Detailed information NTG0229375

Bioinformatically predicted

Overview

• Name: pilQ
• Type: Machinery gene
• Locus tag: CA925_RS04735
• Genome accession: NZ_CP021266
• Coordinates: 1025406..1027502 (+)
• Length: 698 a.a.
• NCBI ID: WP_014843602.1
• Uniprot ID: Q6VY32
• Organism: Legionella pneumophila subsp. pneumophila strain Knoxville
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1020406..1032502

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
CA925_RS04710	-	1021171..1022178 (-)	1008	WP_014843598.1	hypothetical protein	-
CA925_RS04715	pilM	1022595..1023659 (+)	1065	WP_014843599.1	type IV pilus assembly protein PilM	Machinery gene
CA925_RS04720	pilN	1023663..1024211 (+)	549	WP_011213354.1	PilN domain-containing protein	Machinery gene
CA925_RS04725	pilO	1024225..1024821 (+)	597	WP_027224065.1	type 4a pilus biogenesis protein PilO	Machinery gene
CA925_RS04730	pilP	1024818..1025402 (+)	585	WP_014843601.1	pilus assembly protein PilP	Machinery gene
CA925_RS04735	pilQ	1025406..1027502 (+)	2097	WP_014843602.1	type IV pilus secretin PilQ	Machinery gene
CA925_RS04740	aroK	1027947..1028474 (+)	528	WP_010946667.1	shikimate kinase AroK	-
CA925_RS04745	aroB	1028461..1029570 (+)	1110	WP_013101269.1	3-dehydroquinate synthase	-
CA925_RS04750	-	1029567..1031018 (+)	1452	WP_027219627.1	SPOR domain-containing protein	-
CA925_RS04755	-	1031127..1031558 (+)	432	WP_010946670.1	universal stress protein	-

Sequence

Protein

• Length: 698 a.a.
• Molecular weight: 77441.66 Da
• Isoelectric Point: 9.4307

>NTDB_id=229375 CA925_RS04735 WP_014843602.1 1025406..1027502(+) (pilQ) [Legionella pneumophila subsp. pneumophila strain Knoxville]
MRKIIAILILISASLSLAYSKDNSLISVKVLPLPENRVRIDFQFSNPLKQLPASFITQKPPRLVLDFISTDMQIPSDQKT
KTIQLGSLHSYNIVAVGDRVRAILELDRSVSYSGSTAGSVYSLVLNGKSNDLFENSKEVFITNQVVNAKHEIKRIDFRGI
EKQGGRVLIDVSDTGIPIDVTQVGKEIVVNFLNTKIPLNLMKRYDVSDFHSPVQIITMQQEGKKVRMTILSKGDYGHFVY
QVNKQFMVDVFPLTAEEIRQAKLKKQVFTGKRISLNFQNISIRAVLQLLADFTGINMVVSDKVQGDITLRLNDIPWDQAL
DIILTTQGLDKRRKGNVMLIDTKASMDKMEEEQLKSQQTIEKLEPIRSDLIQINYAKAADIAVLIKDKQNSLLSDRGKIS
VDARTNTIWIQDTGTKIEEVRELIKQLDIPVKQVLIEARIVEVTKDFSQDIGIRWGVSRPTHLSGTLEGANELARGTAPA
NVVPLERRLNLDLAAAPLTGATPASVGIALAKLNDNILLDLELSALESEGRAELISSPRLITTNQQAAVIESGEEIPYQE
ATSSGATAVAFKKAVLSLKVTPQITPDGKILMDLQINQDTPSPQTFLGVPAIITKEIQTNVLVNNGQTIVLGGIYKQDKN
KVINRIPFFGELPVVGILFSNRQITLKNEELLIFITPRIITNALSITTIEGREKDVYK

