Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   B6D87_RS03940 Genome accession   NZ_CP021132
Coordinates   824741..825958 (-) Length   405 a.a.
NCBI ID   WP_086795852.1    Uniprot ID   -
Organism   Pseudomonas fragi strain NMC25     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 819741..830958
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  B6D87_RS03905 (B6D87_03900) - 820773..820946 (-) 174 WP_016779441.1 DUF3094 family protein -
  B6D87_RS03910 (B6D87_03905) - 821001..821528 (-) 528 WP_086795850.1 MOSC domain-containing protein -
  B6D87_RS03915 (B6D87_03910) - 821525..822154 (-) 630 WP_016779443.1 DUF1780 domain-containing protein -
  B6D87_RS03920 (B6D87_03915) - 822264..822950 (+) 687 WP_083369773.1 energy-coupling factor ABC transporter permease -
  B6D87_RS03925 (B6D87_03920) yacG 823053..823256 (-) 204 WP_016779444.1 DNA gyrase inhibitor YacG -
  B6D87_RS03930 (B6D87_03925) coaE 823253..823876 (-) 624 WP_016779445.1 dephospho-CoA kinase -
  B6D87_RS03935 (B6D87_03930) - 823878..824744 (-) 867 WP_167380468.1 A24 family peptidase -
  B6D87_RS03940 (B6D87_03935) pilC 824741..825958 (-) 1218 WP_086795852.1 type II secretion system F family protein Machinery gene
  B6D87_RS03945 (B6D87_03940) - 826170..826577 (+) 408 WP_016779448.1 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  B6D87_RS03955 (B6D87_03950) - 826869..828359 (+) 1491 WP_086795853.1 DNA-3-methyladenine glycosylase 2 -
  B6D87_RS03960 (B6D87_03955) - 828362..828853 (+) 492 WP_016779450.1 methylated-DNA--[protein]-cysteine S-methyltransferase -
  B6D87_RS03965 (B6D87_03960) nadC 829832..830680 (-) 849 WP_016779451.1 carboxylating nicotinate-nucleotide diphosphorylase -

Sequence

Protein


Download         Length: 405 a.a.        Molecular weight: 43854.48 Da        Isoelectric Point: 9.8101

>NTDB_id=228464 B6D87_RS03940 WP_086795852.1 824741..825958(-) (pilC) [Pseudomonas fragi strain NMC25]
MATQAVKNTLYRWQGTDGNGVKTSGQISADSHAMIRVQLRRQGITVSKIRRHYSRDRDAGKSITALDITLFTRQLATLIS
AGIALQQALGVIADGFESRAMRQQVTELKQDIGAGTSLAAALRKKPAHFDPLFCSLVEAGELAGALDVMLQRVATYREKT
EALKKKIKKTMTYPSAVLLVALGVCSILLIAVVPQFQGVFRSFDAQLPALTLWVIGLSDGLQAHGLWLLCVCGFILAGLH
HRYKHCPALRQRIDRWLLHLPVLGNLLHMSAIARFARTLSTTLAAGVPLLEALNSVASATRNNLHKDAVLRLKQQVATGS
PIHVAMAAVGVFPGMAIQMTAIGEESGTLDSMLDHVAAYYESEVDAQVDHLTTLLEPIIMVVLGVMVGGLVIAMYLPIFN
LGQVI

Nucleotide


Download         Length: 1218 bp        

>NTDB_id=228464 B6D87_RS03940 WP_086795852.1 824741..825958(-) (pilC) [Pseudomonas fragi strain NMC25]
ATGGCAACACAGGCCGTTAAAAACACCCTCTACCGGTGGCAAGGCACAGATGGCAACGGCGTTAAAACCAGTGGCCAGAT
CTCGGCTGACAGCCACGCCATGATCAGGGTCCAACTACGCCGCCAAGGTATTACCGTTAGTAAAATCCGCCGCCATTACA
GTCGCGATCGCGATGCAGGCAAATCCATCACGGCGCTGGATATCACCCTGTTCACCCGCCAGTTGGCAACCCTGATCAGC
GCCGGCATTGCCTTGCAGCAAGCCCTGGGGGTGATCGCCGACGGTTTCGAGAGCCGGGCCATGCGCCAGCAGGTCACCGA
ACTCAAACAGGACATCGGCGCAGGCACCAGCCTGGCGGCAGCACTGCGCAAAAAACCGGCACATTTCGACCCGCTGTTTT
GCAGCCTCGTAGAAGCGGGGGAACTGGCCGGGGCGCTGGACGTCATGCTGCAACGGGTAGCGACCTACCGGGAGAAAACC
GAAGCCCTGAAAAAGAAAATCAAAAAAACCATGACCTACCCCAGCGCCGTGCTCTTGGTGGCGCTGGGGGTGTGCAGTAT
TTTGCTGATCGCAGTGGTACCGCAGTTTCAGGGCGTGTTCCGCTCATTCGATGCGCAACTGCCGGCGCTGACCTTGTGGG
TCATCGGCCTGTCTGATGGCCTGCAGGCACATGGCCTGTGGCTGCTGTGTGTGTGCGGCTTCATTCTGGCCGGCCTTCAT
CATCGCTACAAACATTGTCCAGCCTTGCGCCAGCGCATTGATCGCTGGCTGCTGCATTTGCCCGTGCTGGGCAATCTGCT
GCACATGTCAGCCATCGCCCGTTTTGCGCGCACGCTGTCGACCACCCTGGCTGCCGGGGTGCCATTGCTCGAAGCGCTGA
ATAGTGTCGCCAGCGCCACGCGCAATAACCTGCACAAGGACGCGGTGCTGCGGCTCAAACAACAGGTTGCGACTGGTAGC
CCGATCCATGTGGCAATGGCTGCAGTCGGAGTATTCCCGGGCATGGCAATTCAGATGACAGCAATTGGCGAAGAATCCGG
CACATTAGACAGCATGCTGGACCACGTTGCCGCCTATTATGAAAGCGAGGTCGACGCACAGGTCGACCACCTCACAACGT
TGCTGGAGCCGATCATCATGGTGGTACTTGGAGTTATGGTTGGCGGGCTGGTCATCGCCATGTACTTGCCGATCTTCAAC
CTGGGCCAGGTAATCTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(71-194)

Type II secretion system (T2SS), protein F (T2SSF)

(275-397)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

53.071

100

0.533
  pilC Acinetobacter baumannii D1279779

49.622

98.025

0.486
  pilC Acinetobacter baylyi ADP1

48.995

98.272

0.481
  pilC Legionella pneumophila strain ERS1305867

49.118

98.025

0.481
  pilC Vibrio cholerae strain A1552

41.975

100

0.42
  pilC Vibrio campbellii strain DS40M4

39.803

100

0.4
  pilG Neisseria gonorrhoeae MS11

39.267

94.321

0.37
  pilG Neisseria meningitidis 44/76-A

39.005

94.321

0.368
  pilC Thermus thermophilus HB27

37

98.765

0.365

Multiple sequence alignment