Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   BVZ63_RS01750 Genome accession   NZ_CP020014
Coordinates   373395..374798 (-) Length   467 a.a.
NCBI ID   WP_105197617.1    Uniprot ID   -
Organism   Haemophilus influenzae strain 11P6H     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 368395..379798
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BVZ63_RS01715 (BVZ63_357) - 368636..368842 (-) 207 WP_005631184.1 heavy-metal-associated domain-containing protein -
  BVZ63_RS01720 (BVZ63_358) - 368928..369134 (-) 207 WP_005631184.1 heavy-metal-associated domain-containing protein -
  BVZ63_RS01725 (BVZ63_359) cueR 369211..369597 (+) 387 WP_005654127.1 Cu(I)-responsive transcriptional regulator -
  BVZ63_RS01730 (BVZ63_360) metJ 369611..369928 (-) 318 WP_005631186.1 met regulon transcriptional regulator MetJ -
  BVZ63_RS01735 (BVZ63_361) rho 370176..371438 (+) 1263 WP_005648966.1 transcription termination factor Rho -
  BVZ63_RS01740 (BVZ63_362) pilD 371492..372184 (-) 693 WP_005686720.1 prepilin peptidase Machinery gene
  BVZ63_RS01745 (BVZ63_363) pilC 372181..373398 (-) 1218 WP_005686722.1 type II secretion system F family protein Machinery gene
  BVZ63_RS01750 (BVZ63_364) pilB 373395..374798 (-) 1404 WP_105197617.1 GspE/PulE family protein Machinery gene
  BVZ63_RS01755 (BVZ63_365) pilA 374795..375244 (-) 450 WP_005686726.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  BVZ63_RS01760 (BVZ63_366) ampD 375359..375919 (+) 561 WP_005648974.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  BVZ63_RS01765 (BVZ63_368) corC 376542..377441 (+) 900 WP_011961742.1 CNNM family magnesium/cobalt transport protein CorC -
  BVZ63_RS01770 (BVZ63_369) lnt 377464..378993 (+) 1530 WP_074032781.1 apolipoprotein N-acyltransferase -
  BVZ63_RS01775 (BVZ63_370) rsmE 379043..379780 (+) 738 WP_005686736.1 16S rRNA (uracil(1498)-N(3))-methyltransferase -

Sequence

Protein


Download         Length: 467 a.a.        Molecular weight: 53319.62 Da        Isoelectric Point: 5.3433

>NTDB_id=220040 BVZ63_RS01750 WP_105197617.1 373395..374798(-) (pilB) [Haemophilus influenzae strain 11P6H]
MTSYALLHTQRVTAQNGEIFTISSDLWARNQQQQSLLLRYFALPLKEENNRLWLGVDSLSNLSACETISFITGKPVEPIL
LESSQLKELLQQLTPHQMQEEEEEQVKYYQHQEQSHLEQQDDEPVIRLLNQIFESALQKNASDIHLETLADQFQVRFRID
GVLQPQPLISKIFANRIISRLKLLAKLDISENRLPQDGRFQFKTTFSDILDFRLSTLPTHWGEKIVLRAQQNKPVELSFA
ELGMTENQQQAFQRALSQPQGLILVTGPTGSGKSISLYTALQWLNTPDKHIMTAEDPIEIELDGIIQSQINPQIGLDFSR
LLRAFLRQDPDIIMLGEIRDEESARIALRAAQTGHLVLSTLHTNDAISAISRLQQLGIQQYEIENSLLLVIAQRLVRKLC
SKCGGNLINSCDCHQGYRGRIGVYQFLHWQQNGYQTDFENLRESGLEKVSQGITDEKEIERVLGKNL

