Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   BST96_RS02255 Genome accession   NZ_CP019343
Coordinates   490549..492258 (+) Length   569 a.a.
NCBI ID   WP_085757125.1    Uniprot ID   A0A1X9N733
Organism   Oceanicoccus sagamiensis strain NBRC 107125     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 485549..497258
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BST96_RS02235 (BST96_02235) - 485680..486714 (-) 1035 WP_085757121.1 DUF354 domain-containing protein -
  BST96_RS02240 (BST96_02240) - 486718..487581 (-) 864 WP_085757122.1 glycosyltransferase family 2 protein -
  BST96_RS02245 (BST96_02245) - 487586..489547 (-) 1962 WP_085757123.1 hypothetical protein -
  BST96_RS02250 (BST96_02250) - 489886..490326 (+) 441 WP_085757124.1 pilin -
  BST96_RS02255 (BST96_02255) pilB 490549..492258 (+) 1710 WP_085757125.1 type IV-A pilus assembly ATPase PilB Machinery gene
  BST96_RS02260 (BST96_02260) pilC 492262..493479 (+) 1218 WP_085757126.1 type II secretion system F family protein Machinery gene
  BST96_RS02265 (BST96_02265) pilD 493492..494376 (+) 885 WP_085757127.1 prepilin peptidase Machinery gene
  BST96_RS02270 (BST96_02270) coaE 494438..495049 (+) 612 WP_085757128.1 dephospho-CoA kinase -
  BST96_RS02275 (BST96_02275) - 495039..495239 (+) 201 WP_085757129.1 DNA gyrase inhibitor YacG -
  BST96_RS02280 (BST96_02280) - 495298..495747 (-) 450 WP_085757130.1 hypothetical protein -
  BST96_RS02285 (BST96_02285) - 496015..496962 (+) 948 WP_085757131.1 pentapeptide repeat-containing protein -

Sequence

Protein


Download         Length: 569 a.a.        Molecular weight: 62199.06 Da        Isoelectric Point: 4.5588

>NTDB_id=214947 BST96_RS02255 WP_085757125.1 490549..492258(+) (pilB) [Oceanicoccus sagamiensis strain NBRC 107125]
MNAIPLSGLARRLVKDALLSEEAAQEAFQSASTEKMPFVSYLISEGLCDSGAIAAAASDEFGAPLFDLVSFNMEMAPKEL
VDIKLIQQHHTLPLYRRGNRLFIAVSDPTNLRALDEIKFHTGINTDAVLVDEALLSEAIAKYVEANDEPLGGFDDLEDGL
DDLDIEAVDEDGGGEAEQGGDIDETPIVRFVNKVLIDAIKSGASDIHFEPYEKSYRVRFRTDGILTEVTRPPMTLSGRLA
ARLKVMSQMDISERRLPQDGRIKMKLSKKRAIDFRVNTLPTLFGEKVVLRILDPSSAQMGIDALGYEPEQKEMYMHALKQ
PQGMILVTGPTGSGKTVSLYTGLNILNEPEVNISTAEDPVEINLEGINQCHVNPKVGLTFAEALRSFLRQDPDVIMVGEI
RDLETAEISIKAAQTGHMVMSTLHTNSAAETITRLMNMGVPAFNVATSVSLIIAQRLARRLCSECKEPADIPKETLLAEG
FTEADLEEATLMKPVGCSKCSSGSKGRVGIYEVVRITDEISRIIMEGGNSIEIGDKARAEGFNDLRRSALMKAAQGLISL
EEVNRVTKD

