTGAGAATACCGGTTTTGCTGAAATAGTCTCTCAGCCGAAAG
TTATCACTGGCGATAAACAGCGTGCCACTATTTCTTCAGGCACTGAAATCCCTTATCAAGAAGCTTCTGCCAGTGGCGCT
ACCACTACGTCATTCAAGGAAGCAGTTCTGAAGCTGGATGTGACTCCCCAAATTACGCCGGATAATCGCATTATTATGGA
CTTGGTGATAAATCAGGACACGGTTGGGCAAATTATCGTGGCCAGCGGGATTCCCACTCTTGATGTGACAGAGTTGACCA
CGAAAGTCTTGGTGGCCAATGGGCAAACCGTAGTATTGGGTGGTGTATTCACCGTGGAATCTATCAAGGGTGAGAACAAG
GTACCTCTCCTCGGTGATATCCCGTACCTGGGTCGTTTGTTCAGACGCGACGTTAATGAAAATAATAAAACAGAATTGCT
GTTGTTCATCACCCCCAAAATCATGGCTGAAAGCCTGACAAACTGA

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(368-449)

Secretin and TonB N terminus short domain (STN)

(295-341)

Bacterial type II and III secretion system protein (Secretin)

(519-675)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1Y0G2Y1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

49.646

100

0.515
  comQ Acinetobacter baylyi ADP1

41.364

100

0.418
  pilQ Acinetobacter baumannii strain A118

39.306

100

0.416
  pilQ Acinetobacter baumannii D1279779

39.306

100

0.416
  pilQ Legionella pneumophila strain ERS1305867

39.368

100

0.402
  pilQ Legionella pneumophila strain Lp02

39.368

100

0.402

Multiple sequence alignment