Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilE   Type   Machinery gene
Locus tag   BWP33_RS11960 Genome accession   NZ_CP019448
Coordinates   2414436..2414957 (+) Length   173 a.a.
NCBI ID   WP_002642764.1    Uniprot ID   V9H5L1
Organism   Simonsiella muelleri ATCC 29453     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2409436..2419957
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BWP33_RS11940 (BWP33_11955) cueR 2410029..2410421 (+) 393 WP_002642760.1 Cu(I)-responsive transcriptional regulator -
  BWP33_RS11945 (BWP33_11960) - 2410805..2412325 (-) 1521 WP_002642761.1 fumarate hydratase -
  BWP33_RS11950 (BWP33_11965) ampD 2412615..2413187 (-) 573 WP_002642762.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  BWP33_RS11955 (BWP33_11970) - 2413363..2414037 (+) 675 WP_002642763.1 YdcF family protein -
  BWP33_RS11960 (BWP33_11975) pilE 2414436..2414957 (+) 522 WP_002642764.1 pilin Machinery gene
  BWP33_RS11965 (BWP33_11980) pilE 2415217..2415738 (+) 522 WP_002642765.1 pilin Machinery gene
  BWP33_RS11970 (BWP33_11985) edd 2415811..2417643 (-) 1833 WP_002642766.1 phosphogluconate dehydratase -
  BWP33_RS11975 (BWP33_11990) - 2417731..2418486 (+) 756 WP_002641156.1 IS5 family transposase -
  BWP33_RS11980 (BWP33_11995) ribF 2418754..2419677 (-) 924 WP_002642767.1 bifunctional riboflavin kinase/FAD synthetase -

Sequence

Protein


Download         Length: 173 a.a.        Molecular weight: 17843.53 Da        Isoelectric Point: 9.5613

>NTDB_id=215876 BWP33_RS11960 WP_002642764.1 2414436..2414957(+) (pilE) [Simonsiella muelleri ATCC 29453]
MKTMQKGFTLIELMIVIAIIGILAAIALPAYQDYTVRSKISEALIASSSAKGMVSEAFQSDSLSGVATAATAYNAKPKSE
KSSKYVDDIVVNAADGAITVTLSKASNVGFPAEVKGKTIVFTPYVKKAKLAEGAQGAIDWACASNANGTATARGLTSPTA
GTLPSKYAPSECR

Nucleotide


Download         Length: 522 bp        

>NTDB_id=215876 BWP33_RS11960 WP_002642764.1 2414436..2414957(+) (pilE) [Simonsiella muelleri ATCC 29453]
ATGAAAACTATGCAAAAAGGTTTCACATTAATCGAGTTGATGATTGTAATCGCTATTATCGGTATTTTGGCTGCTATCGC
ATTGCCTGCTTACCAAGACTACACTGTTCGCTCAAAAATTTCTGAGGCTTTGATTGCTTCTTCTTCTGCTAAAGGCATGG
TATCTGAAGCATTCCAATCTGATAGTTTGTCTGGTGTAGCAACTGCTGCTACTGCTTATAATGCCAAACCTAAGTCTGAA
AAATCTTCTAAATATGTTGATGATATCGTGGTTAATGCAGCTGATGGTGCAATCACTGTAACGTTGTCTAAAGCTAGCAA
CGTAGGTTTCCCAGCTGAAGTTAAAGGTAAAACTATTGTCTTCACTCCATATGTGAAAAAAGCTAAGTTGGCTGAAGGTG
CGCAAGGTGCGATTGACTGGGCTTGTGCTTCTAATGCAAACGGTACTGCAACTGCTCGTGGTTTAACATCACCAACTGCT
GGTACTTTGCCATCTAAATACGCTCCTTCTGAGTGTCGTTAA

Domains

Predicted by InterproScan.

Prokaryotic N-terminal methylation motif (N_methyl)

(4-28)

Pilin (bacterial filament) (Pilin)

(36-173)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB V9H5L1

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilE Neisseria elongata subsp. glycolytica ATCC 29315

56.383

100

0.613
  pilA/pilA1 Eikenella corrodens VA1

44.099

93.064

0.41
  comP Acinetobacter baylyi ADP1

39.773

100

0.405
  pilA2 Legionella pneumophila str. Paris

41.42

97.688

0.405
  pilA2 Legionella pneumophila strain ERS1305867

40.828

97.688

0.399
  pilA/pilAI Pseudomonas stutzeri DSM 10701

39.645

97.688

0.387
  pilA Ralstonia pseudosolanacearum GMI1000

37.64

100

0.387
  pilE Neisseria gonorrhoeae strain FA1090

35

100

0.364

Multiple sequence alignment