Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   OTEC02_RS17115 Genome accession   NZ_CP020015
Coordinates   3611950..3613662 (+) Length   570 a.a.
NCBI ID   WP_081175451.1    Uniprot ID   -
Organism   Acinetobacter lactucae strain OTEC-02     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3606950..3618662
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  OTEC02_RS17080 (OTEC02_17080) nusA 3607838..3609322 (-) 1485 WP_002115134.1 transcription termination factor NusA -
  OTEC02_RS17085 (OTEC02_17085) rimP 3609360..3609884 (-) 525 WP_016144169.1 ribosome maturation factor RimP -
  OTEC02_RS17105 (OTEC02_17105) secG 3610522..3610851 (-) 330 WP_002115012.1 preprotein translocase subunit SecG -
  OTEC02_RS17110 (OTEC02_17110) tpiA 3610864..3611658 (-) 795 WP_057104882.1 triose-phosphate isomerase -
  OTEC02_RS17115 (OTEC02_17115) pilB 3611950..3613662 (+) 1713 WP_081175451.1 type IV-A pilus assembly ATPase PilB Machinery gene
  OTEC02_RS17120 (OTEC02_17120) pilC 3613690..3614916 (+) 1227 WP_016144166.1 type II secretion system F family protein Machinery gene
  OTEC02_RS19330 (OTEC02_17125) pilD 3614916..3615776 (+) 861 WP_016144165.1 prepilin peptidase Machinery gene
  OTEC02_RS17130 (OTEC02_17130) coaE 3615778..3616374 (+) 597 WP_081175453.1 dephospho-CoA kinase -
  OTEC02_RS17135 (OTEC02_17135) - 3616371..3617285 (-) 915 WP_081175455.1 DMT family transporter -
  OTEC02_RS17140 (OTEC02_17140) rlmB 3617321..3618070 (-) 750 WP_005079348.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  OTEC02_RS17145 (OTEC02_17145) - 3618173..3618499 (-) 327 WP_002115102.1 pyrimidine/purine nucleoside phosphorylase -

Sequence

Protein


Download         Length: 570 a.a.        Molecular weight: 63650.10 Da        Isoelectric Point: 5.0932

>NTDB_id=220106 OTEC02_RS17115 WP_081175451.1 3611950..3613662(+) (pilB) [Acinetobacter lactucae strain OTEC-02]
MSVIHTSPKFSGFFRRLVEEKHVSAATMQTALDAAKRAKRDTVAYLIEEIHLSPSLLAETISIEFAEPYFDLDVYDTNQI
PKDLVDQKLILKHRVLPLIQRGQILYVATSNPSNIEAIDAIRFNSKLMVEPVIVEHHKLEKILTQQFTEESSFEFSDEEF
DLDVDLEAPPSQEDEEETPQGDEAPIVKYINKLLIDAIRMGASDLHFEPYEKSYRVRYRVDGVLRQIANPPLQLANRLAS
RLKVMSQMDISEKRVPQDGRIKLKLSKTKAIDFRVNSLPTLFGEKLVLRILDPSSAMLGIDALGYEEDQKALFLEALDKP
QGMLLITGPTGSGKTVSLYTGLNILNTESSNISTAEDPVEINLEGINQVNVNPKVGLTFSAALKSFLRQDPDIIMVGEIR
DLETAEIAIKAAQTGHMVMSTLHTNSAPETLTRLRNMGVPSFNIATSVNLVIAQRLARRLCPQCKIPADIPKQSLLEMGF
TEQDLTHPDFQVFQPVGCTECREGYKGRVGIYEVMKVTPEISKIIMEDGNALEIAAASEKLGFNNLRRSGLKKVMQGVTS
LQEVNRVTSE

