Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   BVH73_RS05610 Genome accession   NZ_CP020046
Coordinates   1200449..1202188 (+) Length   579 a.a.
NCBI ID   WP_079416849.1    Uniprot ID   -
Organism   Thiomonas intermedia strain ATCC 15466     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1195449..1207188
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BVH73_RS05580 proB 1195850..1196974 (-) 1125 WP_179947972.1 glutamate 5-kinase -
  BVH73_RS05585 cgtA 1196982..1198058 (-) 1077 WP_079416841.1 Obg family GTPase CgtA -
  BVH73_RS05590 rpmA 1198197..1198454 (-) 258 WP_079416843.1 50S ribosomal protein L27 -
  BVH73_RS05595 rplU 1198467..1198778 (-) 312 WP_079416845.1 50S ribosomal protein L21 -
  BVH73_RS05600 - 1199110..1200120 (+) 1011 WP_079416847.1 polyprenyl synthetase family protein -
  BVH73_RS05610 pilB 1200449..1202188 (+) 1740 WP_079416849.1 type IV-A pilus assembly ATPase PilB Machinery gene
  BVH73_RS05615 pilC 1202203..1203423 (+) 1221 WP_079416852.1 type II secretion system F family protein Machinery gene
  BVH73_RS05620 pilD 1203423..1204292 (+) 870 WP_079416855.1 A24 family peptidase Machinery gene
  BVH73_RS05625 coaE 1204289..1204909 (+) 621 WP_079416858.1 dephospho-CoA kinase -
  BVH73_RS05630 zapD 1205021..1205773 (+) 753 WP_079416861.1 cell division protein ZapD -
  BVH73_RS05635 - 1205780..1206031 (+) 252 WP_079416863.1 DNA gyrase inhibitor YacG -
  BVH73_RS05640 - 1206056..1206472 (-) 417 WP_079416865.1 NUDIX domain-containing protein -

Sequence

Protein


Download         Length: 579 a.a.        Molecular weight: 63271.51 Da        Isoelectric Point: 5.7500

>NTDB_id=220667 BVH73_RS05610 WP_079416849.1 1200449..1202188(+) (pilB) [Thiomonas intermedia strain ATCC 15466]
MSATSIAASASPPSKALPGLAHALVQAELLPRTLVEQIHQRSQETRSSFIATLIGSGSMNAPDLAHWVSKTFSLPLLDLD
AYDPTRLATGAIDSKIIQSYKVLPLLKRGNRLVVATADPADHEAEDKIKFQSQAQIELVVVEYDKLTRIIEQAGQSANSQ
ISALVGEDFDLGDLSDGSEAPEAKEQANEVDDAPVVRFLQKMLIDAINMRASDLHFEPFEHFYRIRFRVDGELREIAQPP
ASIKEKLASRIKVISKLDIAERRVPQDGRMKLRISPEKSIDFRVSTLPTLYGEKIVMRILDSSAAKLGIDALGYEPEERE
KLLRAIRRPYGMILVTGPTGSGKTVSLYTCLNLLNQPGVNIATAEDPAEINLPGVNQVNVNEKAGLTFANALRAFLRQDP
DIIMVGEIRDLETADISIKAAQTGHLVLSTVHTNDAPTTLTRLMNMGVPAFNIASSVILITAQRLARKLCNQCKTPVDLP
AETLLEAGFQEEDLDGSWKPYKAGKCNACGGSGYKGRVGIYQVMPISEEIQRIILNHGTAMDIAEQARKEGVRSLRESGL
IKVKQGVTSLEEIIAVSNE

