Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilO   Type   Machinery gene
Locus tag   BUM88_RS17960 Genome accession   NZ_CP020000
Coordinates   3724040..3724789 (-) Length   249 a.a.
NCBI ID   WP_005038271.1    Uniprot ID   A0AAW8QS31
Organism   Acinetobacter calcoaceticus strain CA16     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3719040..3729789
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BUM88_RS17940 (BUM88_17940) aroB 3719594..3720676 (-) 1083 WP_080027890.1 3-dehydroquinate synthase -
  BUM88_RS17945 (BUM88_17945) aroK 3720705..3721274 (-) 570 WP_003653807.1 shikimate kinase AroK -
  BUM88_RS17950 (BUM88_17950) pilQ 3721286..3723439 (-) 2154 WP_080027891.1 type IV pilus secretin PilQ family protein Machinery gene
  BUM88_RS17955 (BUM88_17955) pilP 3723502..3724029 (-) 528 WP_003653811.1 pilus assembly protein PilP Machinery gene
  BUM88_RS17960 (BUM88_17960) pilO 3724040..3724789 (-) 750 WP_005038271.1 type 4a pilus biogenesis protein PilO Machinery gene
  BUM88_RS17965 (BUM88_17965) pilN 3724786..3725427 (-) 642 WP_080027892.1 PilN domain-containing protein Machinery gene
  BUM88_RS17970 (BUM88_17970) pilM 3725427..3726485 (-) 1059 WP_213033728.1 pilus assembly protein PilM Machinery gene
  BUM88_RS17975 (BUM88_17975) ponA 3726647..3729208 (+) 2562 WP_155120919.1 penicillin-binding protein PBP1a -

Sequence

Protein


Download         Length: 249 a.a.        Molecular weight: 28118.99 Da        Isoelectric Point: 4.8667

>NTDB_id=219742 BUM88_RS17960 WP_005038271.1 3724040..3724789(-) (pilO) [Acinetobacter calcoaceticus strain CA16]
MSPDELHELSLEQAPSKKKKITLEKFLQQFNTLDMNSYGSWPLSVKITCWIFIFFAVLALGYFVVIQPKLQAIDNAQAQE
SNLLNEFREKDSKLRNLQQYQLQLQEMQANFNQQLEQLPKETEIPSLVEDINLTGVNSGLKFKNIRLEDEVKQEIFIEQP
ITMEATGDYHAFGAFVSSIAALPRIVTMHDFVVEAAPVKDSKSDIPVLNYSIKAKTYRYMGAVENAEHPENNASASVAPV
SSTANTQSK

Nucleotide


Download         Length: 750 bp        

>NTDB_id=219742 BUM88_RS17960 WP_005038271.1 3724040..3724789(-) (pilO) [Acinetobacter calcoaceticus strain CA16]
ATGAGTCCGGATGAACTTCATGAGTTGTCGTTAGAGCAAGCTCCATCTAAGAAAAAAAAGATCACTTTAGAAAAATTTCT
TCAGCAATTTAACACACTAGATATGAACAGCTATGGTAGTTGGCCATTATCTGTGAAAATTACTTGCTGGATTTTTATTT
TCTTTGCTGTGTTGGCTTTGGGATATTTTGTTGTTATTCAGCCAAAGCTACAAGCAATTGATAATGCACAGGCACAAGAA
AGTAATTTGTTGAATGAGTTTCGAGAAAAAGATTCAAAATTACGTAACTTGCAGCAATATCAGCTTCAACTTCAAGAGAT
GCAAGCAAACTTTAATCAGCAGTTAGAGCAACTGCCAAAAGAAACCGAAATTCCAAGCTTGGTTGAAGATATTAACTTAA
CTGGGGTGAATTCTGGCCTGAAGTTTAAAAATATCCGTTTAGAAGATGAAGTGAAGCAAGAAATCTTTATTGAACAGCCT
ATTACGATGGAAGCAACCGGCGATTATCACGCTTTTGGTGCTTTTGTCAGTAGTATTGCGGCATTACCACGTATTGTGAC
AATGCATGACTTTGTTGTAGAAGCTGCACCTGTTAAAGACAGTAAGTCAGATATTCCTGTACTTAATTATTCTATTAAAG
CAAAAACTTATCGCTATATGGGCGCCGTAGAAAATGCTGAACATCCGGAAAACAACGCTTCGGCTTCAGTAGCACCTGTT
TCCTCTACTGCTAATACACAGTCGAAATAG

Domains

Predicted by InterproScan.

Pilus assembly protein, PilO (PilO)

(73-220)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilO Acinetobacter baumannii D1279779

83.133

100

0.831
  comO Acinetobacter baylyi ADP1

69.758

99.598

0.695

Multiple sequence alignment