Nucleotide


Download         Length: 1713 bp        

>NTDB_id=220106 OTEC02_RS17115 WP_081175451.1 3611950..3613662(+) (pilB) [Acinetobacter lactucae strain OTEC-02]
ATGTCAGTCATACATACATCTCCAAAATTTTCGGGGTTTTTTCGGCGATTAGTCGAAGAAAAACATGTGTCGGCAGCGAC
CATGCAAACAGCATTAGATGCAGCAAAGAGAGCTAAACGGGATACGGTTGCGTACCTGATTGAAGAGATTCATCTCTCCC
CTTCTTTATTAGCTGAAACTATTTCTATTGAATTTGCTGAACCATATTTTGATCTGGACGTTTACGATACCAACCAAATT
CCTAAAGATTTGGTTGACCAAAAACTGATTTTAAAACATCGGGTCTTACCTCTTATACAAAGAGGACAGATTTTATATGT
CGCGACCAGCAATCCAAGTAATATTGAGGCGATTGATGCCATTCGCTTCAATAGTAAGCTCATGGTTGAACCAGTTATTG
TCGAGCACCATAAGCTTGAGAAAATTCTGACGCAACAATTTACCGAAGAGAGCAGTTTCGAGTTTAGTGATGAAGAGTTT
GATCTTGATGTAGACCTTGAAGCTCCACCATCACAAGAGGATGAGGAAGAAACACCTCAAGGCGATGAAGCACCTATTGT
TAAATATATTAATAAATTACTCATCGATGCGATTCGTATGGGAGCTTCAGACTTGCATTTTGAACCCTATGAAAAATCAT
ATCGGGTCCGTTACCGAGTCGACGGCGTGTTGCGTCAGATTGCAAATCCGCCTTTGCAATTGGCTAACCGTTTGGCTTCA
CGTTTAAAAGTCATGTCTCAAATGGATATTTCCGAAAAACGGGTGCCTCAAGATGGTCGTATCAAACTTAAGCTGTCAAA
AACTAAAGCAATCGATTTTCGTGTGAACTCACTTCCAACCTTGTTTGGTGAAAAACTGGTTCTGCGTATTCTCGATCCAT
CTAGCGCGATGCTCGGAATTGATGCTTTAGGATATGAAGAAGATCAGAAAGCTCTTTTTTTAGAAGCACTGGATAAGCCC
CAAGGTATGCTTCTAATTACAGGCCCTACTGGCTCTGGTAAAACGGTATCTTTATATACAGGCTTAAATATTTTAAATAC
CGAAAGCTCTAATATTTCCACTGCCGAAGATCCAGTCGAAATCAACCTTGAAGGTATTAATCAGGTTAACGTCAACCCGA
AAGTAGGTCTTACTTTTTCGGCCGCACTTAAATCATTTTTACGTCAGGACCCTGACATTATTATGGTTGGTGAGATTCGT
GACTTAGAAACGGCTGAAATTGCGATTAAAGCTGCGCAAACCGGTCACATGGTAATGTCCACACTGCATACCAATAGTGC
TCCAGAAACACTAACTCGCCTACGTAATATGGGCGTTCCATCCTTTAATATCGCAACTTCTGTCAATCTGGTGATTGCTC
AACGTTTGGCACGTCGGCTATGCCCACAGTGTAAAATACCTGCTGACATTCCTAAACAGAGCCTATTAGAAATGGGCTTT
ACCGAACAAGATTTAACCCATCCCGATTTTCAAGTTTTTCAACCTGTCGGTTGTACTGAGTGTCGGGAAGGCTATAAAGG
TCGAGTAGGTATTTACGAGGTAATGAAAGTTACACCTGAAATTTCCAAGATTATTATGGAAGATGGCAATGCTTTAGAAA
TCGCTGCTGCTTCTGAAAAACTGGGGTTTAATAATCTGCGTCGTTCGGGATTAAAGAAAGTCATGCAGGGTGTTACTTCT
TTACAGGAAGTAAACCGCGTGACCAGTGAATAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(192-459)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-145)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

95.088

100

0.951
  pilB Acinetobacter baylyi ADP1

78.246

100

0.782
  pilB Legionella pneumophila strain ERS1305867

51.675

99.474

0.514
  pilF Neisseria gonorrhoeae MS11

49.822

98.596

0.491
  pilB Vibrio parahaemolyticus RIMD 2210633

51.908

91.93

0.477
  pilB Vibrio campbellii strain DS40M4

50.958

91.579

0.467
  pilB Vibrio cholerae strain A1552

50.482

91.053

0.46
  pilB/pilB1 Synechocystis sp. PCC 6803

35.761

100

0.388
  pilF Thermus thermophilus HB27

36.833

98.596

0.363

Multiple sequence alignment