Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   FORC53_RS01650 Genome accession   NZ_CP019290
Coordinates   333928..335712 (+) Length   594 a.a.
NCBI ID   WP_011079356.1    Uniprot ID   A0A3Q0L3M5
Organism   Vibrio vulnificus strain FORC_053     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 328928..340712
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FORC53_RS01630 (FORC53_0292) pilM 331242..332249 (+) 1008 WP_017419656.1 type IV pilus assembly protein PilM Machinery gene
  FORC53_RS01635 (FORC53_0293) pilN 332239..332829 (+) 591 WP_011079353.1 PilN domain-containing protein Machinery gene
  FORC53_RS01640 (FORC53_0294) pilO 332822..333412 (+) 591 WP_011079354.1 type 4a pilus biogenesis protein PilO Machinery gene
  FORC53_RS01645 (FORC53_0295) pilP 333402..333914 (+) 513 WP_011079355.1 pilus assembly protein PilP Machinery gene
  FORC53_RS01650 (FORC53_0296) pilQ 333928..335712 (+) 1785 WP_011079356.1 type IV pilus secretin PilQ Machinery gene
  FORC53_RS01655 (FORC53_0297) aroK 335914..336432 (+) 519 WP_011079357.1 shikimate kinase AroK -
  FORC53_RS01660 (FORC53_0298) aroB 336465..337556 (+) 1092 WP_011079358.1 3-dehydroquinate synthase -
  FORC53_RS01665 (FORC53_0299) - 337607..339103 (+) 1497 WP_118893223.1 AAA family ATPase -
  FORC53_RS01670 (FORC53_0300) - 339183..340010 (+) 828 WP_080536747.1 Dam family site-specific DNA-(adenine-N6)-methyltransferase -

Sequence

Protein


Download         Length: 594 a.a.        Molecular weight: 64520.62 Da        Isoelectric Point: 4.5316

>NTDB_id=214578 FORC53_RS01650 WP_011079356.1 333928..335712(+) (pilQ) [Vibrio vulnificus strain FORC_053]
MICELMMKNKIQLFPGMLSQFVCLLSVCIFTTFANAQSEAENALKSIDFRTNAAKDAIIIVELATPATVVDLKPVAEGLS
IELINTSVKDEQLYLLDVKDFSTVVESIEVFREVSKTRLVANVSGEFRHDYRLSGRYLEVTISRIKSEQEAREQSVLEKE
GKLISINFQDIPVRNVLQLIADYNGFNLVVSDSVTGNVTLRLDGVPWQQVLDIILQVKGLDKRVEGNVVLIAPQDELDLR
EKQQLEKERLAEELGSLKSEIIKVNFAKASDIAEMINGKESVSMLSERGSVAIDERTNSLLLRELPENIEVIREIINSLD
IPVKQVQIEARIVTVNEGNLDELGVRWGFTSINGSTTVGGSIENNLATIGLYNAGSGSGSGDSGSGGSGSEGGSASIDDF
LNVNLAATSANASSIAFQVAKLGSDLLLDLELSALQRESKAEIISSPRLITTNKKPAYIEQGTEIPYLESSSSGATTVSF
KKAVLSLKVTPQITPDNRLVLDLSVTQDRPGEIVKTGTGEAVAIDTQRIGTQVLVNNGETVVLGGIFQHSITNTTDKVPL
LGDLPLLGALFRRSYENVGKRELLIFVTPKVILQ

