Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   BVC89_RS09645 Genome accession   NZ_CP020038
Coordinates   2275283..2276992 (-) Length   569 a.a.
NCBI ID   WP_086930996.1    Uniprot ID   -
Organism   Agarilytica rhodophyticola strain 017     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2270283..2281992
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BVC89_RS09615 - 2270394..2271143 (+) 750 WP_086930990.1 hypothetical protein -
  BVC89_RS09620 pilE 2271314..2271826 (-) 513 WP_086930991.1 pilin Machinery gene
  BVC89_RS09625 - 2272215..2272406 (-) 192 WP_086930992.1 DNA gyrase inhibitor YacG -
  BVC89_RS09630 coaE 2272413..2273021 (-) 609 WP_086930993.1 dephospho-CoA kinase -
  BVC89_RS09635 pilD 2273025..2273918 (-) 894 WP_086930994.1 A24 family peptidase Machinery gene
  BVC89_RS09640 pilC 2274065..2275276 (-) 1212 WP_086930995.1 type II secretion system F family protein Machinery gene
  BVC89_RS09645 pilB 2275283..2276992 (-) 1710 WP_086930996.1 type IV-A pilus assembly ATPase PilB Machinery gene
  BVC89_RS09650 - 2277564..2277656 (+) 93 Protein_1872 prepilin-type N-terminal cleavage/methylation domain-containing protein -
  BVC89_RS09655 - 2278023..2278997 (+) 975 WP_086930997.1 NADPH:quinone oxidoreductase family protein -
  BVC89_RS09660 nadC 2279112..2279954 (-) 843 WP_086930998.1 carboxylating nicotinate-nucleotide diphosphorylase -
  BVC89_RS09665 - 2279959..2280468 (-) 510 WP_086930999.1 TIGR02281 family clan AA aspartic protease -

Sequence

Protein


Download         Length: 569 a.a.        Molecular weight: 62216.12 Da        Isoelectric Point: 4.8677

>NTDB_id=220621 BVC89_RS09645 WP_086930996.1 2275283..2276992(-) (pilB) [Agarilytica rhodophyticola strain 017]
MMLSGLARRLVRDELLTQEDAVKAVETAAKEKIPLPFYLVSNSDISSSAIAGAASDEFGTPLIDMQSLDHSLLPNGLVDI
KLIQKHRVLPIFKRGTRLYVAVSDPTNLHALDEIKFNTGLTTEALLTHADKLQSAVEKYLNAEEESLGDALGGLDGDDLD
GLEVEAVSDDSQEKDDNEVDEAPIVRFVNKVLLDAIKTGASDIHFEPYEKAYRVRFRTDGILKEVARPPVNLAIKLAARL
KVMSQMDISERRVPQDGRIKMKISKTRAIDFRVNTLPTLFGEKVVLRILDPSSAKLGIDALGYEDSQKAMFLDALQQPQG
MILVTGPTGSGKTVSLYTGLNILNTIERNISTAEDPVEINLEGINQVNVNTKVGLTFASALRSFLRQDPDIVMVGEIRDL
ETAEIGIKAAQTGHLVLSTLHTNSAAETLTRLLNMGVPTFNVATTVSLIIAQRLARRLCNSCKLPADDIPEEVFKEEGFD
DIGMTRDEITLFRPNGCEKCNEGYKGRVGVYEVVRITPEISRIIMEGGNSIQIADAAKASGFNNLRVSALRKAAMGTTSL
EEANRVTKD

