Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   THSYN_RS28130 Genome accession   NZ_CP020370
Coordinates   6570818..6572077 (+) Length   419 a.a.
NCBI ID   WP_100922042.1    Uniprot ID   A0A2K8UFP5
Organism   Candidatus Thiodictyon syntrophicum strain Cad16T     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 6565818..6577077
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  THSYN_RS28115 (THSYN_27905) acs 6565843..6567789 (+) 1947 WP_100922039.1 acetate--CoA ligase -
  THSYN_RS28120 (THSYN_27910) - 6568080..6568913 (+) 834 WP_100922040.1 NYN domain-containing protein -
  THSYN_RS28125 (THSYN_27915) pilB 6569100..6570812 (+) 1713 WP_100922041.1 type IV-A pilus assembly ATPase PilB Machinery gene
  THSYN_RS28130 (THSYN_27920) pilC 6570818..6572077 (+) 1260 WP_100922042.1 type II secretion system F family protein Machinery gene
  THSYN_RS28135 (THSYN_27925) pilD 6572329..6573201 (+) 873 WP_100922043.1 prepilin peptidase Machinery gene
  THSYN_RS28140 (THSYN_27930) coaE 6573217..6573813 (+) 597 WP_100922044.1 dephospho-CoA kinase -
  THSYN_RS28145 (THSYN_27935) ilvB 6574099..6575844 (+) 1746 WP_100922045.1 biosynthetic-type acetolactate synthase large subunit -
  THSYN_RS28150 (THSYN_27940) - 6576168..6576863 (-) 696 WP_100922046.1 anaerobic ribonucleoside-triphosphate reductase activating protein -

Sequence

Protein


Download         Length: 419 a.a.        Molecular weight: 45673.86 Da        Isoelectric Point: 10.0580

>NTDB_id=221840 THSYN_RS28130 WP_100922042.1 6570818..6572077(+) (pilC) [Candidatus Thiodictyon syntrophicum strain Cad16T]
MALAAAQRPRPKPKAPPKAEKAYVFVWEGTDRRGGRVKGETRASSITTVRADLRRQGVNPTKVRKKAQSLFSGRKKIGAG
DIAIFSRQLATMMAAGVPLVQSFDIVGRGHDNAAMQDLILAIKQDIESGTAMAEALGKHPLYFDDLFCNLVAAGEQAGVL
DILLDKIAVYKEKTESIKGKIKKALFYPTAVIAVAILVTVVILIFVIPQFKELFSSFGADLPAFTLAVIAMSDVLREWWW
AVFLVVAGAIWAIGYVFKRSRKFREIVDRTLLKIPIIGVILNKASLARFARTLSTMFAAGVPLVEALESVSGATGNIVYQ
EAVLKMREEVATGQSLQLSMRQRNLFPHMVIQMTAIGEESGALDEMLAKVADFYEEQVDNAVDSLSSLLEPMIMVVIGGL
VGSLVVAMYLPIFKLASVV

Nucleotide


Download         Length: 1260 bp        

>NTDB_id=221840 THSYN_RS28130 WP_100922042.1 6570818..6572077(+) (pilC) [Candidatus Thiodictyon syntrophicum strain Cad16T]
ATGGCACTGGCAGCAGCGCAGAGGCCCAGGCCTAAACCCAAGGCCCCGCCCAAGGCTGAAAAGGCCTATGTTTTCGTTTG
GGAAGGAACCGACCGCCGCGGCGGCCGTGTCAAAGGCGAAACCCGCGCCTCGAGCATCACGACGGTGCGCGCCGACCTGC
GCCGCCAGGGGGTAAACCCCACAAAAGTCAGGAAAAAGGCCCAGTCGCTCTTCAGCGGCAGAAAGAAGATCGGCGCCGGT
GACATCGCCATCTTCAGCCGCCAACTGGCGACCATGATGGCCGCCGGTGTGCCCTTGGTGCAGTCCTTCGACATCGTCGG
CCGCGGACACGACAACGCGGCCATGCAGGACTTGATCCTCGCCATCAAGCAGGATATCGAGAGCGGCACCGCCATGGCGG
AGGCCCTGGGCAAGCACCCACTCTATTTCGATGACCTGTTCTGCAATCTGGTCGCCGCCGGTGAGCAGGCCGGTGTGCTG
GACATCCTGTTGGACAAGATTGCGGTCTACAAGGAAAAAACCGAATCCATCAAGGGCAAGATCAAGAAAGCCCTGTTCTA
TCCCACGGCGGTCATTGCCGTCGCCATCCTCGTCACGGTGGTGATCCTGATCTTCGTCATCCCTCAGTTCAAGGAACTGT
TCAGCAGCTTCGGGGCGGACCTGCCGGCCTTCACCCTGGCGGTCATCGCCATGTCAGACGTGCTGCGGGAGTGGTGGTGG
GCGGTTTTTCTGGTCGTTGCCGGGGCAATCTGGGCGATCGGCTATGTCTTCAAGCGCTCCCGCAAGTTTCGCGAGATCGT
CGACCGCACGCTCCTGAAGATCCCGATCATCGGCGTTATCCTCAATAAGGCATCATTGGCGCGCTTTGCCCGGACCCTAT
CCACCATGTTCGCCGCCGGTGTCCCCTTGGTGGAGGCACTGGAATCGGTGTCCGGCGCCACCGGCAACATCGTCTATCAG
GAGGCGGTGCTCAAGATGCGCGAGGAGGTGGCCACCGGCCAGTCCCTGCAACTGTCCATGCGGCAGCGCAACCTGTTTCC
CCACATGGTGATCCAGATGACTGCCATCGGCGAGGAGTCCGGCGCCCTGGACGAGATGCTCGCCAAGGTCGCCGACTTCT
ACGAGGAGCAGGTGGACAATGCCGTCGACAGTCTCAGCAGCCTCTTGGAGCCGATGATCATGGTGGTCATCGGCGGCCTG
GTGGGCAGCCTGGTGGTGGCCATGTACCTGCCGATCTTCAAGCTTGCCTCCGTGGTGTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(85-208)

Type II secretion system (T2SS), protein F (T2SSF)

(289-411)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2K8UFP5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

58.458

95.943

0.561
  pilC Legionella pneumophila strain ERS1305867

59.091

94.511

0.558
  pilC Acinetobacter baylyi ADP1

54.054

97.136

0.525
  pilC Acinetobacter baumannii D1279779

53.317

97.136

0.518
  pilG Neisseria gonorrhoeae MS11

46.02

95.943

0.442
  pilG Neisseria meningitidis 44/76-A

46.02

95.943

0.442
  pilC Vibrio cholerae strain A1552

41.162

94.511

0.389
  pilC Vibrio campbellii strain DS40M4

40.399

95.704

0.387

Multiple sequence alignment