Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   CAP31_RS01795 Genome accession   NZ_CP021138
Coordinates   343820..345541 (-) Length   573 a.a.
NCBI ID   WP_087445971.1    Uniprot ID   A0A1Y0G4R2
Organism   Sulfuriferula sp. AH1     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 338820..350541
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  CAP31_RS01765 (CAP31_01765) rpiA 339371..340033 (+) 663 WP_189836665.1 ribose-5-phosphate isomerase RpiA -
  CAP31_RS01770 (CAP31_01770) yacG 340035..340223 (-) 189 WP_087445967.1 DNA gyrase inhibitor YacG -
  CAP31_RS01775 (CAP31_01775) zapD 340220..340939 (-) 720 WP_255377122.1 cell division protein ZapD -
  CAP31_RS01780 (CAP31_01780) coaE 341046..341666 (-) 621 WP_087445969.1 dephospho-CoA kinase -
  CAP31_RS01785 (CAP31_01785) pilD 341663..342520 (-) 858 WP_087448208.1 A24 family peptidase Machinery gene
  CAP31_RS01790 (CAP31_01790) pilC 342540..343766 (-) 1227 WP_087445970.1 type II secretion system F family protein Machinery gene
  CAP31_RS01795 (CAP31_01795) pilB 343820..345541 (-) 1722 WP_087445971.1 type IV-A pilus assembly ATPase PilB Machinery gene
  CAP31_RS01800 (CAP31_01800) - 345628..346902 (-) 1275 WP_189836630.1 HlyC/CorC family transporter -
  CAP31_RS01805 (CAP31_01805) - 347001..347306 (-) 306 WP_087448210.1 hypothetical protein -
  CAP31_RS01810 (CAP31_01810) - 347563..350430 (+) 2868 WP_087445972.1 molybdopterin oxidoreductase family protein -

Sequence

Protein


Download         Length: 573 a.a.        Molecular weight: 62871.24 Da        Isoelectric Point: 5.3382

>NTDB_id=228497 CAP31_RS01795 WP_087445971.1 343820..345541(-) (pilB) [Sulfuriferula sp. AH1]
MSATSSPILLTGLARALVSQQWLTEEEAEALMKHAKSNNESFVTQVLNTKKIKSLKLAEFAATQFGFPYFDLNAIDPEVI
PKKVLDSKLIQHRRVVALYQRGNRLSVAVSDPTNLQAIDEIKFQTNLAVDIIVVEDDKLGKLVAQIGESENNTLASLDAE
DIDLDFTDDEAIAKAKANEAETADIDDAPVVRYLQKILLDAINDGVSDIHFEPYEKFYRIRYRLDGVLREIAQPPLAIKD
KLSSRIKVISKLDISEKRVPQDGRMKLVLSKNRAIDFRVSTLPTLYGEKIVMRILDPTSATLGIDALGYDPDQKALLLNA
VQRPYGMVLVTGPTGSGKTVSLYTCLNILNQPGVNIATAEDPAEINLPGVNQVNVNDKAGLTFSAALKSFLRQDPDIIMV
GEIRDLETAEIAIKAAQTGHMVLSTLHTNDAPSTLTRLMNMGIAPFNIASSVILITAQRLARRLCKCKQPADIPKAALLR
AGFGEEELDGSWLPYRAAGCDICKGTGYKGRVGIYQVMPITDVMTRIIMSNGNAIDIADQARREGVRDLRRSGLDKVRSG
LTSLEEVESVTNE

