Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilF   Type   Machinery gene
Locus tag   ADP71_RS09500 Genome accession   NZ_CP019644
Coordinates   1924745..1926436 (-) Length   563 a.a.
NCBI ID   WP_019957099.1    Uniprot ID   -
Organism   Vitreoscilla sp. C1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1919745..1931436
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  ADP71_RS09475 (ADP71_25320) yacG 1921024..1921212 (-) 189 WP_104932064.1 DNA gyrase inhibitor YacG -
  ADP71_RS09480 (ADP71_25330) zapD 1921200..1921988 (-) 789 WP_019957103.1 cell division protein ZapD -
  ADP71_RS09485 (ADP71_25340) coaE 1921997..1922599 (-) 603 WP_019957102.1 dephospho-CoA kinase -
  ADP71_RS09490 (ADP71_25350) pilD 1922596..1923480 (-) 885 WP_019957101.1 A24 family peptidase Machinery gene
  ADP71_RS09495 (ADP71_25360) pilG 1923483..1924712 (-) 1230 WP_171461110.1 type II secretion system F family protein Machinery gene
  ADP71_RS09500 (ADP71_25370) pilF 1924745..1926436 (-) 1692 WP_019957099.1 type IV-A pilus assembly ATPase PilB Machinery gene
  ADP71_RS09505 (ADP71_25390) - 1926571..1927611 (-) 1041 WP_104932065.1 porin -
  ADP71_RS09510 (ADP71_25400) - 1928069..1928389 (+) 321 WP_171461111.1 helix-hairpin-helix domain-containing protein -
  ADP71_RS09515 (ADP71_25410) secA 1928439..1931117 (-) 2679 WP_171461112.1 preprotein translocase subunit SecA -

Sequence

Protein


Download         Length: 563 a.a.        Molecular weight: 61512.95 Da        Isoelectric Point: 5.0217

>NTDB_id=217510 ADP71_RS09500 WP_019957099.1 1924745..1926436(-) (pilF) [Vitreoscilla sp. C1]
MDSGLLRILAQQKIITDAQISAFESKAQADGRPIVAIVLQEKIITSQQLAHLLAHLFGCPLFDLSQFDQEKVDFSVITTE
QSAKFLTIPLLQRGRRLTVAVADPSVLQALQKATFSAGVTIDMVVVPADQLLGLIERLGASSSTNILDDFDTDLLGGGTI
GDEKEEEQVEDGPIAKFVHKVLLDALKAGASDIHFEFYEKVARVRLRVDGQLRELINPPIQIREQIASRIKVMSSLDISE
RRIPQDGRIKLKVSDTRAIDFRVSTLPTLFGEKIVMRILDSTAASLNVDQLGFEDFQKEILLDAINRPYGMVLVTGPTGS
GKTVSLYTCLNILNTPDVNISTAEDPAEINLPGINQVNVNDKQGLTFSAALKSFLRQDPDIIMVGEIRDLETADIAIKAA
QTGHMVFSTLHTNNAPATLSRMLNMGVAPFNIASSVNLIMAQRLLRRLCGECKAPVESPPDDVLKKMGFTDEMLTRMKTE
GLQFHGPVGCDRCRGGGYKGRLGVYELMPISSEIQRAIMKNATAVDIADIAYQEGMVDLRRAGLLKVMNGLTSLEEVVAG
TND

