Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   BST95_RS17435 Genome accession   NZ_CP019450
Coordinates   3712413..3714128 (+) Length   571 a.a.
NCBI ID   WP_084200729.1    Uniprot ID   A0AAP8SNZ7
Organism   Halioglobus japonicus strain NBRC 107739     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3707413..3719128
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BST95_RS17420 (BST95_17425) - 3707522..3709411 (-) 1890 WP_084200727.1 hypothetical protein -
  BST95_RS17425 (BST95_17430) - 3709408..3711330 (-) 1923 WP_157114513.1 hypothetical protein -
  BST95_RS17430 (BST95_17435) - 3711495..3711986 (-) 492 WP_157114514.1 pilin -
  BST95_RS17435 (BST95_17440) pilB 3712413..3714128 (+) 1716 WP_084200729.1 type IV-A pilus assembly ATPase PilB Machinery gene
  BST95_RS17440 (BST95_17445) pilC 3714159..3715376 (+) 1218 WP_084200730.1 type II secretion system F family protein Machinery gene
  BST95_RS17445 (BST95_17450) pilD 3715407..3716270 (+) 864 WP_084200731.1 prepilin peptidase Machinery gene
  BST95_RS17450 (BST95_17455) coaE 3716267..3716857 (+) 591 WP_084200732.1 dephospho-CoA kinase -
  BST95_RS17455 (BST95_17460) mutT 3716849..3717244 (-) 396 WP_084200733.1 8-oxo-dGTP diphosphatase MutT -
  BST95_RS17460 (BST95_17465) argJ 3717245..3718471 (-) 1227 WP_084200734.1 bifunctional glutamate N-acetyltransferase/amino-acid acetyltransferase ArgJ -

Sequence

Protein


Download         Length: 571 a.a.        Molecular weight: 62626.27 Da        Isoelectric Point: 4.5912

>NTDB_id=215905 BST95_RS17435 WP_084200729.1 3712413..3714128(+) (pilB) [Halioglobus japonicus strain NBRC 107739]
MNDKAIGQLSGLAGRLVAEGVISAETATEAQRTASMEQMHLVQYLVEKQNVDGYRLAEVASQEFGVPQFDTECFDMAAMP
TGLVDVELVTKHHALPLYRRGNRLFIAVSDPTNLAALDEIKFHTGINTDAVLLEEATLSKLIANWVDMQDTLSDGLDDLD
ASDLDGIDVGTGESSDDDETSDVDETPIVRFVNKVLIDAIKQGASDIHFEPYENSYRVRFRTDGVLREMVKPPRNLSHRL
AARLKVMSQMDISERRVPQDGRIQMKLSRNRAIDFRVNTLPTLFGEKIVLRILDPTSAQLGIDALGYEDDQREMYLKALG
QPQGMILVTGPTGSGKTVSLYTGLNILNTAERNISTAEDPVEINLEGINQVHVNPKVGLNFAEALRSFLRQDPDIIMVGE
IRDLETAEIAIKAAQTGHLVLSTLHTNSAAETVTRLLNMGVPAFNVATSVDLIIAQRLARRLCKECAAPADDIPHDVLIK
QGFTEALLAEATILKAVGCSLCQDGYKGRVGVYEVVRITPPIARIIMEEGNSLQIQDQATQEGFNTLRVSALRKVAQGLI
SLEEANRITVD

