Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilM   Type   Machinery gene
Locus tag   FORC53_RS01630 Genome accession   NZ_CP019290
Coordinates   331242..332249 (+) Length   335 a.a.
NCBI ID   WP_017419656.1    Uniprot ID   A0A087I1V6
Organism   Vibrio vulnificus strain FORC_053     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 326242..337249
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  FORC53_RS01615 - 327077..327349 (-) 273 WP_017789760.1 DUF3624 domain-containing protein -
  FORC53_RS01620 (FORC53_0290) oxyR 327623..328522 (+) 900 WP_011079350.1 DNA-binding transcriptional regulator OxyR -
  FORC53_RS01625 (FORC53_0291) - 328589..331102 (-) 2514 WP_118893221.1 penicillin-binding protein 1A -
  FORC53_RS01630 (FORC53_0292) pilM 331242..332249 (+) 1008 WP_017419656.1 type IV pilus assembly protein PilM Machinery gene
  FORC53_RS01635 (FORC53_0293) pilN 332239..332829 (+) 591 WP_011079353.1 PilN domain-containing protein Machinery gene
  FORC53_RS01640 (FORC53_0294) pilO 332822..333412 (+) 591 WP_011079354.1 type 4a pilus biogenesis protein PilO Machinery gene
  FORC53_RS01645 (FORC53_0295) pilP 333402..333914 (+) 513 WP_011079355.1 pilus assembly protein PilP Machinery gene
  FORC53_RS01650 (FORC53_0296) pilQ 333928..335712 (+) 1785 WP_011079356.1 type IV pilus secretin PilQ Machinery gene
  FORC53_RS01655 (FORC53_0297) aroK 335914..336432 (+) 519 WP_011079357.1 shikimate kinase AroK -

Sequence

Protein


Download         Length: 335 a.a.        Molecular weight: 37654.05 Da        Isoelectric Point: 6.3873

>NTDB_id=214574 FORC53_RS01630 WP_017419656.1 331242..332249(+) (pilM) [Vibrio vulnificus strain FORC_053]
MGKPFVTGIDIGHRSLKAVILKPYGEHYALLGYKEIIIKDGIFSDNHTLNHQEIVNSLKELKKELPLFRYQVSLAVPDSS
VISKMLSIDAELDGREREFAILQAFAHQSPFPVDELSIDYVQLVEETSKTNHSYQVYATKKEVVESRVRVAEEVGLKPVL
VDVHAHSLCNILTLAAERHPDKASYCLLDVGYDTTSLVMSRRNEPPLVKEFAYGVSHLNASIGDDLTSPDAHEKTDIFNK
ELTERVRRQLQLFQSMDSQSKVNGLWICGEGAITPLLSETLQRKLSLECEVLNPLALFEQKTAKRKRRIVDWHHFSVAAG
LAIGGIQWLEDSHAA

Nucleotide


Download         Length: 1008 bp        

>NTDB_id=214574 FORC53_RS01630 WP_017419656.1 331242..332249(+) (pilM) [Vibrio vulnificus strain FORC_053]
ATGGGTAAGCCGTTCGTTACCGGAATTGATATTGGTCATCGTAGCCTCAAGGCTGTCATTCTGAAACCTTATGGAGAGCA
CTACGCCCTGTTAGGGTACAAAGAAATAATCATTAAAGACGGTATTTTCTCAGATAACCACACTCTGAATCATCAAGAAA
TTGTCAATAGCCTGAAAGAACTGAAGAAGGAACTGCCTCTTTTCCGTTATCAGGTGTCATTGGCGGTGCCTGATAGTTCA
GTGATTAGTAAGATGTTATCAATCGATGCGGAGTTGGATGGTCGAGAGCGAGAATTCGCTATCCTTCAGGCGTTTGCACA
TCAGTCGCCATTTCCTGTCGATGAATTATCGATTGATTATGTCCAATTGGTCGAAGAAACCAGCAAAACGAATCATAGCT
ATCAAGTTTACGCCACCAAAAAAGAGGTGGTGGAAAGCCGTGTGCGTGTCGCAGAAGAAGTGGGACTGAAACCCGTGCTG
GTAGATGTGCATGCGCATAGCCTGTGCAACATCTTAACGTTAGCGGCAGAGCGCCATCCAGATAAAGCCAGCTATTGCTT
GTTGGATGTGGGTTATGACACCACCTCTTTGGTGATGTCGCGTCGCAACGAACCGCCTTTAGTTAAAGAATTTGCCTATG
GGGTGTCTCACCTCAATGCGTCGATAGGCGACGATCTGACTTCTCCTGATGCTCATGAGAAAACCGACATCTTTAATAAA
GAACTGACAGAGCGAGTTCGTCGGCAATTGCAGCTGTTCCAATCTATGGATAGCCAAAGCAAAGTGAATGGCCTCTGGAT
CTGCGGTGAAGGCGCGATTACTCCGTTGTTGAGTGAAACGTTGCAGAGAAAGCTGAGTTTGGAGTGCGAAGTCCTTAATC
CTCTGGCTCTGTTTGAGCAAAAAACGGCGAAAAGAAAGCGCCGTATTGTTGATTGGCACCATTTTTCGGTGGCAGCGGGA
TTAGCGATTGGTGGCATTCAGTGGCTGGAGGATAGCCATGCAGCATAA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(8-323)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A087I1V6

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilM Vibrio campbellii strain DS40M4

53.134

100

0.531
  pilM Vibrio cholerae strain A1552

48.974

100

0.499

Multiple sequence alignment