Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   B6N58_RS09595 Genome accession   NZ_CP020614
Coordinates   2145817..2147316 (-) Length   499 a.a.
NCBI ID   WP_253675030.1    Uniprot ID   -
Organism   Legionella micdadei strain NZ2015     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2140817..2152316
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  B6N58_RS09570 (B6N58_09570) - 2140847..2141284 (-) 438 WP_045098903.1 universal stress protein -
  B6N58_RS09575 (B6N58_09575) pnuC 2141659..2142225 (+) 567 WP_045098902.1 nicotinamide riboside transporter PnuC -
  B6N58_RS09580 (B6N58_09580) - 2142430..2143893 (-) 1464 WP_045098901.1 AAA family ATPase -
  B6N58_RS09585 (B6N58_09585) aroB 2143895..2145001 (-) 1107 WP_045098900.1 3-dehydroquinate synthase -
  B6N58_RS09590 (B6N58_09590) aroK 2144988..2145515 (-) 528 WP_045098899.1 shikimate kinase AroK -
  B6N58_RS09595 (B6N58_09595) pilQ 2145817..2147316 (-) 1500 WP_253675030.1 type IV pilus secretin PilQ Machinery gene
  B6N58_RS09600 (B6N58_09600) - 2147349..2147861 (-) 513 WP_045098898.1 pilus assembly protein PilP -
  B6N58_RS09605 (B6N58_09605) pilO 2147862..2148338 (-) 477 WP_161939853.1 type 4a pilus biogenesis protein PilO -
  B6N58_RS09610 (B6N58_09610) - 2148467..2149012 (-) 546 WP_045098896.1 PilN domain-containing protein -
  B6N58_RS09615 (B6N58_09615) pilM 2149002..2149973 (-) 972 WP_161939854.1 type IV pilus assembly protein PilM -
  B6N58_RS09620 (B6N58_09620) - 2150315..2151286 (+) 972 WP_045098894.1 hypothetical protein -

Sequence

Protein


Download         Length: 499 a.a.        Molecular weight: 54942.97 Da        Isoelectric Point: 9.9615

>NTDB_id=224967 B6N58_RS09595 WP_253675030.1 2145817..2147316(-) (pilQ) [Legionella micdadei strain NZ2015]
MSRIILILILIIGNDCLFAMDSNVNGTKLSRVNQENKQEIVQTLPMEQKSYNNKDMVKFISLNFQEIKVSAVLQLLADFT
GINIVVSDKVTSSLSLRLDDILWDQALDIILTTRSLSKRKIGNVMFVAPVDEIIELEKKEFSMENDRRNFAPLRSDLLQV
NYAKAADIANLLTDPKTTLLSPRGSVNVDARTNTIWLQDIGTHVKKIRELVKRLDVPVKQVLIEARIVNATKDFARDLGV
RFGVSRPNHLVGRLKGIKQSVPYSGMEEEVPLAERLNLDLAALPITASPASVGVALAKLGNGILLDLELSALESEGKGEI
IANPRLITTNQQTAVIESGEEIPYQEATLSGSTAVAFKKAVLSLKVTPQITPDSKILMDLQINQNIPSVKVFNGVPSILT
KEIQTSVLINDGQTVVLGGIYKRDKYNALNRVPFLGELPVVGRLFRNKTTAVKNEELLIFITPRIITNNLSAVTMEGRRQ
AIAGGVKLGKFGKRVGSIH

