Detailed information NTG0230330

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: CBR65_RS16310
• Genome accession: NZ_CP021382
• Coordinates: 3912005..3913072 (+)
• Length: 355 a.a.
• NCBI ID: WP_087467838.1
• Uniprot ID: A0A1Y0G0L4
• Organism: Cellvibrio sp. PSBB006
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3907005..3918072

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
CBR65_RS16295 (CBR65_16275)	rpmE	3908243..3908461 (+)	219	WP_087469119.1	50S ribosomal protein L31	-
CBR65_RS16300 (CBR65_16280)	-	3908502..3909314 (+)	813	WP_157672115.1	thermonuclease family protein	-
CBR65_RS16305 (CBR65_16285)	-	3909370..3911850 (-)	2481	WP_232461227.1	penicillin-binding protein 1A	-
CBR65_RS16310 (CBR65_16290)	pilM	3912005..3913072 (+)	1068	WP_087467838.1	pilus assembly protein PilM	Machinery gene
CBR65_RS16315 (CBR65_16295)	-	3913072..3913653 (+)	582	WP_087467839.1	PilN domain-containing protein	-
CBR65_RS16320 (CBR65_16300)	-	3913655..3914266 (+)	612	WP_157672116.1	type 4a pilus biogenesis protein PilO	-
CBR65_RS16325 (CBR65_16305)	-	3914269..3914802 (+)	534	WP_087467840.1	pilus assembly protein PilP	-
CBR65_RS16330 (CBR65_16310)	pilQ	3914839..3916884 (+)	2046	WP_087469121.1	type IV pilus secretin PilQ	Machinery gene
CBR65_RS16335 (CBR65_16315)	aroK	3916884..3917402 (+)	519	WP_087467841.1	shikimate kinase AroK	-

Sequence

Protein

• Length: 355 a.a.
• Molecular weight: 38507.93 Da
• Isoelectric Point: 4.2891

>NTDB_id=230330 CBR65_RS16310 WP_087467838.1 3912005..3913072(+) (pilM) [Cellvibrio sp. PSBB006]
MGILGFLEKKAKPVLGLDISSTSVKLLELSRHGDRYRVETYAVRALPPNAVVEKNINDQEAVAEVIKAMVKQSKTKLKHA
AVAVAGSAVITKVIDMPAGLSDDAMETQISLEADQYIPFPLEEVAIDFEVQGESPRNPDQVEVLLAACRRENVEMRANVL
ELADLTPEKVDVEAYSMERAYELIADQLEDQDGQVVAIIDVGATMTTLSVLVDGKTVYTREQLFGGKQLTEEIQRRYGLS
MEEAGLAKKQGGLPDDYESEVLGPFKEAVVQQVTRSLQFFFSASQYNDVDYIILAGGVASMDGLVGLIEEKLGTQTVVAN
PFAGMSVSSRVNAVALANDAPSLMIATGLAMRSFD

Nucleotide

• Length: 1068 bp

>NTDB_id=230330 CBR65_RS16310 WP_087467838.1 3912005..3913072(+) (pilM) [Cellvibrio sp. PSBB006]
ATGGGCATCTTAGGCTTCCTCGAGAAAAAAGCGAAACCAGTGTTGGGACTGGATATCAGTTCAACTTCGGTCAAATTGCT
TGAATTAAGCCGCCACGGTGATCGCTATCGTGTCGAGACTTATGCTGTGAGAGCTCTACCGCCAAATGCGGTGGTGGAGA
AAAATATTAATGACCAGGAGGCCGTTGCTGAGGTCATCAAAGCAATGGTCAAGCAATCCAAGACCAAGCTGAAACATGCA
GCAGTTGCGGTTGCCGGTTCGGCGGTGATCACCAAAGTGATCGATATGCCTGCCGGGTTAAGTGACGATGCAATGGAAAC
CCAGATTTCCCTTGAAGCTGACCAATACATTCCCTTCCCTCTTGAAGAGGTGGCTATCGACTTCGAAGTTCAGGGCGAGT
CACCGCGCAATCCCGATCAGGTTGAGGTCTTGCTTGCCGCCTGTCGTCGTGAAAATGTTGAGATGCGCGCTAACGTCCTT
GAACTGGCTGATTTAACTCCGGAAAAAGTGGATGTTGAAGCCTACAGCATGGAGCGTGCCTACGAATTGATTGCGGACCA
GTTGGAAGATCAGGATGGTCAGGTTGTGGCTATCATTGACGTGGGCGCCACTATGACCACGCTCAGCGTCCTTGTCGATG
GCAAAACGGTTTATACCCGCGAACAACTGTTCGGTGGCAAGCAACTTACCGAAGAAATTCAACGCCGCTATGGTTTATCG
ATGGAAGAGGCCGGGTTGGCCAAGAAGCAAGGCGGTTTGCCAGACGATTATGAATCTGAAGTACTGGGCCCTTTTAAAGA
AGCTGTCGTGCAGCAGGTCACTCGTTCCCTACAATTTTTCTTCTCTGCCAGCCAATACAATGATGTCGATTACATTATTT
TGGCTGGTGGTGTGGCGTCGATGGACGGCCTGGTAGGTTTGATTGAAGAAAAATTGGGCACCCAGACTGTGGTAGCCAAT
CCCTTTGCCGGTATGTCGGTGTCGTCGCGGGTCAATGCTGTAGCGCTGGCTAATGATGCTCCTTCATTGATGATTGCCAC
TGGCTTGGCAATGAGGAGTTTCGACTAA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (16-354)

3D structure

• AlphaFold DB: A0A1Y0G0L4

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Acinetobacter baumannii D1279779	56.091	99.437	0.558
comM	Acinetobacter nosocomialis M2	55.807	99.437	0.555
comM	Acinetobacter baylyi ADP1	53.258	99.437	0.53
pilM	Legionella pneumophila strain ERS1305867	44.789	100	0.448