Nucleotide


Download         Length: 1722 bp        

>NTDB_id=228497 CAP31_RS01795 WP_087445971.1 343820..345541(-) (pilB) [Sulfuriferula sp. AH1]
ATGTCAGCCACTTCATCTCCGATTCTACTAACAGGTCTCGCACGCGCTCTGGTCAGTCAGCAATGGCTTACCGAGGAGGA
AGCCGAGGCCCTGATGAAGCATGCCAAGAGTAATAATGAGAGCTTTGTTACCCAGGTACTGAACACCAAAAAAATCAAGT
CGCTGAAACTGGCGGAATTTGCCGCGACCCAATTCGGCTTTCCCTACTTCGACCTGAATGCAATCGACCCCGAGGTCATC
CCTAAAAAGGTCCTCGATTCCAAGCTCATTCAGCACCGGCGTGTCGTGGCACTGTACCAGCGCGGCAACCGGTTATCGGT
AGCGGTCTCCGACCCGACCAATCTGCAAGCCATCGATGAAATCAAGTTTCAAACCAATCTGGCCGTCGATATTATCGTTG
TCGAGGATGATAAGCTCGGCAAACTGGTCGCCCAGATTGGCGAAAGCGAAAACAACACGCTGGCCAGCCTGGATGCCGAA
GATATTGATCTGGATTTTACCGATGATGAAGCCATCGCCAAAGCCAAGGCCAATGAAGCCGAGACGGCGGACATCGATGA
CGCCCCTGTCGTACGCTATCTGCAGAAGATATTGCTGGATGCGATCAATGACGGCGTGTCGGATATTCACTTCGAGCCTT
ATGAAAAATTTTACCGAATTCGCTATCGACTGGACGGCGTCCTGCGTGAAATTGCGCAACCCCCGTTAGCCATCAAAGAC
AAATTATCCTCCCGCATCAAAGTCATTTCCAAACTCGACATCTCGGAAAAACGCGTGCCGCAGGATGGCCGCATGAAACT
GGTACTGTCCAAAAACCGTGCCATCGATTTCCGTGTAAGCACCCTGCCCACCCTGTACGGCGAAAAAATCGTCATGCGGA
TTCTCGATCCCACCAGTGCCACACTGGGCATTGACGCATTGGGCTATGATCCCGATCAGAAGGCCTTGTTACTGAATGCG
GTGCAGCGTCCTTATGGCATGGTACTGGTAACCGGACCCACGGGTAGCGGCAAAACCGTGTCGCTCTACACCTGCCTGAA
CATACTTAATCAGCCGGGTGTCAATATTGCCACCGCGGAAGACCCGGCAGAAATCAACCTGCCCGGCGTCAACCAGGTCA
ACGTCAACGACAAAGCCGGACTCACGTTCTCTGCCGCACTGAAATCCTTCCTGCGCCAGGATCCGGACATTATTATGGTG
GGCGAGATACGCGATCTGGAAACCGCCGAGATTGCCATCAAAGCGGCACAAACTGGCCACATGGTATTATCGACGCTACA
TACCAACGATGCCCCATCAACGCTGACGCGTCTGATGAACATGGGGATTGCACCATTCAACATAGCATCCAGTGTTATCC
TGATTACCGCCCAGCGACTGGCGCGCAGATTATGCAAATGCAAACAGCCAGCCGACATCCCCAAAGCCGCGCTGCTGCGT
GCAGGATTTGGTGAAGAGGAACTGGACGGCAGCTGGCTGCCATACCGTGCCGCAGGCTGTGATATTTGCAAAGGGACGGG
ATACAAGGGGCGAGTTGGCATTTATCAGGTCATGCCGATCACAGACGTAATGACGCGCATCATCATGAGCAACGGCAACG
CCATCGACATTGCGGATCAGGCGCGACGCGAAGGCGTCAGGGATCTGAGGCGCTCGGGACTGGATAAAGTCCGTTCAGGA
CTAACCTCGCTGGAAGAAGTCGAATCGGTGACTAACGAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(196-463)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(17-150)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1Y0G4R2

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

56.522

100

0.567
  pilB Acinetobacter baylyi ADP1

55.304

100

0.555
  pilF Neisseria gonorrhoeae MS11

54.657

99.302

0.543
  pilB Legionella pneumophila strain ERS1305867

51.916

100

0.52
  pilB Vibrio cholerae strain A1552

47.535

99.127

0.471
  pilB Vibrio parahaemolyticus RIMD 2210633

46.046

99.302

0.457
  pilB Vibrio campbellii strain DS40M4

45.87

99.302

0.455
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

40.816

94.066

0.384
  pilB/pilB1 Synechocystis sp. PCC 6803

39.781

95.637

0.38
  pilF Thermus thermophilus HB27

37.478

100

0.379

Multiple sequence alignment