Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   BV404_RS01920 Genome accession   NZ_CP019118
Coordinates   398675..400363 (-) Length   562 a.a.
NCBI ID   WP_075988154.1    Uniprot ID   -
Organism   Vibrio fluvialis strain 12605     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 393675..405363
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BV404_RS01895 yacG 394856..395053 (-) 198 WP_020332195.1 DNA gyrase inhibitor YacG -
  BV404_RS01900 zapD 395108..395848 (-) 741 WP_075988151.1 cell division protein ZapD -
  BV404_RS01905 coaE 395881..396489 (-) 609 WP_075988152.1 dephospho-CoA kinase -
  BV404_RS01910 pilD 396492..397361 (-) 870 WP_047459100.1 prepilin peptidase Machinery gene
  BV404_RS01915 pilC 397413..398639 (-) 1227 WP_075988153.1 type II secretion system F family protein Machinery gene
  BV404_RS01920 pilB 398675..400363 (-) 1689 WP_075988154.1 type IV-A pilus assembly ATPase PilB Machinery gene
  BV404_RS01925 - 400363..400791 (-) 429 WP_044364133.1 pilin -
  BV404_RS01930 nadC 401023..401910 (-) 888 WP_044364132.1 carboxylating nicotinate-nucleotide diphosphorylase -
  BV404_RS01935 ampD 402064..402615 (+) 552 WP_032081227.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  BV404_RS01940 fldB 402620..403141 (-) 522 WP_020430452.1 flavodoxin FldB -
  BV404_RS01945 xerD 403282..404190 (+) 909 WP_020331983.1 site-specific tyrosine recombinase XerD -
  BV404_RS01950 dsbC 404207..404971 (+) 765 WP_020430455.1 bifunctional protein-disulfide isomerase/oxidoreductase DsbC -

Sequence

Protein


Download         Length: 562 a.a.        Molecular weight: 62017.95 Da        Isoelectric Point: 6.1972

>NTDB_id=213263 BV404_RS01920 WP_075988154.1 398675..400363(-) (pilB) [Vibrio fluvialis strain 12605]
MLTNLLAILRQADLLSPTQEQAVIDHIHASGTNVPEALIDLAIFNHHDLVEHISQIFGLPNTELTRFDFAPLCQQLGLRE
LITRYQALPLNSNGQILTLAVSDPTHLQVEDEFRFATGLQVELVLADHKALQAAIRRLYGRAISSHGTQGKEISQDELAS
LVQVSDDEKTSIEDLSQDDSPVSRFINQILLDAVRKGASDIHFEPYESFYRIRLRCDGILVEIQQPANHLSRRLAARIKI
LSKLDIAERRLPQDGRIKLRLNADTAIDMRVSTLPTLWGEKIVLRLLDSSASSLDIDKLGYNDAQKQLYLAALTKPQGMI
LMTGPTGSGKTVSLYTGLRILNTDEVNISTAEDPVEINLPGINQVQVQPKIGFGFAQALRSFLRQDPDVVMVGEIRDLET
AEIAVKAAQTGHLVLSTLHTNSAAETVIRLANMGIEPFNLASSLSLIIAQRLARRLCPHCKVATSPSPTLQQQFHLSSQQ
PIYAANPSGCNECTNGYSGRVGIYEVMAFNPALAEAIMQRASIHQIESIAQQHGMQTLLQSGIEKLRAGITSFSELQRVL
YF

