Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   PspS35_RS04700 Genome accession   NZ_CP019431
Coordinates   1052150..1053358 (-) Length   402 a.a.
NCBI ID   WP_159932954.1    Uniprot ID   A0A6I6VV38
Organism   Pseudomonas sp. S35     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1047150..1058358
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PspS35_RS04665 (PspS35_04755) - 1048504..1048677 (-) 174 WP_044286631.1 DUF3094 family protein -
  PspS35_RS04670 (PspS35_04760) - 1048733..1049362 (-) 630 WP_010213242.1 DUF1780 domain-containing protein -
  PspS35_RS04675 (PspS35_04765) - 1049492..1050181 (+) 690 WP_159932951.1 energy-coupling factor ABC transporter permease -
  PspS35_RS04680 (PspS35_04770) - 1050258..1050473 (+) 216 WP_003171680.1 hypothetical protein -
  PspS35_RS04685 (PspS35_04775) yacG 1050470..1050670 (-) 201 WP_003188437.1 DNA gyrase inhibitor YacG -
  PspS35_RS04690 (PspS35_04780) coaE 1050667..1051290 (-) 624 WP_159932952.1 dephospho-CoA kinase -
  PspS35_RS04695 (PspS35_04785) pilD 1051287..1052153 (-) 867 WP_159932953.1 A24 family peptidase Machinery gene
  PspS35_RS04700 (PspS35_04790) pilC 1052150..1053358 (-) 1209 WP_159932954.1 type II secretion system F family protein Machinery gene
  PspS35_RS04705 (PspS35_04795) - 1053561..1053974 (+) 414 WP_159937981.1 pilin -
  PspS35_RS04710 (PspS35_04800) - 1054010..1055410 (+) 1401 WP_159932955.1 O-antigen ligase family protein -
  PspS35_RS04720 (PspS35_04810) - 1055695..1056000 (+) 306 WP_159932956.1 DUF6388 family protein -
  PspS35_RS04725 (PspS35_04815) nadC 1056074..1056922 (-) 849 WP_159932957.1 carboxylating nicotinate-nucleotide diphosphorylase -

Sequence

Protein


Download         Length: 402 a.a.        Molecular weight: 43754.09 Da        Isoelectric Point: 9.9782

>NTDB_id=215723 PspS35_RS04700 WP_159932954.1 1052150..1053358(-) (pilC) [Pseudomonas sp. S35]
MTNASTLYAWEGINRKGRRVSGQITGYNPALVKAQLREQGISLARVRKASPVWQRLAPPVKTADITLLTRQLATLLKAGI
PLLQAFDIISEGFDNRSVRELVQQLKQAIAAGTNLADALRKQPRYFDDLYCNLVAVGEQAGALETLLERVATHREKSEQL
TARIKKAMAYPIAVLVVASLVTSVLLIHVVPQFQALFAGVNGKLPDFTLQVIALSEFMQQAWWMLAIGIATGAVGLRQAY
RTLPGFRLWLDTGLLKAPLIGKLLTKSAVARYARTLSTTFAAGVPLVQALDSVAGAVGNDPFKQAIERMRHDVSTGMQLN
QSMANNRLFPGMAIQMTAIGEESGTLDHMLEKVASHYETDVDNHVDTLTNLMEPLIMVVLGGIVGALVIALYLPVFQLGS
AF

Nucleotide


Download         Length: 1209 bp        

>NTDB_id=215723 PspS35_RS04700 WP_159932954.1 1052150..1053358(-) (pilC) [Pseudomonas sp. S35]
ATGACGAACGCTTCGACGCTCTACGCCTGGGAAGGCATCAACCGCAAAGGACGCAGGGTGTCCGGACAAATCACGGGGTA
CAACCCTGCCCTGGTCAAGGCACAGTTGCGCGAACAAGGGATCAGCCTCGCTCGCGTGCGCAAGGCATCGCCGGTGTGGC
AACGCCTTGCCCCACCGGTCAAGACGGCCGACATCACCCTGCTCACTCGTCAACTGGCCACCCTGCTGAAGGCTGGGATT
CCCCTTCTGCAAGCCTTCGACATCATCAGCGAGGGCTTCGACAACCGATCGGTACGTGAACTGGTGCAGCAACTTAAACA
AGCAATCGCCGCCGGTACCAACCTGGCCGATGCCTTGCGTAAACAGCCGCGCTATTTCGACGACCTGTACTGCAACCTGG
TGGCCGTCGGCGAACAAGCCGGGGCACTTGAAACCCTGCTGGAGCGAGTAGCAACCCACCGCGAAAAAAGTGAACAGCTC
ACGGCCAGGATAAAAAAAGCAATGGCCTACCCTATCGCAGTGCTGGTAGTGGCCAGCCTGGTCACCAGTGTGCTTTTGAT
CCACGTAGTGCCGCAGTTCCAAGCCCTGTTTGCGGGAGTCAACGGCAAATTGCCCGACTTCACCTTGCAGGTGATCGCGC
TCTCGGAGTTCATGCAGCAAGCATGGTGGATGCTGGCGATAGGCATAGCGACCGGTGCCGTGGGGCTGCGCCAGGCCTAT
CGAACCTTGCCGGGTTTTCGCTTGTGGCTCGACACCGGTTTGTTGAAAGCGCCCCTGATAGGCAAACTGCTGACAAAATC
CGCCGTGGCCCGCTACGCCCGCACGCTTTCCACCACCTTCGCTGCGGGCGTTCCGCTGGTGCAGGCACTGGATTCGGTAG
CCGGCGCCGTGGGTAACGACCCGTTCAAACAGGCAATCGAGCGTATGCGTCACGATGTATCCACTGGCATGCAGCTGAAT
CAATCCATGGCCAACAACCGCCTTTTTCCCGGCATGGCTATCCAGATGACGGCCATCGGCGAAGAGTCAGGCACGCTGGA
CCACATGCTGGAAAAGGTCGCCAGCCACTATGAGACCGATGTGGACAACCACGTCGACACCCTCACCAACCTGATGGAAC
CGTTGATTATGGTGGTGCTGGGGGGCATCGTCGGAGCGCTGGTCATTGCCCTGTATCTGCCGGTCTTCCAACTGGGGAGC
GCATTTTGA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(273-394)

Type II secretion system (T2SS), protein F (T2SSF)

(69-191)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A6I6VV38

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

58.838

98.507

0.58
  pilC Acinetobacter baumannii D1279779

50.633

98.259

0.498
  pilC Acinetobacter baylyi ADP1

49.875

99.751

0.498
  pilC Legionella pneumophila strain ERS1305867

49.495

98.507

0.488
  pilC Vibrio cholerae strain A1552

42.386

98.01

0.415
  pilC Vibrio campbellii strain DS40M4

38.832

98.01

0.381
  pilG Neisseria meningitidis 44/76-A

38.642

95.274

0.368
  pilG Neisseria gonorrhoeae MS11

38.32

94.776

0.363

Multiple sequence alignment