Nucleotide


Download         Length: 1716 bp        

>NTDB_id=215905 BST95_RS17435 WP_084200729.1 3712413..3714128(+) (pilB) [Halioglobus japonicus strain NBRC 107739]
ATGAACGACAAAGCCATCGGGCAACTGAGTGGCCTTGCAGGTCGGCTTGTGGCCGAGGGGGTCATTTCGGCTGAAACCGC
CACAGAGGCTCAGCGCACTGCGTCTATGGAACAGATGCACCTCGTACAATATCTGGTTGAAAAACAGAATGTAGACGGTT
ACCGCCTGGCCGAGGTGGCATCCCAGGAGTTCGGTGTCCCTCAGTTCGACACCGAATGTTTCGATATGGCAGCCATGCCC
ACCGGTCTGGTGGACGTGGAACTGGTCACCAAACATCACGCCCTGCCGCTCTACCGGCGCGGCAATCGGTTGTTCATCGC
AGTCTCAGACCCCACCAACCTGGCGGCACTCGACGAAATCAAATTCCACACCGGCATTAACACAGATGCCGTGTTGCTGG
AGGAAGCCACCCTGAGCAAGTTGATTGCCAACTGGGTGGATATGCAGGACACCCTCTCCGATGGCCTGGATGACCTGGAC
GCGAGCGACCTCGACGGCATCGACGTAGGCACAGGCGAATCCAGTGACGACGACGAAACCAGCGACGTCGATGAAACGCC
TATCGTGCGCTTTGTAAACAAGGTGCTGATCGACGCCATCAAACAGGGTGCATCGGATATTCATTTCGAGCCCTACGAGA
ACAGCTATCGCGTGCGTTTTCGTACCGATGGCGTACTCAGGGAGATGGTCAAGCCACCGCGCAACCTGTCACACAGGCTG
GCGGCCCGCCTCAAGGTGATGTCGCAGATGGACATTTCCGAACGCCGGGTCCCCCAGGACGGGCGTATTCAGATGAAACT
GTCCCGCAATCGGGCCATCGACTTCCGTGTGAACACCCTGCCCACACTGTTTGGCGAGAAAATCGTACTGCGTATTCTCG
ACCCAACCAGCGCTCAGCTCGGTATTGATGCCCTCGGTTATGAGGACGATCAGCGCGAGATGTACCTTAAAGCCCTGGGA
CAACCGCAGGGCATGATCCTGGTCACCGGCCCCACGGGCTCCGGTAAGACAGTCTCGCTTTACACCGGCCTTAACATTCT
GAACACCGCCGAGCGCAATATCTCGACCGCCGAAGACCCGGTGGAGATTAACCTCGAGGGGATTAACCAGGTTCATGTGA
ACCCGAAGGTCGGCCTGAATTTTGCCGAGGCCCTGCGCTCCTTCCTGCGCCAGGACCCGGACATCATCATGGTGGGTGAG
ATTCGTGACCTCGAAACCGCCGAGATCGCGATTAAAGCCGCCCAGACCGGTCACCTGGTACTGTCGACGTTGCACACGAA
CAGCGCCGCGGAAACGGTAACCCGTTTGCTGAATATGGGCGTCCCCGCGTTTAACGTGGCGACGTCTGTTGATTTGATTA
TTGCCCAGCGTCTGGCCCGGCGGCTGTGCAAGGAATGCGCCGCGCCTGCGGATGATATTCCGCACGACGTGCTCATTAAA
CAGGGCTTTACCGAAGCACTGCTGGCCGAAGCGACCATTTTGAAAGCGGTTGGCTGCAGTCTGTGTCAGGATGGCTACAA
GGGCCGTGTCGGTGTGTATGAAGTCGTCAGGATTACACCACCTATTGCCAGGATCATCATGGAAGAAGGGAATTCACTGC
AAATCCAAGATCAGGCCACCCAGGAGGGCTTTAATACGCTCAGGGTTTCGGCACTGCGCAAGGTGGCCCAGGGGCTGATC
AGCCTGGAAGAGGCCAACCGGATTACCGTGGATTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(194-461)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(16-150)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

59.364

99.124

0.588
  pilB Acinetobacter baylyi ADP1

57.319

99.299

0.569
  pilB Legionella pneumophila strain ERS1305867

55.594

100

0.557
  pilB Vibrio cholerae strain A1552

52.93

92.644

0.49
  pilB Vibrio parahaemolyticus RIMD 2210633

53.372

90.893

0.485
  pilB Vibrio campbellii strain DS40M4

51.916

91.419

0.475
  pilF Neisseria gonorrhoeae MS11

47.518

98.774

0.469
  pilB/pilB1 Synechocystis sp. PCC 6803

39.746

96.497

0.384
  pilF Thermus thermophilus HB27

36.508

99.299

0.363
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.262

94.746

0.363

Multiple sequence alignment