Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   THSYN_RS28125 Genome accession   NZ_CP020370
Coordinates   6569100..6570812 (+) Length   570 a.a.
NCBI ID   WP_100922041.1    Uniprot ID   A0A2K8UFQ6
Organism   Candidatus Thiodictyon syntrophicum strain Cad16T     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 6564100..6575812
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  THSYN_RS28110 (THSYN_27900) - 6564331..6564870 (+) 540 WP_100922038.1 DUF86 domain-containing protein -
  THSYN_RS34445 - 6564907..6565374 (+) 468 WP_157817990.1 hypothetical protein -
  THSYN_RS28115 (THSYN_27905) acs 6565843..6567789 (+) 1947 WP_100922039.1 acetate--CoA ligase -
  THSYN_RS28120 (THSYN_27910) - 6568080..6568913 (+) 834 WP_100922040.1 NYN domain-containing protein -
  THSYN_RS28125 (THSYN_27915) pilB 6569100..6570812 (+) 1713 WP_100922041.1 type IV-A pilus assembly ATPase PilB Machinery gene
  THSYN_RS28130 (THSYN_27920) pilC 6570818..6572077 (+) 1260 WP_100922042.1 type II secretion system F family protein Machinery gene
  THSYN_RS28135 (THSYN_27925) pilD 6572329..6573201 (+) 873 WP_100922043.1 prepilin peptidase Machinery gene
  THSYN_RS28140 (THSYN_27930) coaE 6573217..6573813 (+) 597 WP_100922044.1 dephospho-CoA kinase -

Sequence

Protein


Download         Length: 570 a.a.        Molecular weight: 63149.51 Da        Isoelectric Point: 5.4795

>NTDB_id=221839 THSYN_RS28125 WP_100922041.1 6569100..6570812(+) (pilB) [Candidatus Thiodictyon syntrophicum strain Cad16T]
MATNQTTMKPSGLAWRLVRDRLLTEDQATTATTEALRRREPFVKYLVDQKVLDSRRIAIAASHEFGVPLLDLSALELVDM
PLNLVDEKLIRKHRALPLFRRGNRIFLALSDPTNGQALEEIRFNTGLATDAVLVEEDKLAAAIDRAIDAQDTTMTDLMDT
DLDNLDIAADDEDQHDDSDLNVDDAPVVRYVNKILVDAITNSASDIHFEPYEKYFRIRFRQDGLLREVANPPLNIAARLV
ARLKVMSRMNIAERRVPQDGRIKLKLSRTRDIDFRVNTLPCLHGEKVVLRILDSTSAQVGIDALGFEPEQRDLFLAAIKK
PYGMILVTGPTGSGKTVSLYSAINILNAPDVNISTVEDPVEIQVAGINQVNMNPKTGLTFASALRAFLRQDPDILMVGEI
RDLETAEIAVKAAQTGHMVLSTLHTNDAPQTLTRLANMGVAPFNIASSILLIMAQRLTRKLCPSCKQPEEVPREALREEG
FTEADLDEGITVYRPVGCERCTKGYKGRIGIFQVMKVSEAIGRLIMEGGNALQLAEQAAREGVANLRQSGLRKVKAGITS
LEEINRVTKD