Nucleotide


Download         Length: 1500 bp        

>NTDB_id=224967 B6N58_RS09595 WP_253675030.1 2145817..2147316(-) (pilQ) [Legionella micdadei strain NZ2015]
TTGAGTAGGATTATTCTAATTCTTATATTGATAATAGGAAATGATTGTTTGTTTGCAATGGATAGTAACGTAAATGGGAC
TAAATTGAGTCGCGTTAATCAGGAGAATAAACAGGAAATAGTCCAGACGCTACCTATGGAGCAGAAATCCTATAATAATA
AGGATATGGTCAAGTTTATTTCTTTAAATTTTCAGGAGATTAAAGTAAGCGCTGTCCTGCAATTATTGGCTGATTTTACT
GGAATAAATATAGTAGTCAGTGATAAAGTAACAAGTAGCTTAAGTTTGCGTTTGGATGACATACTTTGGGACCAAGCATT
GGATATTATTCTTACAACTCGATCTTTGAGTAAGCGAAAAATAGGCAATGTAATGTTTGTAGCTCCTGTTGATGAAATCA
TAGAGCTTGAAAAAAAAGAATTTAGTATGGAGAATGATCGCAGAAATTTTGCACCCCTGCGATCAGACTTACTACAAGTT
AATTATGCAAAAGCAGCTGATATTGCTAATTTACTAACAGACCCAAAAACTACACTATTATCTCCTCGAGGTAGTGTAAA
TGTGGATGCAAGAACCAACACCATTTGGTTACAGGATATTGGGACTCATGTTAAAAAAATAAGGGAGCTTGTAAAGCGCC
TTGATGTCCCTGTTAAACAAGTACTCATCGAGGCACGCATTGTCAATGCGACAAAAGATTTTGCAAGGGATTTGGGTGTC
CGTTTTGGTGTATCTAGGCCAAACCATTTAGTTGGCCGGTTAAAAGGCATTAAGCAGTCAGTACCATATAGTGGAATGGA
AGAAGAGGTCCCTTTGGCTGAACGTCTAAATCTTGATCTGGCTGCGCTACCAATTACAGCGTCGCCGGCATCAGTTGGCG
TTGCTTTAGCGAAATTGGGTAATGGTATACTTCTCGATTTAGAGCTTTCTGCACTTGAAAGTGAAGGAAAGGGAGAAATA
ATTGCTAATCCTAGATTAATAACAACCAATCAACAGACAGCTGTGATTGAGTCGGGAGAGGAAATACCATACCAAGAAGC
GACTTTGAGTGGGTCAACTGCTGTCGCATTCAAGAAAGCAGTACTAAGCTTAAAGGTGACACCACAAATTACACCAGATA
GTAAGATTTTGATGGATTTGCAGATTAATCAAAATATACCGTCTGTGAAGGTATTTAATGGAGTTCCTTCTATTTTAACA
AAGGAAATTCAGACAAGTGTCCTGATTAATGATGGACAAACGGTTGTTTTAGGGGGTATTTATAAAAGAGATAAGTACAA
TGCACTAAATCGTGTTCCTTTCCTAGGAGAATTGCCTGTCGTGGGACGATTATTTCGAAACAAAACGACTGCGGTTAAAA
ATGAGGAGTTGCTCATTTTCATTACTCCTAGGATAATAACAAACAACTTGTCAGCAGTAACGATGGAAGGGCGAAGGCAA
GCGATTGCAGGAGGTGTTAAGTTGGGCAAGTTTGGTAAGCGAGTAGGATCGATTCATTAG

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(158-220)

Bacterial type II and III secretion system protein (Secretin)

(311-466)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Legionella pneumophila strain Lp02

63.755

91.784

0.585
  pilQ Legionella pneumophila strain ERS1305867

63.755

91.784

0.585
  comQ Acinetobacter baylyi ADP1

42.072

94.79

0.399
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

47.002

83.567

0.393
  pilQ Vibrio cholerae strain A1552

47.002

83.567

0.393
  pilQ Pseudomonas aeruginosa PAK

41.542

93.587

0.389
  pilQ Vibrio campbellii strain DS40M4

45.647

85.17

0.389
  pilQ Acinetobacter baumannii D1279779

40.305

91.984

0.371
  pilQ Acinetobacter baumannii strain A118

40.305

91.984

0.371

Multiple sequence alignment