Nucleotide


Download         Length: 1692 bp        

>NTDB_id=217510 ADP71_RS09500 WP_019957099.1 1924745..1926436(-) (pilF) [Vitreoscilla sp. C1]
ATGGATAGCGGTTTATTACGTATATTGGCTCAACAAAAAATCATTACTGATGCGCAGATATCGGCTTTTGAAAGCAAAGC
TCAAGCTGATGGTAGACCTATTGTAGCGATTGTATTACAAGAAAAAATCATTACATCTCAACAATTGGCTCATTTGTTAG
CGCATTTATTTGGTTGCCCTTTGTTTGATTTAAGCCAGTTTGATCAAGAGAAAGTAGATTTCTCTGTGATTACTACTGAA
CAAAGTGCCAAGTTCTTAACTATACCTCTATTACAACGAGGACGTCGCTTGACCGTAGCAGTTGCAGACCCATCGGTATT
GCAAGCTTTGCAAAAAGCAACGTTCTCTGCTGGGGTAACCATTGATATGGTTGTGGTTCCAGCAGATCAATTATTAGGTT
TAATTGAACGTTTAGGTGCCAGCTCTTCTACCAATATTTTAGATGATTTTGATACTGATTTATTGGGTGGCGGAACGATT
GGGGATGAAAAAGAAGAAGAGCAAGTTGAAGATGGTCCGATTGCCAAATTTGTACATAAAGTGTTATTGGATGCATTGAA
AGCGGGTGCATCGGATATCCATTTCGAGTTTTATGAAAAAGTGGCACGTGTGCGCTTGCGTGTTGACGGACAATTGCGCG
AGTTGATTAATCCACCGATTCAAATCAGAGAGCAAATTGCTTCGCGTATCAAAGTTATGTCTTCTTTGGATATTTCAGAA
AGACGCATTCCTCAAGACGGCCGTATTAAATTGAAAGTCAGCGATACTCGTGCCATCGACTTTCGTGTGAGTACTTTGCC
AACGTTGTTTGGCGAAAAAATTGTAATGCGTATTTTGGACTCAACAGCGGCATCGCTGAATGTTGATCAATTGGGTTTTG
AGGATTTCCAGAAAGAAATCTTGTTAGATGCGATTAACCGTCCATATGGCATGGTGCTAGTAACAGGCCCAACAGGTTCG
GGTAAAACGGTTTCTTTGTATACTTGTTTGAACATTTTGAATACGCCTGATGTCAATATTTCTACTGCAGAGGATCCAGC
AGAGATTAACTTGCCTGGCATTAATCAGGTGAATGTGAATGACAAACAAGGTTTAACGTTCTCAGCGGCCTTAAAGTCTT
TTTTACGCCAAGATCCCGATATTATTATGGTGGGTGAAATCCGTGATTTGGAAACTGCCGATATTGCCATTAAGGCCGCC
CAAACTGGCCATATGGTATTTTCCACACTGCATACCAATAATGCGCCAGCAACTTTATCGCGCATGTTAAATATGGGTGT
GGCACCTTTTAATATTGCCAGTTCAGTGAATTTGATTATGGCGCAACGTCTGTTGCGTCGTTTGTGTGGCGAATGTAAAG
CACCTGTTGAATCTCCTCCAGATGATGTTTTGAAAAAAATGGGCTTCACTGATGAAATGTTGACCAGAATGAAAACTGAA
GGCTTGCAGTTTCATGGTCCTGTTGGGTGTGATCGTTGTCGTGGCGGTGGCTATAAAGGTCGTTTGGGTGTGTATGAATT
GATGCCGATTAGTAGTGAAATTCAGCGTGCTATTATGAAAAATGCGACTGCAGTTGATATTGCTGACATTGCTTATCAAG
AAGGTATGGTAGATTTACGCCGTGCAGGTTTGTTAAAAGTGATGAATGGATTGACTTCGTTGGAAGAAGTGGTTGCAGGC
ACTAACGATTAG

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(180-447)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(40-143)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilF Neisseria gonorrhoeae MS11

63.009

100

0.632
  pilB Acinetobacter baumannii D1279779

51.699

99.29

0.513
  pilB Acinetobacter baylyi ADP1

50.532

100

0.506
  pilB Legionella pneumophila strain ERS1305867

48.135

100

0.481
  pilB Vibrio cholerae strain A1552

46.726

100

0.469
  pilB Vibrio parahaemolyticus RIMD 2210633

45.487

100

0.456
  pilB Vibrio campbellii strain DS40M4

45.31

100

0.455
  pilB/pilB1 Synechocystis sp. PCC 6803

39.099

98.579

0.385
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.603

96.625

0.373
  pilF Thermus thermophilus HB27

37.319

98.046

0.366

Multiple sequence alignment