Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   FORC53_RS02915 Genome accession   NZ_CP019290
Coordinates   582677..584365 (-) Length   562 a.a.
NCBI ID   WP_118894060.1    Uniprot ID   A0AAN1PLS7
Organism   Vibrio vulnificus strain FORC_053     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 577677..589365
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FORC53_RS02885 (FORC53_0506) rplS 578206..578559 (+) 354 WP_011079544.1 50S ribosomal protein L19 -
  FORC53_RS02890 yacG 578800..578994 (-) 195 WP_118893299.1 DNA gyrase inhibitor YacG -
  FORC53_RS02895 (FORC53_0507) zapD 579057..579797 (-) 741 WP_011079546.1 cell division protein ZapD -
  FORC53_RS02900 (FORC53_0508) coaE 579830..580438 (-) 609 WP_017789873.1 dephospho-CoA kinase -
  FORC53_RS02905 (FORC53_0509) pilD 580440..581309 (-) 870 WP_118893301.1 prepilin peptidase Machinery gene
  FORC53_RS02910 (FORC53_0510) pilC 581403..582629 (-) 1227 WP_045618629.1 type II secretion system F family protein Machinery gene
  FORC53_RS02915 (FORC53_0511) pilB 582677..584365 (-) 1689 WP_118894060.1 type IV-A pilus assembly ATPase PilB Machinery gene
  FORC53_RS02920 (FORC53_0512) - 584381..584821 (-) 441 WP_118893302.1 pilin -
  FORC53_RS02925 (FORC53_0513) nadC 585044..585931 (-) 888 WP_065090099.1 carboxylating nicotinate-nucleotide diphosphorylase -
  FORC53_RS02930 (FORC53_0514) ampD 586024..586596 (+) 573 WP_072608922.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  FORC53_RS02935 (FORC53_0515) pdhR 586985..587752 (+) 768 WP_011079554.1 pyruvate dehydrogenase complex transcriptional repressor PdhR -

Sequence

Protein


Download         Length: 562 a.a.        Molecular weight: 62425.48 Da        Isoelectric Point: 5.6863

>NTDB_id=214590 FORC53_RS02915 WP_118894060.1 582677..584365(-) (pilB) [Vibrio vulnificus strain FORC_053]
MQSNLVTILRQANQLGLTQEQACRETIQASGVTAPEALLQLGFFQPDELTEKLSAIFGLPRTDLNQYDYQELCNQLGLRE
LITRHQALPLQKSNAGLLLAIADPTNIQAEDDFRFATGVQVEIVLADCQELQAAIRRLYGKSIKQSQSSGLKEIHQDELA
NLVEVSDDEVESIEDLSQDESPVSRFINQILLDAVRKGASDIHFEPYENQYRVRLRCDGILVEVQQPPSHLSRRLSARIK
ILSKLDIAERRLPQDGRIKLRLNQETAIDIRVSTLPTLFGEKIVLRLLDSSSSSLDIDKLGYNPQQKQLYLDALRKPQGM
ILMTGPTGSGKTVSLYTGLSLLNRPEVNISTAEDPVEINLPGINQVQVKPKIGFGFAEALRSFLRQDPDIVMVGEIRDLE
TAEIAVKAAQTGHLVLSTLHTNSAAETIVRLSNMGIASFNLASSLSLIIAQRLARRLCSHCKQPHPYNATFELLGLKADD
LIYQADKQGCHHCTHGYSGRVGIYEVMPFDEQLAEAIVKGASVQSLEMLARQKGMMTLKDSGLEKLKQGITSLEELQRVL
YL

