Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   CA925_RS04725 Genome accession   NZ_CP021266
Coordinates   1024225..1024821 (+) Length   198 a.a.
NCBI ID   WP_027224065.1    Uniprot ID   A0AAP3HB18
Organism   Legionella pneumophila subsp. pneumophila strain Knoxville     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1019225..1029821
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CA925_RS04710 - 1021171..1022178 (-) 1008 WP_014843598.1 hypothetical protein -
  CA925_RS04715 pilM 1022595..1023659 (+) 1065 WP_014843599.1 type IV pilus assembly protein PilM Machinery gene
  CA925_RS04720 pilN 1023663..1024211 (+) 549 WP_011213354.1 PilN domain-containing protein Machinery gene
  CA925_RS04725 pilO 1024225..1024821 (+) 597 WP_027224065.1 type 4a pilus biogenesis protein PilO Machinery gene
  CA925_RS04730 pilP 1024818..1025402 (+) 585 WP_014843601.1 pilus assembly protein PilP Machinery gene
  CA925_RS04735 pilQ 1025406..1027502 (+) 2097 WP_014843602.1 type IV pilus secretin PilQ Machinery gene
  CA925_RS04740 aroK 1027947..1028474 (+) 528 WP_010946667.1 shikimate kinase AroK -
  CA925_RS04745 aroB 1028461..1029570 (+) 1110 WP_013101269.1 3-dehydroquinate synthase -

Sequence

Protein


Download         Length: 198 a.a.        Molecular weight: 22436.16 Da        Isoelectric Point: 5.3369

>NTDB_id=229373 CA925_RS04725 WP_027224065.1 1024225..1024821(+) (pilO) [Legionella pneumophila subsp. pneumophila strain Knoxville]
MDTVNLNELTLENVGQWPMPVKIGVILGISILIIGLGYWLIVKPNFEQLEIQEAQEIALKTEFEKKQHQAANLQAYRNQL
QLMNERFGTMLKQLPAKNEMPGLLEEISKTGIASGLKFELFAPQPEVVHDFYVELPIKITIVGSYMQLARFLSRVAEMNR
IVTLHEFSVEGVSGDGKTVSQDELVMAITAKIYRYRAQ

Nucleotide


Download         Length: 597 bp        

>NTDB_id=229373 CA925_RS04725 WP_027224065.1 1024225..1024821(+) (pilO) [Legionella pneumophila subsp. pneumophila strain Knoxville]
ATGGACACGGTCAATTTAAATGAATTAACTCTAGAGAATGTTGGGCAGTGGCCAATGCCGGTTAAAATCGGAGTAATTTT
AGGCATCAGTATACTGATAATTGGATTAGGATATTGGCTTATTGTTAAGCCCAATTTTGAGCAATTGGAAATACAGGAAG
CCCAGGAAATTGCTTTGAAAACAGAGTTTGAAAAAAAGCAACATCAGGCAGCAAATCTACAAGCCTATCGTAATCAGCTA
CAGTTAATGAATGAACGCTTTGGGACTATGCTAAAACAACTTCCGGCGAAAAATGAAATGCCTGGGCTTTTGGAGGAAAT
ATCTAAAACGGGGATCGCATCGGGTTTGAAATTTGAATTGTTTGCACCACAACCTGAGGTAGTGCATGATTTTTATGTGG
AGTTACCTATAAAAATTACGATTGTCGGATCCTATATGCAATTGGCAAGATTTTTAAGTCGTGTAGCTGAAATGAACAGA
ATAGTGACTTTGCATGAATTTAGTGTTGAGGGGGTGTCCGGGGATGGTAAAACGGTCTCTCAGGATGAATTGGTGATGGC
TATCACTGCGAAAATATACAGATATCGGGCACAATGA

Domains

Predicted by InterproScan.

Pilus assembly protein, PilO (PilO)

(49-196)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Legionella pneumophila strain ERS1305867

99.497

100

1
  comO Acinetobacter baylyi ADP1

40.526

95.96

0.389

Multiple sequence alignment