Nucleotide


Download         Length: 1710 bp        

>NTDB_id=214947 BST96_RS02255 WP_085757125.1 490549..492258(+) (pilB) [Oceanicoccus sagamiensis strain NBRC 107125]
ATGAATGCAATACCCTTAAGCGGCTTAGCCCGGCGTCTGGTTAAAGATGCACTGTTAAGTGAAGAGGCAGCACAAGAAGC
CTTTCAAAGTGCCAGCACTGAGAAAATGCCCTTTGTCTCTTACCTGATCTCTGAGGGTCTGTGTGACAGTGGAGCTATTG
CTGCAGCGGCATCTGACGAGTTTGGCGCTCCCTTGTTTGACCTCGTCTCCTTCAATATGGAAATGGCACCCAAAGAACTG
GTCGATATTAAGCTAATCCAGCAGCACCATACACTGCCCTTATATCGACGCGGCAACAGGCTCTTTATTGCTGTTTCAGA
CCCTACCAATCTTAGAGCACTTGATGAGATCAAATTTCATACAGGCATCAATACTGATGCGGTATTGGTTGATGAAGCAT
TACTAAGCGAAGCGATTGCCAAATATGTCGAGGCCAATGATGAACCTCTTGGAGGGTTTGACGACCTTGAAGATGGTCTC
GATGACCTGGATATTGAAGCCGTTGATGAAGATGGCGGCGGTGAAGCCGAACAAGGTGGCGACATTGATGAAACACCAAT
CGTCCGCTTTGTTAACAAGGTTCTTATTGATGCCATTAAATCCGGCGCATCGGATATCCACTTTGAACCCTATGAGAAAA
GTTACCGGGTACGCTTTCGTACCGATGGTATCCTGACCGAAGTGACCCGACCCCCCATGACTCTGTCAGGTCGCTTGGCT
GCACGTTTGAAAGTTATGTCGCAAATGGATATTTCAGAGCGACGCCTGCCACAGGATGGCCGCATCAAGATGAAGCTATC
TAAAAAGCGCGCTATCGATTTCCGGGTCAACACCCTGCCCACCTTATTTGGTGAAAAGGTGGTGCTGCGTATACTTGACC
CCAGCAGTGCCCAGATGGGTATTGATGCACTCGGTTATGAACCCGAACAAAAAGAAATGTATATGCACGCCCTGAAACAA
CCCCAGGGTATGATACTGGTGACCGGGCCTACCGGTAGTGGTAAAACCGTATCTCTGTATACCGGCCTCAATATCTTGAA
CGAACCCGAGGTCAATATCTCAACGGCGGAAGACCCGGTTGAAATTAACCTGGAAGGTATTAACCAATGCCATGTTAATC
CTAAAGTCGGCCTGACCTTTGCCGAGGCTCTGCGCTCTTTTCTACGTCAGGATCCCGATGTCATTATGGTGGGTGAGATT
CGTGACCTTGAAACCGCCGAGATTTCTATTAAGGCGGCACAAACCGGCCATATGGTAATGTCCACACTGCACACTAACAG
CGCAGCAGAGACCATTACTCGCTTGATGAATATGGGGGTCCCGGCATTTAATGTGGCCACCTCAGTTAGCTTAATTATCG
CCCAGCGATTAGCGCGCCGCCTATGCAGCGAGTGTAAAGAACCCGCGGATATACCCAAAGAAACGCTACTGGCGGAAGGC
TTTACTGAAGCCGATTTGGAGGAAGCCACACTGATGAAACCCGTTGGCTGCTCCAAATGCAGCAGCGGCAGCAAAGGCCG
AGTGGGAATATATGAAGTAGTTCGCATCACCGATGAAATCTCCCGTATTATTATGGAAGGTGGTAATTCGATAGAAATTG
GCGACAAAGCCCGTGCAGAAGGTTTCAACGACCTCCGCAGATCGGCATTAATGAAAGCAGCCCAAGGGTTAATTAGCCTG
GAAGAGGTTAATCGGGTAACGAAGGATTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(42-144)

Type II/IV secretion system protein (T2SSE)

(193-460)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1X9N733

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

61.552

99.649

0.613
  pilB Acinetobacter baylyi ADP1

59.474

100

0.596
  pilB Legionella pneumophila strain ERS1305867

54.497

99.649

0.543
  pilB Vibrio parahaemolyticus RIMD 2210633

50.262

100

0.506
  pilB Vibrio campbellii strain DS40M4

49.129

100

0.496
  pilF Neisseria gonorrhoeae MS11

48.768

99.824

0.487
  pilB Vibrio cholerae strain A1552

53.529

89.631

0.48
  pilF Thermus thermophilus HB27

37.343

97.891

0.366
  pilB/pilB1 Synechocystis sp. PCC 6803

41.2

87.873

0.362

Multiple sequence alignment