Nucleotide


Download         Length: 1713 bp        

>NTDB_id=221839 THSYN_RS28125 WP_100922041.1 6569100..6570812(+) (pilB) [Candidatus Thiodictyon syntrophicum strain Cad16T]
ATGGCGACCAATCAGACCACCATGAAACCGTCGGGCCTGGCGTGGCGCCTGGTGCGGGATCGCTTGCTGACCGAGGACCA
GGCGACCACCGCGACCACCGAGGCACTGCGGCGCCGCGAGCCCTTCGTCAAGTACTTAGTCGATCAGAAGGTGCTCGACA
GTCGGCGCATTGCCATCGCGGCCTCCCACGAGTTTGGTGTACCGCTCCTGGACTTGAGCGCGCTGGAACTGGTCGACATG
CCGCTGAATCTGGTCGACGAGAAGCTGATCCGCAAGCACCGGGCACTGCCCTTGTTCCGTCGCGGCAACCGCATCTTCCT
CGCCCTGTCCGACCCGACCAATGGTCAGGCCTTGGAGGAAATTCGTTTCAACACCGGGCTCGCCACGGACGCGGTCCTGG
TGGAGGAGGACAAACTCGCGGCGGCCATCGATCGTGCCATCGATGCCCAGGACACCACCATGACGGACCTCATGGATACG
GACCTGGACAACCTCGATATCGCCGCGGACGACGAGGACCAACACGACGACTCCGATCTCAATGTCGATGATGCACCGGT
GGTGCGCTACGTCAACAAGATCCTGGTGGACGCCATCACCAACAGTGCCTCGGACATCCACTTCGAGCCCTATGAAAAGT
ACTTCCGCATCCGTTTTCGTCAGGACGGTCTGCTGCGCGAGGTCGCCAACCCGCCGCTCAACATCGCCGCGCGTCTGGTC
GCCCGGCTCAAGGTCATGTCGCGGATGAACATCGCCGAGCGCCGGGTCCCGCAGGACGGGCGCATCAAGCTCAAGCTCAG
CCGCACCCGTGATATCGACTTCCGCGTCAACACCCTGCCGTGCCTGCACGGGGAGAAGGTGGTGCTGCGGATTCTGGACT
CCACCTCGGCCCAGGTCGGGATCGATGCGCTGGGCTTCGAGCCGGAGCAGCGCGACCTCTTCCTCGCCGCCATCAAGAAG
CCCTACGGGATGATCCTGGTCACGGGCCCCACGGGCTCCGGCAAGACCGTTTCCCTCTATAGCGCGATCAACATCCTCAA
CGCGCCGGATGTCAACATCTCAACGGTCGAGGACCCGGTAGAGATTCAGGTGGCGGGGATCAACCAGGTCAACATGAACC
CAAAAACGGGGCTGACCTTCGCCTCCGCGCTACGCGCCTTTCTCCGCCAGGATCCGGACATCCTGATGGTCGGGGAGATC
CGCGACCTGGAGACCGCCGAGATCGCGGTCAAGGCCGCCCAGACCGGACACATGGTGCTCTCCACCCTGCACACCAACGA
CGCCCCACAGACGCTCACCCGACTGGCCAATATGGGTGTGGCGCCGTTCAACATTGCCTCCTCGATCCTGCTGATCATGG
CCCAGCGCCTGACCCGTAAATTGTGCCCAAGCTGCAAGCAGCCCGAGGAGGTACCGCGCGAGGCCCTGCGCGAGGAGGGG
TTCACGGAAGCCGATCTCGACGAGGGCATCACCGTCTACCGCCCGGTCGGCTGCGAACGCTGCACCAAGGGCTATAAGGG
ACGAATCGGGATTTTTCAGGTCATGAAGGTATCCGAGGCGATCGGGCGCCTCATCATGGAGGGCGGCAACGCGCTCCAGT
TGGCCGAACAGGCAGCGCGCGAAGGGGTCGCAAACTTGCGGCAATCCGGGCTGCGCAAGGTCAAGGCCGGAATCACCAGC
CTTGAAGAGATCAACCGAGTCACGAAGGACTGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(193-460)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-151)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A2K8UFQ6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Legionella pneumophila strain ERS1305867

55.304

100

0.558
  pilB Acinetobacter baumannii D1279779

54.878

100

0.553
  pilB Acinetobacter baylyi ADP1

54.816

100

0.549
  pilF Neisseria gonorrhoeae MS11

49.293

99.298

0.489
  pilB Vibrio cholerae strain A1552

49.635

96.14

0.477
  pilB Vibrio parahaemolyticus RIMD 2210633

50.284

92.807

0.467
  pilB Vibrio campbellii strain DS40M4

49.62

92.281

0.458
  pilB/pilB1 Synechocystis sp. PCC 6803

40.962

91.228

0.374
  pilF Thermus thermophilus HB27

39.888

93.684

0.374

Multiple sequence alignment