Detailed information    

insolico Bioinformatically predicted

Overview

Name   comYA   Type   Machinery gene
Locus tag   SAG_RS03550 Genome accession   NC_004116
Coordinates   187282..188253 (+) Length   323 a.a.
NCBI ID   WP_000250805.1    Uniprot ID   -
Organism   Streptococcus agalactiae 2603V/R     
Function   dsDNA binding to the cell surface; assembly of the pseudopilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 182282..193253
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  SAG_RS03540 (SAG0161) rpoC 182980..186630 (+) 3651 WP_000228729.1 DNA-directed RNA polymerase subunit beta' -
  SAG_RS03545 (SAG0162) - 186744..187109 (+) 366 WP_000285373.1 DUF1033 family protein -
  SAG_RS03550 (SAG0163) comYA 187282..188253 (+) 972 WP_000250805.1 competence type IV pilus ATPase ComGA Machinery gene
  SAG_RS03555 (SAG0164) comYB 188147..189190 (+) 1044 WP_223295889.1 competence type IV pilus assembly protein ComGB Machinery gene
  SAG_RS03560 (SAG0165) comGF 189187..189642 (+) 456 WP_000793378.1 competence type IV pilus minor pilin ComGF -
  SAG_RS03565 (SAG0166) comGG 189620..189991 (+) 372 WP_000601104.1 competence type IV pilus minor pilin ComGG -
  SAG_RS03570 (SAG0167) comYH 190106..191080 (+) 975 WP_001008574.1 class I SAM-dependent methyltransferase Machinery gene
  SAG_RS03575 (SAG0168) - 191112..192305 (+) 1194 WP_000047534.1 acetate kinase -
  SAG_RS03580 (SAG0169) - 192457..192663 (+) 207 WP_000798241.1 helix-turn-helix transcriptional regulator -
  SAG_RS13410 (SAG0170) - 192722..192859 (+) 138 WP_001865900.1 hypothetical protein -

Sequence

Protein


Download         Length: 323 a.a.        Molecular weight: 36801.32 Da        Isoelectric Point: 6.8469

>NTDB_id=22252 SAG_RS03550 WP_000250805.1 187282..188253(+) (comYA) [Streptococcus agalactiae 2603V/R]
MVQSLAKQVIHQAVEVNAQDIYIIPKGDCYELYMRIDDERRFIDVFEFNRMASLISHFKFVAGMNVGEKRRSQLGSCDYE
LSEGRLVSLRLSSVGDYRGQESLVIRILYSGHQDLKYWFDNIKQMKEVLGIRGLYLFSGPVGSGKTTLMYQLASEVFKNK
QIITIEDPVEIKNDKMLQLQLNEDIGMTYDALIKLSLRHRPDILIIGEIRDQATARAVIRASLTGVMVFSTIHAKSIPGV
YDRLIELGVNYQELENSLKLIAYQRLIGGGSLIDFETGNFKKHSSDKWNRQVDILAEEGHISKKQAQVEKIIPQETTESS
PTF

Nucleotide


Download         Length: 972 bp        

>NTDB_id=22252 SAG_RS03550 WP_000250805.1 187282..188253(+) (comYA) [Streptococcus agalactiae 2603V/R]
ATGGTTCAATCATTAGCAAAGCAAGTCATTCATCAGGCAGTAGAAGTAAATGCTCAAGATATTTATATCATTCCCAAAGG
TGATTGTTATGAACTCTATATGCGTATTGATGATGAAAGGCGGTTTATTGATGTTTTTGAGTTTAATAGGATGGCTAGTC
TTATTAGTCACTTTAAATTTGTGGCAGGCATGAACGTTGGAGAAAAAAGACGAAGTCAATTAGGTTCTTGTGACTATGAA
CTGTCAGAGGGAAGACTGGTTTCATTACGACTATCGAGTGTGGGAGATTATCGTGGTCAAGAATCTTTAGTTATTCGTAT
TTTGTATTCAGGTCATCAGGACTTAAAATATTGGTTTGATAATATAAAGCAAATGAAGGAAGTACTGGGTATAAGAGGGC
TATATCTTTTTTCCGGCCCTGTGGGGAGTGGTAAAACAACTCTCATGTATCAATTAGCTTCAGAAGTATTTAAAAATAAG
CAAATTATCACGATTGAAGATCCGGTAGAAATCAAGAATGACAAGATGTTACAACTCCAATTGAATGAGGATATTGGAAT
GACTTATGATGCTTTAATCAAACTGTCTTTACGGCATCGTCCAGATATTTTAATTATCGGAGAGATTAGAGATCAAGCGA
CGGCCCGTGCTGTTATTCGTGCAAGTTTAACGGGAGTGATGGTTTTTTCTACTATTCATGCTAAAAGTATTCCCGGAGTC
TATGATAGGCTTATAGAATTAGGGGTTAACTATCAAGAGTTAGAAAATAGTCTAAAATTAATAGCATATCAACGTTTAAT
TGGAGGAGGAAGCCTAATTGACTTTGAGACAGGTAATTTTAAAAAACACTCATCAGACAAGTGGAATAGACAAGTGGATA
TCTTGGCTGAAGAAGGACATATCAGTAAGAAACAGGCACAAGTCGAAAAAATTATCCCTCAAGAAACAACGGAAAGTAGT
CCAACTTTTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(8-267)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comYA Streptococcus mutans UA159

68.81

96.285

0.663
  comYA Streptococcus mutans UA140

68.81

96.285

0.663
  comYA Streptococcus gordonii str. Challis substr. CH1

63.636

98.762

0.628
  comGA/cglA/cilD Streptococcus mitis NCTC 12261

62.701

96.285

0.604
  comGA/cglA/cilD Streptococcus pneumoniae Rx1

61.736

96.285

0.594
  comGA/cglA/cilD Streptococcus pneumoniae D39

61.736

96.285

0.594
  comGA/cglA/cilD Streptococcus pneumoniae R6

61.736

96.285

0.594
  comGA/cglA/cilD Streptococcus pneumoniae TIGR4

61.736

96.285

0.594
  comGA/cglA Streptococcus sobrinus strain NIDR 6715-7

61.935

95.975

0.594
  comGA Lactococcus lactis subsp. cremoris KW2

50.621

99.69

0.505

Multiple sequence alignment