Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   PspR76_RS02300 Genome accession   NZ_CP019428
Coordinates   523725..525050 (+) Length   441 a.a.
NCBI ID   WP_159961297.1    Uniprot ID   -
Organism   Pseudomonas sp. R76     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 518725..530050
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PspR76_RS02280 (PspR76_02255) - 518791..521256 (-) 2466 WP_159953781.1 penicillin-binding protein 1A -
  PspR76_RS02285 (PspR76_02260) pilM 521483..522139 (+) 657 WP_159961295.1 type IV pilus assembly protein PilM -
  PspR76_RS02290 (PspR76_02265) - 522136..522687 (+) 552 WP_159953782.1 PilN domain-containing protein -
  PspR76_RS02295 (PspR76_02270) - 522684..523655 (+) 972 WP_159953783.1 pilus assembly protein PilP -
  PspR76_RS02300 (PspR76_02275) pilQ 523725..525050 (+) 1326 WP_159961297.1 type IV pilus secretin PilQ family protein Machinery gene
  PspR76_RS02305 (PspR76_02280) aroK 525055..525573 (+) 519 WP_017138567.1 shikimate kinase AroK -
  PspR76_RS02310 (PspR76_02285) aroB 525698..526798 (+) 1101 WP_159953784.1 3-dehydroquinate synthase -
  PspR76_RS02315 (PspR76_02290) - 526809..528371 (+) 1563 WP_159953785.1 AAA family ATPase -

Sequence

Protein


Download         Length: 441 a.a.        Molecular weight: 47924.03 Da        Isoelectric Point: 5.0177

>NTDB_id=215683 PspR76_RS02300 WP_159961297.1 523725..525050(+) (pilQ) [Pseudomonas sp. R76]
MALAVPNRVDLIQLPPPGRAVPSTYSGDKLNLNFQNIQLRAVLQQIADVAGLNLVTSDEVQGSITLRLKDVPWDQALDLV
LQTKGLDKRVKAGVLLVAPAEELAARELLALESNKQMADLAPLRRELLQVNYAKAADLAKLFQSVTTLDGLPDERGSVAV
DDRTNNIIAYQTGERLEELRRIVAQLDIPVRQVMIEARIVEANVDYDKSLGVRWGGRLNRGNWGAGGIGKPLAEGVEPPA
SPSSSPFVDLGSLTGTAGLGIAYITDNLLLDLELTAMEKTGNGEIVSQPKVVTSDKETARILKGTEIPYQESTGRGATSV
SFKEASLSLEVTPQITPDGWVIMEVKVTKDEPDYLNKLNDVPPIKKNEVNAKVLVKDGETIVIGGVFSNTQSKVVDKVPF
LGDVPYLGRLFRRDVVAQRKSELLVFLTPRIMNNQAIAVSR

Nucleotide


Download         Length: 1326 bp        

>NTDB_id=215683 PspR76_RS02300 WP_159961297.1 523725..525050(+) (pilQ) [Pseudomonas sp. R76]
ATGGCCCTTGCTGTGCCCAACCGTGTAGATCTGATCCAACTGCCGCCGCCTGGCCGCGCGGTGCCAAGCACCTATAGCGG
TGACAAGCTGAACCTCAACTTCCAGAACATCCAGTTGCGTGCGGTATTGCAGCAGATAGCGGATGTGGCCGGGCTCAACC
TGGTCACCAGCGACGAGGTGCAAGGCTCGATCACCCTGAGACTCAAGGATGTGCCCTGGGATCAGGCGCTGGACCTGGTG
TTGCAAACCAAAGGGTTGGACAAGCGCGTGAAAGCCGGCGTTTTGCTGGTGGCGCCCGCCGAAGAATTGGCCGCCCGTGA
GTTGCTGGCGTTGGAGTCGAACAAGCAGATGGCCGACTTGGCGCCATTGCGCCGGGAGCTGTTGCAGGTCAATTACGCCA
AGGCCGCGGACCTGGCCAAGCTGTTTCAGTCGGTGACGACCCTGGATGGCCTCCCCGACGAGCGCGGTTCAGTGGCGGTG
GATGACCGCACCAACAACATCATTGCCTACCAGACCGGCGAGCGGCTCGAAGAGCTGCGACGGATCGTGGCGCAGCTGGA
TATTCCAGTACGCCAAGTGATGATTGAGGCGCGCATCGTCGAAGCCAATGTCGATTACGACAAAAGCCTGGGCGTGCGCT
GGGGCGGGCGCCTTAATCGTGGCAATTGGGGTGCGGGCGGCATCGGCAAGCCGTTGGCCGAGGGCGTTGAACCACCGGCG
AGCCCGTCCAGCTCGCCGTTTGTTGACTTGGGCTCACTCACTGGCACTGCGGGTTTGGGCATTGCCTATATCACCGACAA
CCTGTTGTTGGACCTGGAGCTGACGGCCATGGAGAAAACCGGCAACGGCGAAATCGTCTCGCAGCCCAAGGTCGTCACCT
CCGACAAGGAGACCGCGCGCATCCTTAAAGGCACCGAGATTCCCTATCAGGAATCCACCGGCAGAGGTGCCACGTCGGTG
TCCTTCAAGGAAGCCTCGCTGTCGCTGGAGGTGACCCCGCAAATCACGCCAGATGGCTGGGTGATCATGGAGGTCAAGGT
CACCAAGGATGAGCCCGACTACCTGAACAAACTCAACGACGTGCCGCCGATCAAGAAAAACGAAGTCAACGCCAAGGTAC
TGGTCAAGGACGGCGAGACCATCGTCATCGGCGGGGTTTTCTCCAATACCCAAAGCAAAGTGGTAGATAAAGTGCCATTT
TTGGGCGATGTGCCGTATCTTGGCCGCCTTTTCCGGCGCGATGTTGTGGCGCAAAGAAAATCCGAGCTGCTGGTATTCCT
GACTCCGCGTATTATGAATAACCAGGCGATTGCTGTGAGTCGTTGA

Domains

Predicted by InterproScan.

Secretin and TonB N terminus short domain (STN)

(54-99)

Bacterial type II/III secretion system short domain (Secretin_N)

(127-192)

Bacterial type II and III secretion system protein (Secretin)

(276-432)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

71.101

98.866

0.703
  pilQ Acinetobacter baumannii D1279779

40.365

100

0.451
  pilQ Acinetobacter baumannii strain A118

40.365

100

0.451
  comQ Acinetobacter baylyi ADP1

42.444

100

0.433
  pilQ Vibrio campbellii strain DS40M4

44.208

95.918

0.424
  pilQ Legionella pneumophila strain ERS1305867

44.048

95.238

0.42
  pilQ Legionella pneumophila strain Lp02

43.839

95.692

0.42
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

43.165

94.558

0.408
  pilQ Vibrio cholerae strain A1552

43.165

94.558

0.408

Multiple sequence alignment