Nucleotide


Download         Length: 1689 bp        

>NTDB_id=213263 BV404_RS01920 WP_075988154.1 398675..400363(-) (pilB) [Vibrio fluvialis strain 12605]
ATGCTCACCAACCTACTCGCCATCCTGCGTCAGGCCGATTTGCTTAGTCCTACGCAGGAACAGGCTGTGATTGATCATAT
TCACGCTTCCGGGACCAATGTTCCGGAGGCGTTGATCGATTTGGCGATCTTTAATCATCACGATCTGGTCGAACATATCA
GCCAGATCTTTGGTTTGCCGAATACTGAACTGACCCGTTTTGACTTCGCGCCGCTGTGCCAGCAGTTGGGGCTGCGCGAG
CTCATCACCCGTTATCAGGCACTGCCCCTCAATAGCAACGGTCAGATACTGACTCTTGCGGTATCCGATCCTACTCACCT
ACAAGTTGAAGACGAGTTTCGCTTTGCGACCGGGTTACAAGTAGAACTGGTATTGGCTGATCATAAGGCACTGCAAGCTG
CTATTCGCCGCTTGTATGGCCGAGCCATCTCGTCACATGGCACGCAGGGCAAGGAGATCAGCCAGGACGAACTGGCCAGT
CTGGTGCAGGTTTCCGATGACGAAAAAACATCAATCGAAGATCTGAGTCAGGATGACTCCCCGGTCAGTCGTTTCATCAA
CCAGATCCTGCTGGACGCGGTGCGTAAAGGCGCTTCTGATATCCACTTTGAACCCTATGAATCCTTTTATCGCATTCGCC
TGCGCTGCGACGGTATTTTGGTGGAAATCCAGCAACCAGCCAACCATTTAAGCCGCCGACTGGCTGCACGGATTAAGATT
TTGTCGAAACTCGATATCGCCGAACGACGTTTACCCCAGGACGGCCGCATCAAATTACGCCTCAATGCGGACACTGCCAT
TGATATGCGGGTCTCCACTCTGCCAACCTTGTGGGGGGAGAAAATCGTCCTGCGTCTGCTCGACAGCAGTGCGTCCAGCC
TCGATATCGACAAGTTGGGTTATAACGATGCGCAAAAGCAGCTTTATCTGGCGGCCCTCACCAAGCCGCAAGGCATGATT
CTGATGACTGGCCCGACGGGCAGCGGTAAGACAGTCTCACTCTATACCGGCCTTCGTATTCTCAATACCGACGAAGTGAA
TATTTCTACCGCCGAAGATCCGGTGGAAATTAACCTGCCCGGCATTAACCAGGTTCAGGTGCAGCCCAAAATTGGTTTTG
GCTTTGCGCAGGCTCTGCGCTCCTTTCTGCGTCAGGATCCCGATGTGGTGATGGTGGGCGAAATCCGCGATTTAGAAACG
GCAGAGATTGCCGTCAAAGCCGCCCAAACCGGCCACTTGGTGCTATCCACACTGCACACCAACTCCGCCGCCGAAACCGT
GATTCGGCTGGCGAACATGGGCATTGAACCGTTCAACCTCGCTTCATCGCTGAGCCTCATCATCGCTCAACGACTTGCCC
GGCGTCTTTGCCCTCATTGCAAAGTCGCCACATCGCCCTCTCCGACACTGCAGCAACAATTTCACTTGAGTTCTCAGCAA
CCTATCTATGCCGCCAACCCATCAGGCTGCAACGAGTGCACCAACGGTTACAGCGGGCGGGTGGGGATTTATGAAGTGAT
GGCATTTAACCCCGCGCTGGCTGAAGCGATTATGCAACGAGCCAGCATCCATCAAATCGAATCCATTGCCCAGCAACATG
GCATGCAAACGCTGTTGCAATCGGGGATCGAAAAACTTCGCGCCGGGATCACCAGTTTCAGTGAGTTGCAGCGTGTTCTT
TATTTCTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(188-455)

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(39-141)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Vibrio cholerae strain A1552

82.028

100

0.82
  pilB Vibrio parahaemolyticus RIMD 2210633

72.42

100

0.724
  pilB Vibrio campbellii strain DS40M4

72.242

100

0.722
  pilB Legionella pneumophila strain ERS1305867

52.418

91.993

0.482
  pilB Acinetobacter baumannii D1279779

50.867

92.349

0.47
  pilB Acinetobacter baylyi ADP1

49.146

93.772

0.461
  pilF Neisseria gonorrhoeae MS11

44.681

100

0.448
  pilF Thermus thermophilus HB27

37.388

99.466

0.372
  pilB/pilB1 Synechocystis sp. PCC 6803

37.455

99.288

0.372

Multiple sequence alignment