Nucleotide


Download         Length: 1740 bp        

>NTDB_id=220667 BVH73_RS05610 WP_079416849.1 1200449..1202188(+) (pilB) [Thiomonas intermedia strain ATCC 15466]
ATGTCCGCCACCTCGATCGCCGCATCCGCCTCACCGCCATCCAAGGCCCTGCCTGGCCTCGCGCATGCTTTGGTGCAGGC
TGAACTGCTGCCGCGCACCCTGGTTGAGCAGATCCATCAGCGCAGTCAGGAAACGCGCAGCAGCTTCATCGCCACGCTCA
TTGGCAGTGGCTCCATGAATGCGCCCGATCTGGCGCATTGGGTGAGCAAGACCTTTTCCTTGCCGCTTCTCGACCTGGAC
GCCTATGACCCGACCCGTCTGGCAACCGGTGCGATCGACTCCAAAATCATTCAGAGCTACAAGGTGCTGCCGCTGCTCAA
GCGCGGCAATCGCCTGGTCGTGGCCACCGCAGATCCCGCCGATCACGAGGCAGAGGACAAGATCAAGTTTCAGTCGCAGG
CACAGATCGAGCTGGTCGTCGTCGAGTATGACAAGCTGACGCGCATCATCGAGCAGGCTGGGCAGTCGGCCAACAGCCAG
ATTTCGGCGCTGGTCGGCGAAGATTTCGATCTGGGCGACCTTAGCGATGGCAGCGAGGCCCCGGAGGCCAAGGAACAGGC
CAACGAGGTTGACGATGCTCCCGTGGTGCGCTTTCTGCAGAAGATGCTGATTGATGCCATCAATATGCGGGCATCCGACC
TGCATTTCGAGCCTTTCGAGCACTTCTACCGGATCCGGTTTCGCGTCGACGGCGAACTACGCGAAATTGCCCAGCCGCCA
GCCTCGATCAAAGAGAAGCTCGCCTCGCGCATCAAGGTGATTTCCAAGCTCGACATTGCCGAGCGCCGGGTGCCCCAGGA
TGGGCGCATGAAGCTGCGAATCAGCCCCGAGAAATCCATCGACTTTCGCGTCAGCACGCTACCCACCCTTTACGGCGAGA
AGATCGTGATGCGGATTCTCGACTCGTCCGCAGCCAAGCTCGGCATCGACGCGCTGGGTTACGAGCCCGAGGAACGCGAA
AAGCTTCTGCGGGCAATTCGGCGCCCCTACGGCATGATTCTGGTCACCGGGCCGACGGGCAGCGGCAAGACCGTTTCGCT
CTACACCTGTCTGAATCTGCTCAACCAACCCGGGGTCAACATCGCCACGGCTGAAGATCCCGCAGAAATCAACCTGCCCG
GCGTCAACCAGGTCAACGTCAATGAGAAGGCCGGCCTGACCTTCGCCAACGCGCTGCGCGCCTTCCTTCGCCAGGATCCG
GACATCATCATGGTCGGGGAAATCCGCGATCTGGAAACCGCCGACATCTCGATCAAGGCAGCGCAAACCGGCCACCTGGT
GCTGTCCACGGTGCACACCAACGACGCACCCACCACGCTGACCCGCTTGATGAACATGGGCGTTCCCGCCTTCAACATCG
CCTCCAGCGTCATCCTCATCACGGCGCAGCGGTTGGCACGCAAGCTCTGCAATCAGTGCAAGACACCGGTTGACCTGCCT
GCCGAAACCCTGCTTGAGGCGGGCTTCCAGGAAGAAGATCTCGACGGCAGCTGGAAGCCTTACAAGGCCGGAAAATGCAA
CGCCTGCGGCGGCTCGGGCTACAAGGGACGTGTCGGCATCTATCAGGTCATGCCGATTTCCGAGGAAATACAACGCATCA
TCCTTAATCACGGCACAGCCATGGACATCGCAGAGCAGGCGCGCAAGGAAGGCGTGCGCTCGCTGCGCGAGTCCGGGTTG
ATCAAGGTGAAACAGGGGGTCACCTCCCTGGAAGAAATCATCGCCGTGTCCAACGAGTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(57-157)

Type II/IV secretion system protein (T2SSE)

(201-468)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

52.778

99.482

0.525
  pilB Acinetobacter baumannii D1279779

52.797

98.791

0.522
  pilF Neisseria gonorrhoeae MS11

52.753

97.237

0.513
  pilB Legionella pneumophila strain ERS1305867

50.802

96.891

0.492
  pilB Vibrio cholerae strain A1552

47.872

97.409

0.466
  pilB Vibrio parahaemolyticus RIMD 2210633

46.595

96.373

0.449
  pilB Vibrio campbellii strain DS40M4

46.071

96.718

0.446
  pilB/pilB1 Synechocystis sp. PCC 6803

41.346

89.81

0.371
  pilF Thermus thermophilus HB27

38.838

95.164

0.37

Multiple sequence alignment