Detailed information NTG0214949

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: BST96_RS02265
• Genome accession: NZ_CP019343
• Coordinates: 493492..494376 (+)
• Length: 294 a.a.
• NCBI ID: WP_085757127.1
• Uniprot ID: A0A1X9NG92
• Organism: Oceanicoccus sagamiensis strain NBRC 107125
• Function: assembly of type IV pilus (predicted from homology) DNA binding and uptake

Genomic Context

Location: 488492..499376

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
BST96_RS02250 (BST96_02250)	-	489886..490326 (+)	441	WP_085757124.1	pilin	-
BST96_RS02255 (BST96_02255)	pilB	490549..492258 (+)	1710	WP_085757125.1	type IV-A pilus assembly ATPase PilB	Machinery gene
BST96_RS02260 (BST96_02260)	pilC	492262..493479 (+)	1218	WP_085757126.1	type II secretion system F family protein	Machinery gene
BST96_RS02265 (BST96_02265)	pilD	493492..494376 (+)	885	WP_085757127.1	prepilin peptidase	Machinery gene
BST96_RS02270 (BST96_02270)	coaE	494438..495049 (+)	612	WP_085757128.1	dephospho-CoA kinase	-
BST96_RS02275 (BST96_02275)	-	495039..495239 (+)	201	WP_085757129.1	DNA gyrase inhibitor YacG	-
BST96_RS02280 (BST96_02280)	-	495298..495747 (-)	450	WP_085757130.1	hypothetical protein	-
BST96_RS02285 (BST96_02285)	-	496015..496962 (+)	948	WP_085757131.1	pentapeptide repeat-containing protein	-
BST96_RS02290 (BST96_02290)	-	497078..497683 (+)	606	WP_157117817.1	TetR/AcrR family transcriptional regulator	-
BST96_RS02295 (BST96_02295)	-	497699..498811 (-)	1113	WP_338043312.1	sensor domain-containing diguanylate cyclase	-

Sequence

Protein

• Length: 294 a.a.
• Molecular weight: 32459.77 Da
• Isoelectric Point: 8.2048

>NTDB_id=214949 BST96_RS02265 WP_085757127.1 493492..494376(+) (pilD) [Oceanicoccus sagamiensis strain NBRC 107125]
MTLLEFLQASPALTIGLTVIIGLLVGSFLNVVIYRLPLMMEKEWKQECRIILELDKDQPPAKTEAKLTLSYPNSHCPSCN
NPIKPWQNIPVISYLLLKGQCAHCKTAISIRYPIIEAVTGIMSGILAWYLGASPQLLMALFFTWCLISLTMIDADTQLLP
DQITLPLLWAGLLVNSFGLFVPLYDAVWGAIGGYLSLWSVYWLFKILTGKEGMGYGDFKLLAALGAWMGWQYLLVIILLS
SLVGAVVGSILLAVNKKGRNTTIPFGPYLAAAGWIAFLWGDQIISTYLRVSGIK

Nucleotide

• Length: 885 bp

>NTDB_id=214949 BST96_RS02265 WP_085757127.1 493492..494376(+) (pilD) [Oceanicoccus sagamiensis strain NBRC 107125]
ATGACATTATTAGAGTTTCTACAGGCCTCGCCTGCGTTAACGATTGGACTGACGGTTATTATCGGCCTGCTGGTCGGCAG
TTTTCTCAATGTGGTGATCTATCGCCTGCCCTTGATGATGGAAAAAGAGTGGAAGCAGGAGTGCCGTATCATTCTGGAAC
TGGATAAAGACCAGCCACCGGCCAAAACCGAAGCAAAACTGACTTTATCCTATCCAAACTCCCACTGCCCAAGCTGCAAC
AACCCCATCAAGCCCTGGCAAAATATCCCGGTGATTAGCTACCTGCTACTCAAAGGCCAGTGTGCTCATTGTAAAACGGC
AATATCGATTCGCTACCCCATTATTGAAGCCGTGACCGGCATTATGTCTGGCATCCTGGCCTGGTATTTAGGCGCCTCGC
CACAGCTATTAATGGCCCTGTTCTTTACCTGGTGCTTAATCAGTTTAACGATGATTGATGCCGACACCCAATTACTGCCT
GACCAAATCACCCTCCCCCTCCTCTGGGCTGGTTTATTAGTCAATAGCTTTGGGTTATTTGTCCCACTCTATGATGCCGT
ATGGGGCGCTATAGGCGGCTATCTAAGTTTATGGTCAGTTTATTGGTTGTTTAAAATCCTCACCGGCAAGGAAGGTATGG
GCTACGGCGATTTTAAATTGCTGGCAGCTCTTGGGGCCTGGATGGGCTGGCAATATCTACTGGTTATTATCTTACTGTCA
TCACTGGTTGGTGCCGTCGTCGGTAGTATTCTATTGGCAGTCAATAAAAAAGGCCGCAATACCACCATTCCTTTTGGGCC
TTATCTGGCAGCCGCCGGCTGGATCGCATTCCTTTGGGGCGACCAGATTATTAGCACCTACCTCCGTGTTAGCGGCATCA
AATAA

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (20-129)

• Type IV leader peptidase family (Peptidase_A24) (141-248)

3D structure

• AlphaFold DB: A0A1X9NG92

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio cholerae strain A1552	56.294	97.279	0.548
pilD	Vibrio campbellii strain DS40M4	54.196	97.279	0.527
pilD	Acinetobacter nosocomialis M2	54.77	96.259	0.527
pilD	Acinetobacter baumannii D1279779	54.417	96.259	0.524
pilD	Neisseria gonorrhoeae MS11	52.399	92.177	0.483