Nucleotide


Download         Length: 1689 bp        

>NTDB_id=214590 FORC53_RS02915 WP_118894060.1 582677..584365(-) (pilB) [Vibrio vulnificus strain FORC_053]
ATGCAATCCAACCTTGTCACCATTCTGCGTCAGGCCAATCAACTTGGCCTAACACAAGAACAAGCGTGTCGAGAGACAAT
ACAAGCTTCTGGTGTGACAGCACCGGAAGCTTTGTTGCAACTGGGATTTTTCCAGCCTGATGAACTAACCGAAAAGCTAA
GCGCGATTTTTGGCTTACCACGCACTGACCTCAACCAATATGACTACCAAGAGCTGTGTAATCAACTTGGGTTACGTGAG
CTAATCACCCGTCACCAAGCATTGCCACTACAAAAAAGCAACGCTGGTTTGCTACTTGCTATTGCCGATCCCACCAATAT
TCAAGCTGAAGATGATTTTCGTTTTGCCACAGGCGTGCAGGTGGAGATTGTTTTAGCTGATTGCCAAGAGCTGCAAGCGG
CGATTCGTAGACTCTATGGAAAGAGCATCAAACAGAGCCAAAGCTCTGGTTTAAAAGAGATTCACCAAGATGAGCTGGCG
AATTTGGTCGAGGTTTCAGATGACGAAGTCGAGTCTATTGAAGACTTAAGCCAAGATGAATCCCCCGTGAGCCGCTTTAT
CAACCAAATCCTGCTTGATGCGGTACGTAAAGGCGCGTCCGATATTCATTTTGAACCGTACGAAAACCAATACCGCGTGC
GATTGCGTTGCGATGGTATTTTGGTGGAAGTGCAACAGCCCCCAAGCCATTTAAGCCGCCGTTTATCCGCGAGGATCAAG
ATCCTCTCCAAGTTGGATATTGCGGAAAGGCGTCTGCCACAAGATGGACGGATTAAGCTGCGCCTCAATCAAGAAACCGC
GATTGATATACGCGTTTCCACCTTACCCACACTGTTTGGTGAAAAAATTGTGTTGCGACTTCTAGACAGCTCGAGCAGCA
GCTTGGATATTGATAAGTTAGGCTACAACCCGCAACAAAAGCAGCTCTACCTCGATGCCCTGCGCAAACCGCAAGGGATG
ATTTTGATGACAGGCCCAACAGGAAGCGGTAAAACCGTTTCTCTGTACACAGGCTTGAGTTTGCTTAACCGGCCAGAAGT
GAATATCTCCACCGCGGAAGACCCGGTCGAAATTAACCTCCCAGGTATCAACCAAGTGCAAGTGAAACCCAAAATTGGTT
TTGGTTTTGCAGAAGCATTGCGTTCTTTTCTCCGTCAAGACCCGGATATCGTCATGGTGGGGGAAATTCGAGATTTGGAA
ACCGCCGAGATCGCCGTCAAAGCAGCCCAAACCGGTCACCTTGTACTATCCACCTTACATACCAACTCGGCTGCTGAAAC
CATTGTTCGTCTATCTAACATGGGGATCGCCAGCTTTAACTTGGCTTCATCCTTGAGTTTGATCATTGCTCAGCGTCTGG
CTCGTCGGTTATGCAGCCATTGCAAGCAACCTCATCCATACAACGCAACATTTGAGTTACTCGGCCTAAAAGCTGACGAC
TTGATTTATCAAGCTGATAAACAGGGTTGCCATCATTGTACTCATGGTTATTCCGGTCGTGTCGGTATATACGAAGTCAT
GCCTTTTGATGAGCAGCTTGCAGAGGCGATCGTGAAAGGCGCCTCGGTACAAAGCTTGGAGATGCTCGCTCGGCAAAAAG
GCATGATGACACTCAAAGATTCCGGCCTTGAAAAACTCAAACAGGGCATCACCAGTTTAGAAGAGCTGCAACGCGTCCTC
TATTTGTAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(38-141)

Type II/IV secretion system protein (T2SSE)

(189-456)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio campbellii strain DS40M4

75.979

100

0.76
  pilB Vibrio parahaemolyticus RIMD 2210633

75.801

100

0.758
  pilB Vibrio cholerae strain A1552

74.067

100

0.742
  pilB Legionella pneumophila strain ERS1305867

52.827

91.281

0.482
  pilB Acinetobacter baumannii D1279779

51.533

92.883

0.479
  pilB Acinetobacter baylyi ADP1

50.383

92.883

0.468
  pilF Neisseria gonorrhoeae MS11

45.133

100

0.454
  pilF Thermus thermophilus HB27

40.966

99.466

0.407
  pilB/pilB1 Synechocystis sp. PCC 6803

37.063

100

0.377
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.491

94.306

0.363

Multiple sequence alignment