Nucleotide


Download         Length: 1404 bp        

>NTDB_id=220040 BVZ63_RS01750 WP_105197617.1 373395..374798(-) (pilB) [Haemophilus influenzae strain 11P6H]
ATGACGAGTTATGCTTTACTTCATACTCAGCGTGTAACCGCTCAAAATGGCGAGATCTTTACGATCTCGTCTGATTTATG
GGCGCGTAATCAACAACAGCAATCCTTGCTCTTGCGGTATTTTGCTTTGCCACTTAAAGAAGAAAATAATCGTCTTTGGC
TAGGGGTTGATTCTCTCTCCAATCTTTCAGCTTGTGAAACCATTTCGTTTATAACAGGAAAACCTGTCGAACCAATTTTG
TTAGAAAGCAGCCAACTCAAAGAACTGTTACAACAACTTACTCCGCACCAAATGCAAGAGGAAGAGGAAGAGCAAGTTAA
ATATTACCAACATCAAGAACAGTCTCATCTTGAACAACAAGATGATGAACCTGTTATCCGCTTACTTAATCAGATTTTTG
AATCTGCCTTACAAAAAAATGCCTCTGATATTCATTTAGAAACCTTGGCTGATCAGTTTCAAGTGCGGTTTAGAATTGAT
GGTGTTTTACAACCACAACCCTTAATAAGCAAAATATTCGCCAATCGTATTATTTCACGCTTAAAATTACTGGCTAAATT
AGATATTAGTGAAAATCGACTTCCACAAGATGGACGATTTCAATTTAAAACCACTTTTTCCGATATTCTTGATTTTCGCC
TTTCAACCTTACCAACCCATTGGGGCGAAAAAATCGTGTTGCGAGCGCAACAAAATAAACCTGTAGAACTTAGCTTTGCT
GAACTGGGTATGACCGAAAATCAGCAACAAGCATTTCAACGCGCACTTAGCCAGCCACAAGGATTAATTTTAGTAACTGG
CCCCACAGGAAGTGGGAAAAGTATCTCACTTTACACCGCACTTCAGTGGCTAAATACACCTGATAAACATATTATGACTG
CTGAGGATCCCATTGAAATTGAGCTTGATGGCATTATTCAAAGCCAAATTAATCCACAGATTGGATTAGATTTTAGCCGT
CTATTGCGCGCTTTTTTACGTCAAGATCCCGATATCATTATGCTAGGTGAAATTCGCGATGAAGAAAGTGCAAGGATTGC
ATTACGTGCCGCCCAAACGGGGCATTTGGTGCTTTCAACTTTACATACCAATGATGCAATTTCTGCCATTTCTCGTTTAC
AACAACTCGGTATTCAGCAGTATGAAATTGAAAACAGTTTACTACTTGTCATTGCACAACGTCTTGTACGAAAGCTTTGT
TCAAAGTGCGGTGGAAATTTAATAAATTCTTGTGATTGCCATCAAGGTTATCGAGGGCGAATCGGCGTGTATCAATTTCT
ACATTGGCAACAGAATGGCTATCAAACGGATTTTGAGAATTTACGAGAGAGTGGTTTAGAAAAAGTTAGCCAAGGCATAA
CAGATGAGAAAGAAATTGAACGTGTGTTAGGTAAAAACTTATGA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(131-398)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(32-98)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Haemophilus influenzae 86-028NP

96.781

99.786

0.966
  pilB Haemophilus influenzae Rd KW20

94.861

100

0.949
  pilB Glaesserella parasuis strain SC1401

58.297

98.073

0.572
  pilB Vibrio cholerae strain A1552

40.797

100

0.46
  pilB Vibrio campbellii strain DS40M4

40.695

100

0.426
  pilB Vibrio parahaemolyticus RIMD 2210633

40.373

100

0.418
  pilB Legionella pneumophila strain ERS1305867

39.673

100

0.415
  pilB Acinetobacter baylyi ADP1

38.855

100

0.407
  pilB Acinetobacter baumannii D1279779

39.256

100

0.407
  pilF Neisseria gonorrhoeae MS11

37.657

100

0.385
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

37.953

100

0.381
  pilF Thermus thermophilus HB27

36.421

100

0.37

Multiple sequence alignment