Detailed information NTG0219744

Bioinformatically predicted

Overview

• Name: pilM
• Type: Machinery gene
• Locus tag: BUM88_RS17970
• Genome accession: NZ_CP020000
• Coordinates: 3725427..3726485 (-)
• Length: 352 a.a.
• NCBI ID: WP_213033728.1
• Uniprot ID: -
• Organism: Acinetobacter calcoaceticus strain CA16
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 3720427..3731485

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
BUM88_RS17945 (BUM88_17945)	aroK	3720705..3721274 (-)	570	WP_003653807.1	shikimate kinase AroK	-
BUM88_RS17950 (BUM88_17950)	pilQ	3721286..3723439 (-)	2154	WP_080027891.1	type IV pilus secretin PilQ family protein	Machinery gene
BUM88_RS17955 (BUM88_17955)	pilP	3723502..3724029 (-)	528	WP_003653811.1	pilus assembly protein PilP	Machinery gene
BUM88_RS17960 (BUM88_17960)	pilO	3724040..3724789 (-)	750	WP_005038271.1	type 4a pilus biogenesis protein PilO	Machinery gene
BUM88_RS17965 (BUM88_17965)	pilN	3724786..3725427 (-)	642	WP_080027892.1	PilN domain-containing protein	Machinery gene
BUM88_RS17970 (BUM88_17970)	pilM	3725427..3726485 (-)	1059	WP_213033728.1	pilus assembly protein PilM	Machinery gene
BUM88_RS17975 (BUM88_17975)	ponA	3726647..3729208 (+)	2562	WP_155120919.1	penicillin-binding protein PBP1a	-
BUM88_RS17980 (BUM88_17980)	-	3729262..3730086 (-)	825	WP_080027894.1	putative RNA methyltransferase	-
BUM88_RS17985 (BUM88_17985)	-	3730089..3730421 (-)	333	WP_003653820.1	DUF7709 family protein	-

Sequence

Protein

• Length: 352 a.a.
• Molecular weight: 38935.51 Da
• Isoelectric Point: 4.4217

>NTDB_id=219744 BUM88_RS17970 WP_213033728.1 3725427..3726485(-) (pilM) [Acinetobacter calcoaceticus strain CA16]
MLRLYRKPNKGLMGVDISSTSVKLLELSVKNGKYLVESYALMPLPENSVVEKNILNPEAVAEALERAMNLANPQTSNAAI
AVPTSTVIHKTIEMDADMNDDEREVQIRLDAEQYIPFPLDEVSLDFEVLPDRLPNPNRVNVLLVATRTENVETRVEVLEL
VDLTPKLADVESYAVERAFSVFADSLPMGANTIGILDIGHTMTTLSVMQSGKIIYTREQVFGGKQLTLEIQSRYGLSSEE
AGRAKKERSLPDDYDIEVLEPFLDAVVQQAARSLQFFFSSSQFNEIDHILLAGGNANIPGLAKLLQQKLGYRVTIANPFL
QMGFSPQVDVQKIENDASSLMVACGLALRSFD

Nucleotide

• Length: 1059 bp

>NTDB_id=219744 BUM88_RS17970 WP_213033728.1 3725427..3726485(-) (pilM) [Acinetobacter calcoaceticus strain CA16]
GTGCTCAGGTTATATCGTAAACCGAATAAGGGGTTAATGGGTGTCGATATTAGTTCGACTTCTGTTAAGTTGTTAGAGCT
CTCTGTTAAGAACGGTAAATATTTGGTGGAAAGCTATGCTTTGATGCCTTTACCCGAGAACAGTGTAGTTGAAAAAAATA
TCTTGAATCCAGAGGCAGTTGCAGAAGCTTTGGAACGGGCGATGAATTTAGCAAATCCCCAAACCAGTAACGCTGCAATT
GCTGTTCCAACATCGACGGTTATTCATAAAACCATCGAAATGGATGCAGATATGAATGATGATGAGCGAGAAGTTCAGAT
TCGATTAGATGCGGAGCAGTATATACCGTTCCCTTTAGATGAAGTGAGCCTTGATTTTGAGGTTTTACCAGATCGACTGC
CTAATCCAAACCGCGTTAATGTACTTTTAGTTGCAACAAGAACAGAAAATGTTGAAACACGAGTAGAAGTGCTGGAACTG
GTGGACTTAACTCCTAAGCTTGCTGATGTAGAAAGTTATGCTGTCGAGCGTGCTTTTAGTGTATTTGCTGATAGCTTGCC
AATGGGTGCAAATACCATTGGTATTTTAGATATCGGCCATACCATGACCACATTATCGGTTATGCAAAGTGGCAAAATCA
TTTATACCCGTGAACAAGTCTTCGGTGGCAAACAGCTGACTTTGGAAATTCAAAGCCGTTATGGTTTGTCTTCAGAAGAA
GCGGGACGGGCTAAAAAAGAGCGCTCTTTACCAGATGATTATGATATTGAAGTCTTAGAGCCATTTCTTGATGCTGTCGT
GCAGCAAGCAGCTAGATCTCTACAATTTTTCTTCTCTTCATCACAATTCAATGAAATTGACCATATTTTACTCGCTGGTG
GGAATGCCAATATTCCTGGGCTTGCTAAATTGTTGCAGCAAAAACTTGGTTACCGTGTGACCATTGCCAATCCGTTTTTA
CAAATGGGTTTTTCTCCTCAAGTCGATGTTCAAAAAATTGAAAATGATGCCTCATCGTTAATGGTGGCATGTGGCTTGGC
TTTAAGGAGTTTTGATTAA

Domains

Predicted by InterproScan.

• Type IV pilus assembly protein PilM; (PilM_2) (14-351)

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilM	Acinetobacter baumannii D1279779	96.023	100	0.96
comM	Acinetobacter nosocomialis M2	96.023	100	0.96
comM	Acinetobacter baylyi ADP1	81.818	100	0.818
pilM	Legionella pneumophila strain ERS1305867	41.808	100	0.42