Nucleotide


Download         Length: 1710 bp        

>NTDB_id=220621 BVC89_RS09645 WP_086930996.1 2275283..2276992(-) (pilB) [Agarilytica rhodophyticola strain 017]
ATGATGTTAAGTGGACTGGCTCGGCGGCTTGTCAGAGACGAGCTTCTTACTCAAGAAGATGCTGTAAAAGCCGTAGAAAC
GGCTGCCAAAGAAAAAATCCCCCTGCCCTTCTATCTGGTGAGCAATTCCGACATCAGCTCCAGCGCTATAGCAGGTGCAG
CATCTGACGAGTTTGGTACCCCGCTTATCGACATGCAGTCGCTGGATCATAGTCTTCTCCCCAATGGCTTGGTGGATATC
AAGTTGATCCAAAAACACCGGGTGTTGCCCATTTTTAAACGTGGCACACGCTTGTACGTTGCTGTATCAGACCCGACTAA
CCTCCACGCCCTGGATGAAATCAAATTTAACACCGGCTTAACAACTGAAGCTCTGCTTACCCATGCCGACAAACTGCAAA
GTGCTGTCGAAAAATACTTAAATGCAGAAGAGGAAAGCTTAGGTGATGCCCTAGGTGGCCTGGATGGAGACGATCTCGAT
GGACTCGAAGTTGAAGCCGTAAGTGATGACTCTCAAGAAAAAGACGACAATGAAGTAGACGAAGCACCTATTGTACGCTT
TGTTAACAAAGTGCTTTTGGATGCGATTAAAACTGGCGCTTCTGATATTCACTTTGAGCCTTATGAGAAAGCTTACCGTG
TAAGATTTCGAACCGATGGAATATTGAAAGAAGTGGCCCGTCCTCCAGTAAATTTGGCGATCAAGCTAGCGGCACGCTTA
AAAGTTATGTCACAAATGGACATTTCTGAGCGCCGGGTGCCACAGGATGGACGTATCAAGATGAAGATTTCCAAGACGCG
GGCAATTGATTTTCGCGTTAACACATTACCGACCTTGTTCGGCGAGAAAGTGGTACTTCGAATTCTCGATCCTTCTAGTG
CAAAATTGGGCATCGACGCTCTCGGTTATGAAGATAGTCAAAAAGCAATGTTTTTAGACGCTCTTCAACAGCCGCAAGGT
ATGATTCTTGTGACAGGGCCTACAGGATCAGGAAAAACCGTATCCCTTTATACCGGCTTAAATATCTTAAACACCATAGA
GCGCAATATTTCTACCGCCGAAGATCCAGTAGAAATTAACCTGGAAGGCATTAACCAGGTGAATGTTAATACAAAAGTAG
GCTTAACCTTTGCATCAGCACTTCGTTCATTTTTGCGGCAAGATCCTGATATTGTCATGGTAGGTGAGATTCGAGATTTA
GAAACCGCCGAGATTGGCATTAAGGCTGCTCAAACAGGTCACTTAGTGCTCTCAACCTTACATACTAACAGTGCCGCAGA
AACACTCACGCGCTTGTTAAATATGGGGGTACCCACATTTAATGTGGCAACAACGGTAAGTTTAATTATTGCCCAGCGCC
TTGCCAGAAGACTATGCAATAGCTGTAAACTACCGGCTGATGATATTCCTGAAGAAGTGTTTAAAGAGGAAGGTTTCGAT
GATATCGGTATGACGCGAGATGAAATCACACTATTCCGACCAAACGGCTGTGAGAAGTGTAATGAAGGTTATAAGGGACG
TGTTGGTGTGTATGAAGTGGTTCGCATTACTCCGGAAATATCACGCATTATAATGGAAGGAGGTAACTCCATTCAGATCG
CTGATGCCGCTAAGGCATCAGGGTTTAATAACTTAAGAGTCTCGGCCTTACGCAAAGCGGCGATGGGTACGACGAGTTTA
GAAGAAGCAAATCGGGTAACGAAGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(190-457)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(39-145)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baylyi ADP1

60.739

99.824

0.606
  pilB Acinetobacter baumannii D1279779

60.563

99.824

0.605
  pilB Legionella pneumophila strain ERS1305867

57.118

100

0.571
  pilB Vibrio cholerae strain A1552

51.494

100

0.515
  pilB Vibrio campbellii strain DS40M4

49.824

99.824

0.497
  pilB Vibrio parahaemolyticus RIMD 2210633

50

99.473

0.497
  pilF Neisseria gonorrhoeae MS11

48.522

100

0.49
  pilB/pilB1 Synechocystis sp. PCC 6803

41.304

88.928

0.367

Multiple sequence alignment