Detailed information NTG0222894

Bioinformatically predicted

Overview

• Name: comYF
• Type: Machinery gene
• Locus tag: A6J31_RS08660
• Genome accession: NZ_CP020431
• Coordinates: 1685498..1685935 (-)
• Length: 145 a.a.
• NCBI ID: WP_002887014.1
• Uniprot ID: A0A1V0GF29
• Organism: Streptococcus sp. FDAARGOS_192
• Function: dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 1680498..1690935

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
A6J31_RS08625 (A6J31_08560)	-	1680560..1681222 (-)	663	WP_080610969.1	CPBP family intramembrane glutamic endopeptidase	-
A6J31_RS08630 (A6J31_08565)	-	1681335..1681781 (-)	447	WP_080610970.1	CAAX protease	-
A6J31_RS08635 (A6J31_08570)	-	1681859..1682515 (-)	657	WP_014632546.1	CPBP family intramembrane glutamic endopeptidase	-
A6J31_RS08640 (A6J31_08575)	-	1682527..1682724 (-)	198	WP_002885716.1	helix-turn-helix transcriptional regulator	-
A6J31_RS08645 (A6J31_08580)	-	1682952..1684145 (-)	1194	WP_002885831.1	acetate kinase	-
A6J31_RS08650 (A6J31_08585)	comYH	1684202..1685158 (-)	957	WP_080610972.1	class I SAM-dependent methyltransferase	Machinery gene
A6J31_RS08655 (A6J31_08590)	comGG	1685203..1685520 (-)	318	WP_021144683.1	competence type IV pilus minor pilin ComGG	-
A6J31_RS08660 (A6J31_08595)	comYF	1685498..1685935 (-)	438	WP_002887014.1	competence type IV pilus minor pilin ComGF	Machinery gene
A6J31_RS08665 (A6J31_08600)	comGE	1685922..1686152 (-)	231	WP_002887015.1	competence type IV pilus minor pilin ComGE	-
A6J31_RS08670 (A6J31_08605)	comYD	1686184..1686612 (-)	429	WP_263970040.1	competence type IV pilus minor pilin ComGD	Machinery gene
A6J31_RS08675 (A6J31_08610)	comYC	1686572..1686886 (-)	315	WP_037611125.1	competence type IV pilus major pilin ComGC	Machinery gene
A6J31_RS08680 (A6J31_08615)	comYB	1686895..1687995 (-)	1101	WP_142390728.1	competence type IV pilus assembly protein ComGB	Machinery gene
A6J31_RS08685 (A6J31_08620)	comYA	1687877..1688818 (-)	942	WP_080610975.1	competence type IV pilus ATPase ComGA	Machinery gene
A6J31_RS08690 (A6J31_08625)	-	1688898..1689260 (-)	363	WP_002887019.1	DUF1033 family protein	-

Sequence

Protein

• Length: 145 a.a.
• Molecular weight: 16155.57 Da
• Isoelectric Point: 8.4658

>NTDB_id=222894 A6J31_RS08660 WP_002887014.1 1685498..1685935(-) (comYF) [Streptococcus sp. FDAARGOS_192]
MSLNSKVRAFTLLECLVALLVIAGSVQVYQGLTTVLVSNVKQVKQQESQDWLLFVQQMEAELEGCQLVKVEGNKLYVKQD
NQDLAFGLSSATDFRKTNADGRGYQPMLFNVKASTISQNNQIVTIQVTLKNGLQRSFVYAFEKTG

Nucleotide

• Length: 438 bp

>NTDB_id=222894 A6J31_RS08660 WP_002887014.1 1685498..1685935(-) (comYF) [Streptococcus sp. FDAARGOS_192]
ATGTCTCTCAATAGCAAGGTTCGTGCTTTTACCCTCTTGGAATGTCTAGTGGCCTTGTTGGTGATTGCTGGTTCAGTTCA
AGTTTATCAAGGTCTGACCACAGTATTGGTGAGCAATGTTAAGCAGGTCAAGCAACAAGAAAGCCAAGACTGGCTTCTAT
TTGTTCAACAAATGGAGGCAGAATTAGAAGGTTGTCAGTTGGTAAAGGTAGAGGGGAACAAACTCTATGTCAAGCAGGAT
AATCAAGACTTAGCTTTTGGACTATCAAGTGCTACTGATTTTCGAAAAACAAATGCTGACGGTCGAGGCTACCAACCAAT
GCTTTTCAATGTAAAAGCTAGTACAATTAGTCAAAATAACCAGATAGTCACCATCCAAGTGACTCTTAAAAATGGGCTAC
AAAGGAGTTTTGTTTATGCTTTTGAAAAAACAGGTTAG

Domains

Predicted by InterproScan.

• Putative Competence protein ComGF (ComGF) (39-134)

3D structure

• AlphaFold DB: A0A1V0GF29

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
comYF	Streptococcus mutans UA140	58.156	97.241	0.566
comYF	Streptococcus mutans UA159	57.447	97.241	0.559
comGF/cglF	Streptococcus pneumoniae Rx1	50	97.931	0.49
comGF/cglF	Streptococcus pneumoniae D39	50	97.931	0.49
comGF/cglF	Streptococcus pneumoniae R6	50	97.931	0.49
comGF/cglF	Streptococcus pneumoniae TIGR4	50	97.931	0.49
comGF/cglF	Streptococcus mitis SK321	47.222	99.31	0.469
comGF/cglF	Streptococcus mitis NCTC 12261	47.222	99.31	0.469
comGF	Lactococcus lactis subsp. cremoris KW2	45.324	95.862	0.434