Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilD   Type   Machinery gene
Locus tag   BST95_RS17445 Genome accession   NZ_CP019450
Coordinates   3715407..3716270 (+) Length   287 a.a.
NCBI ID   WP_084200731.1    Uniprot ID   A0AAP8MG27
Organism   Halioglobus japonicus strain NBRC 107739     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3710407..3721270
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BST95_RS17430 (BST95_17435) - 3711495..3711986 (-) 492 WP_157114514.1 pilin -
  BST95_RS17435 (BST95_17440) pilB 3712413..3714128 (+) 1716 WP_084200729.1 type IV-A pilus assembly ATPase PilB Machinery gene
  BST95_RS17440 (BST95_17445) pilC 3714159..3715376 (+) 1218 WP_084200730.1 type II secretion system F family protein Machinery gene
  BST95_RS17445 (BST95_17450) pilD 3715407..3716270 (+) 864 WP_084200731.1 prepilin peptidase Machinery gene
  BST95_RS17450 (BST95_17455) coaE 3716267..3716857 (+) 591 WP_084200732.1 dephospho-CoA kinase -
  BST95_RS17455 (BST95_17460) mutT 3716849..3717244 (-) 396 WP_084200733.1 8-oxo-dGTP diphosphatase MutT -
  BST95_RS17460 (BST95_17465) argJ 3717245..3718471 (-) 1227 WP_084200734.1 bifunctional glutamate N-acetyltransferase/amino-acid acetyltransferase ArgJ -
  BST95_RS17465 (BST95_17470) secA 3718481..3721183 (-) 2703 WP_084200735.1 preprotein translocase subunit SecA -

Sequence

Protein


Download         Length: 287 a.a.        Molecular weight: 31240.44 Da        Isoelectric Point: 5.2137

>NTDB_id=215907 BST95_RS17445 WP_084200731.1 3715407..3716270(+) (pilD) [Halioglobus japonicus strain NBRC 107739]
MSEILALHPGLTYTVLLALGLIVGSFLNVVIHRLPIMMESRWRRDCCELLEVEPEKEEPQLTLATPNSHCPACQAPIKPW
QNIPVLSYLLLGGSCANCGVRISVRYPIIELLTGLMTLALAWFFPPSPALLGAMLLTWALIALTMIDIDHQLLPDDITLP
LMWLGLLFNLCGTFVPIEQAVIGAMGGYLSLWSVYWIFKLLTGKEGMGYGDFKLLAALGAWLGWQMLPLIILLSSVVGAV
VGIGLMIARNRGREVPIPFGPYLAAAGWLALIVGDTLNSTLLGVAPQ

Nucleotide


Download         Length: 864 bp        

>NTDB_id=215907 BST95_RS17445 WP_084200731.1 3715407..3716270(+) (pilD) [Halioglobus japonicus strain NBRC 107739]
ATGTCTGAAATCCTCGCCCTGCACCCGGGGCTGACCTACACCGTGCTGCTGGCACTGGGTCTTATTGTCGGCAGTTTTCT
CAATGTGGTAATTCACCGCCTGCCCATCATGATGGAGTCGCGCTGGCGGCGCGACTGCTGTGAGCTGCTCGAGGTTGAGC
CGGAGAAAGAAGAGCCGCAACTCACCCTCGCGACACCCAACTCCCACTGCCCGGCCTGCCAGGCACCGATTAAACCCTGG
CAGAATATTCCTGTGCTTAGCTATCTGTTGCTGGGTGGCAGCTGCGCCAACTGCGGAGTGCGCATCTCAGTTCGCTACCC
CATTATTGAGCTGCTCACTGGCCTGATGACACTGGCATTGGCCTGGTTCTTTCCGCCCTCGCCGGCACTGCTGGGCGCCA
TGCTGCTGACCTGGGCACTGATCGCACTCACCATGATCGACATCGATCATCAGTTACTCCCGGACGACATTACGCTTCCA
CTCATGTGGCTCGGACTGTTGTTTAACCTGTGCGGTACCTTTGTGCCCATTGAGCAGGCCGTCATCGGCGCCATGGGCGG
CTACTTGAGCCTGTGGAGCGTCTACTGGATATTCAAACTGCTCACCGGCAAGGAAGGCATGGGCTATGGCGACTTTAAAC
TCCTGGCCGCCCTCGGGGCCTGGCTGGGCTGGCAGATGCTGCCCCTGATTATCCTGCTATCCTCCGTGGTTGGCGCTGTC
GTCGGCATCGGGCTGATGATTGCCCGCAATCGCGGCCGCGAAGTACCCATCCCCTTTGGCCCCTATCTCGCCGCGGCCGG
ATGGTTGGCGCTGATAGTGGGAGACACGCTTAACAGCACGCTGCTGGGAGTTGCGCCCCAGTGA

Domains

Predicted by InterproScan.

Bacterial Peptidase A24 N-terminal domain (A24_N_bact)

(19-124)

Type IV leader peptidase family (Peptidase_A24)

(134-243)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilD Vibrio campbellii strain DS40M4

53.103

100

0.537
  pilD Vibrio cholerae strain A1552

54.676

96.864

0.53
  pilD Acinetobacter baumannii D1279779

52.941

94.774

0.502
  pilD Acinetobacter nosocomialis M2

52.206

94.774

0.495
  pilD Neisseria gonorrhoeae MS11

50.714

97.561

0.495

Multiple sequence alignment