Nucleotide


Download         Length: 1785 bp        

>NTDB_id=214578 FORC53_RS01650 WP_011079356.1 333928..335712(+) (pilQ) [Vibrio vulnificus strain FORC_053]
GTGATTTGTGAGTTGATGATGAAAAACAAAATCCAATTGTTTCCGGGAATGCTTTCCCAATTCGTCTGCCTGTTGTCTGT
TTGCATCTTTACCACCTTTGCAAACGCACAATCTGAAGCAGAAAATGCGCTCAAGAGCATCGATTTCAGAACCAACGCAG
CCAAAGATGCGATTATCATCGTTGAATTGGCAACGCCTGCAACGGTGGTGGATTTGAAACCGGTAGCGGAAGGATTGAGC
ATCGAGTTGATCAATACCTCGGTCAAAGATGAGCAGCTCTATCTGCTCGATGTCAAAGATTTCTCGACCGTGGTTGAAAG
TATCGAAGTATTCAGAGAAGTGTCCAAAACCCGTCTTGTTGCGAATGTTAGTGGTGAATTTCGTCACGACTATCGTCTGT
CTGGACGCTATCTCGAGGTGACAATTTCTCGTATCAAATCGGAACAAGAAGCCCGTGAACAAAGTGTGTTGGAAAAAGAA
GGCAAGTTGATTTCTATCAACTTCCAAGATATTCCAGTGCGTAACGTTTTGCAGCTTATCGCAGACTACAATGGCTTCAA
CCTCGTGGTGTCTGATTCCGTGACTGGTAATGTGACGTTGAGATTGGATGGCGTACCTTGGCAGCAAGTGCTCGATATCA
TTCTTCAAGTGAAAGGTTTAGATAAACGTGTTGAAGGCAATGTTGTGCTCATCGCTCCTCAAGATGAATTGGATTTGCGA
GAGAAGCAGCAGCTTGAGAAAGAGCGTCTGGCGGAAGAGCTTGGCTCCCTCAAATCGGAAATCATCAAAGTCAACTTTGC
CAAAGCGTCAGACATCGCTGAGATGATCAATGGCAAAGAGTCGGTCAGCATGCTGTCAGAGCGTGGTAGTGTGGCGATTG
ATGAACGGACAAACTCTCTTTTGTTGCGTGAGTTACCCGAAAACATCGAAGTGATTCGAGAAATTATCAATTCGTTGGAT
ATCCCAGTCAAACAGGTACAAATTGAAGCGCGAATTGTCACGGTGAACGAAGGCAATCTTGATGAGCTTGGTGTACGTTG
GGGTTTCACCTCCATCAACGGCAGCACAACTGTCGGTGGTTCAATCGAAAACAACCTTGCAACCATTGGTCTCTACAATG
CAGGCAGTGGTAGTGGCAGTGGTGACTCAGGTAGCGGCGGCAGTGGTAGTGAAGGCGGTTCGGCCTCCATTGATGATTTC
CTCAATGTCAATCTTGCTGCGACCTCTGCAAACGCCTCAAGTATTGCTTTCCAAGTGGCTAAATTGGGGTCGGATTTGTT
GTTGGATCTCGAACTCTCTGCACTGCAACGCGAATCAAAAGCGGAAATTATTTCTAGCCCTCGTTTGATCACAACCAATA
AGAAGCCTGCTTATATCGAGCAAGGTACGGAGATCCCTTATCTAGAGTCTTCCTCCAGTGGAGCGACCACGGTTTCATTT
AAGAAAGCCGTGCTTAGCCTGAAAGTGACCCCACAGATCACGCCTGACAATCGTCTCGTGTTGGATTTAAGCGTTACACA
AGATAGACCCGGCGAAATTGTCAAAACAGGCACTGGAGAAGCGGTTGCGATTGATACTCAGCGCATCGGTACACAAGTCT
TGGTCAATAATGGTGAAACGGTAGTGTTAGGCGGTATCTTCCAACACAGTATTACCAATACCACGGACAAGGTTCCTCTG
TTAGGCGATTTACCTTTATTGGGGGCGCTTTTCCGCCGTAGCTATGAAAATGTGGGTAAACGTGAATTGCTGATATTTGT
CACACCCAAGGTTATCTTGCAGTAG

Domains

Predicted by InterproScan.

Bacterial type II/III secretion system short domain (Secretin_N)

(260-325)

Bacterial type II and III secretion system protein (Secretin)

(434-592)

Secretin and TonB N terminus short domain (STN)

(186-234)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A3Q0L3M5

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Vibrio campbellii strain DS40M4

77.966

99.327

0.774
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

73.729

99.327

0.732
  pilQ Vibrio cholerae strain A1552

73.729

99.327

0.732
  pilQ Legionella pneumophila strain ERS1305867

41.156

96.128

0.396
  pilQ Legionella pneumophila strain Lp02

41.429

94.276

0.391
  pilQ Pseudomonas aeruginosa PAK

39.539

94.949

0.375
  comQ Acinetobacter baylyi ADP1

37.162

99.663

0.37

Multiple sequence alignment