Nucleotide

• Length: 2097 bp

>NTDB_id=229375 CA925_RS04735 WP_014843602.1 1025406..1027502(+) (pilQ) [Legionella pneumophila subsp. pneumophila strain Knoxville]
GTGAGAAAAATTATCGCGATTTTAATATTAATTAGTGCCAGTTTGAGTCTGGCATATTCTAAAGATAATTCGCTCATTTC
AGTAAAAGTATTGCCATTGCCAGAAAACAGAGTACGTATTGATTTTCAATTTTCCAACCCCTTAAAGCAGTTGCCCGCAA
GCTTTATTACTCAAAAGCCGCCTCGTTTGGTGTTAGATTTTATCAGCACTGATATGCAAATTCCTTCGGATCAGAAAACT
AAAACTATTCAATTAGGCTCATTACACAGTTATAACATTGTTGCTGTTGGTGATAGAGTAAGAGCCATACTGGAACTGGA
TCGTTCGGTGTCCTATTCTGGCAGTACGGCGGGCTCCGTTTATAGCCTTGTCCTGAATGGTAAAAGTAATGATTTATTTG
AAAATAGCAAGGAGGTATTCATAACGAACCAGGTTGTGAACGCCAAGCATGAAATTAAAAGAATTGATTTTCGTGGCATA
GAAAAGCAGGGAGGTAGAGTTTTAATTGATGTTTCTGATACGGGTATTCCAATTGATGTAACTCAAGTTGGGAAAGAAAT
AGTCGTTAATTTCCTGAATACCAAGATCCCTTTGAATTTAATGAAACGGTATGATGTTTCTGATTTTCACAGCCCTGTGC
AGATTATTACGATGCAACAGGAAGGCAAAAAGGTTCGCATGACCATCCTCAGCAAAGGGGATTATGGGCATTTTGTTTAT
CAGGTCAATAAACAATTTATGGTCGATGTCTTTCCTTTAACTGCGGAAGAAATTCGTCAGGCGAAATTGAAGAAACAGGT
ATTTACCGGTAAACGGATTTCTTTGAATTTCCAGAATATTAGTATTCGAGCTGTGCTTCAACTACTGGCAGATTTTACCG
GAATCAATATGGTTGTCAGTGACAAAGTGCAAGGAGATATTACGCTTAGATTAAATGATATCCCCTGGGATCAGGCTCTG
GATATCATTTTAACCACACAAGGGCTAGATAAGCGCAGAAAGGGCAACGTCATGTTGATAGATACCAAAGCCTCCATGGA
TAAAATGGAGGAAGAACAACTCAAAAGTCAACAGACAATTGAAAAACTTGAGCCTATCCGCTCTGATTTAATACAGATTA
ATTATGCAAAGGCAGCAGATATTGCTGTTTTGATCAAGGATAAGCAAAACTCCCTATTATCAGACAGAGGAAAAATAAGT
GTTGATGCCAGAACGAATACAATCTGGATTCAGGATACTGGCACCAAAATTGAGGAAGTAAGGGAACTGATAAAGCAATT
GGATATCCCTGTTAAGCAAGTGCTTATTGAGGCAAGAATTGTTGAAGTAACTAAAGATTTTAGCCAGGATATTGGAATAC
GCTGGGGGGTATCAAGACCAACTCACTTAAGTGGTACACTGGAAGGTGCTAATGAGTTAGCGAGAGGCACAGCACCAGCC
AATGTTGTCCCCTTGGAAAGAAGGTTAAATCTTGATTTGGCAGCTGCTCCTTTAACTGGAGCTACACCGGCCTCGGTAGG
TATTGCGCTAGCTAAACTTAATGATAACATTCTGCTTGATCTGGAGTTGTCTGCTTTGGAAAGCGAAGGGCGTGCTGAAT
TAATATCCAGTCCTCGCCTGATTACTACGAATCAACAGGCAGCAGTCATAGAGTCTGGTGAGGAGATTCCCTATCAGGAG
GCAACATCAAGTGGAGCGACTGCTGTTGCATTTAAAAAAGCAGTATTGAGTTTGAAAGTTACGCCGCAAATTACGCCTGA
TGGTAAAATTTTGATGGATTTACAGATAAATCAGGATACTCCTTCCCCACAAACATTCCTTGGTGTTCCAGCAATAATAA
CAAAGGAGATTCAGACGAATGTTCTGGTTAATAATGGACAAACCATAGTACTTGGAGGTATTTATAAGCAAGATAAGAAC
AAGGTAATCAATAGAATTCCTTTCTTTGGTGAGTTGCCTGTTGTCGGTATTTTATTTAGTAATAGACAAATCACATTGAA
AAATGAAGAATTACTTATTTTTATTACACCAAGAATAATAACGAATGCCTTATCGATTACAACGATAGAGGGACGTGAGA
AAGATGTTTATAAATAA

Domains

Predicted by InterproScan.

• AMIN domain (AMIN) (42-113)

• Bacterial type II/III secretion system short domain (Secretin_N) (370-433)

• Bacterial type II and III secretion system protein (Secretin) (525-680)

3D structure

• AlphaFold DB: Q6VY32

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilQ	Legionella pneumophila strain ERS1305867	99.713	100	0.997
pilQ	Legionella pneumophila strain Lp02	99.57	100	0.996
pilQ	Pseudomonas aeruginosa PAK	37.872	100	0.383
comQ	Acinetobacter baylyi ADP1	38.719	98.424	0.381
pilQ	Acinetobacter baumannii strain A118	35.949	100	0.361
pilQ	Acinetobacter baumannii D1279779	35.949	100	0.361