Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilN   Type   Machinery gene
Locus tag   CA925_RS04720 Genome accession   NZ_CP021266
Coordinates   1023663..1024211 (+) Length   182 a.a.
NCBI ID   WP_011213354.1    Uniprot ID   A0A128KQU2
Organism   Legionella pneumophila subsp. pneumophila strain Knoxville     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1018663..1029211
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CA925_RS04710 - 1021171..1022178 (-) 1008 WP_014843598.1 hypothetical protein -
  CA925_RS04715 pilM 1022595..1023659 (+) 1065 WP_014843599.1 type IV pilus assembly protein PilM Machinery gene
  CA925_RS04720 pilN 1023663..1024211 (+) 549 WP_011213354.1 PilN domain-containing protein Machinery gene
  CA925_RS04725 pilO 1024225..1024821 (+) 597 WP_027224065.1 type 4a pilus biogenesis protein PilO Machinery gene
  CA925_RS04730 pilP 1024818..1025402 (+) 585 WP_014843601.1 pilus assembly protein PilP Machinery gene
  CA925_RS04735 pilQ 1025406..1027502 (+) 2097 WP_014843602.1 type IV pilus secretin PilQ Machinery gene
  CA925_RS04740 aroK 1027947..1028474 (+) 528 WP_010946667.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 182 a.a.        Molecular weight: 21236.95 Da        Isoelectric Point: 10.1404

>NTDB_id=229372 CA925_RS04720 WP_011213354.1 1023663..1024211(+) (pilN) [Legionella pneumophila subsp. pneumophila strain Knoxville]
MTEINLLPWREQKREQEKKLFTTMLLIGIIIAAAIVFLMNSYVSHLISNQTNRNQILQKEITALDEQIKEIKALKQIREG
LISRMSIVQNLQSTRTLMVHLFDELIKITPPGVYITKMQREHDVVTLWGYSESNTSISILMRNIEANVWIQNPVLTEIKK
NVEKKQSPNNEFKLSFVLKPKY

Nucleotide


Download         Length: 549 bp        

>NTDB_id=229372 CA925_RS04720 WP_011213354.1 1023663..1024211(+) (pilN) [Legionella pneumophila subsp. pneumophila strain Knoxville]
ATGACTGAAATTAATTTATTGCCATGGCGTGAACAAAAACGTGAGCAGGAAAAGAAATTATTTACAACCATGCTATTGAT
AGGGATTATCATCGCTGCAGCAATTGTTTTTTTAATGAACTCTTATGTCTCTCATTTAATAAGTAATCAAACGAACCGAA
ATCAGATTTTACAAAAAGAAATTACAGCTTTAGATGAACAGATCAAGGAGATTAAAGCATTAAAACAAATCAGGGAAGGA
TTAATTTCAAGAATGTCGATTGTGCAGAATCTGCAGTCAACAAGAACTTTAATGGTTCACTTATTTGATGAGTTAATAAA
AATTACTCCTCCAGGAGTTTACATAACCAAGATGCAAAGAGAACACGATGTGGTAACACTTTGGGGGTATTCCGAATCGA
ATACAAGTATTTCTATTTTGATGAGAAATATAGAGGCCAATGTCTGGATACAGAATCCTGTTCTAACTGAAATTAAAAAA
AATGTTGAAAAAAAACAGTCTCCAAACAATGAATTTAAATTAAGTTTTGTGCTTAAACCCAAATATTAG

Domains

Predicted by InterproScan.

Fimbrial assembly protein (PilN) (PilN)

(100-176)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A128KQU2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilN Legionella pneumophila strain ERS1305867

100

100

1

Multiple sequence alignment