Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilU   Type   Machinery gene
Locus tag   BVL33_RS01390 Genome accession   NZ_CP019041
Coordinates   283825..285000 (+) Length   391 a.a.
NCBI ID   WP_004914541.1    Uniprot ID   S7Y0P3
Organism   Acinetobacter junii strain 65     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 278825..290000
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BVL33_RS01365 (BVL33_01365) - 279489..279962 (-) 474 WP_004914520.1 hypothetical protein -
  BVL33_RS01370 (BVL33_01370) guaD 280148..281473 (+) 1326 WP_075695976.1 guanine deaminase -
  BVL33_RS01375 (BVL33_01375) hpt 281690..282217 (+) 528 WP_004914531.1 hypoxanthine phosphoribosyltransferase -
  BVL33_RS01380 (BVL33_01380) - 282446..282697 (+) 252 WP_004914533.1 GlsB/YeaQ/YmgE family stress response membrane protein -
  BVL33_RS01385 (BVL33_01385) - 282747..283445 (-) 699 WP_039047176.1 DsbC family protein -
  BVL33_RS01390 (BVL33_01390) pilU 283825..285000 (+) 1176 WP_004914541.1 PilT/PilU family type 4a pilus ATPase Machinery gene
  BVL33_RS01395 (BVL33_01395) mnmE 285056..286411 (-) 1356 WP_075696868.1 tRNA uridine-5-carboxymethylaminomethyl(34) synthesis GTPase MnmE -
  BVL33_RS01400 (BVL33_01400) yidC 286502..288259 (-) 1758 WP_004914555.1 membrane protein insertase YidC -
  BVL33_RS01405 (BVL33_01405) yidD 288265..288585 (-) 321 WP_004950001.1 membrane protein insertion efficiency factor YidD -
  BVL33_RS01410 (BVL33_01410) rnpA 288595..288990 (-) 396 WP_004914561.1 ribonuclease P protein component -
  BVL33_RS01415 (BVL33_01415) rpmH 289019..289153 (-) 135 WP_000831329.1 50S ribosomal protein L34 -

Sequence

Protein


Download         Length: 391 a.a.        Molecular weight: 44323.84 Da        Isoelectric Point: 6.3220

>NTDB_id=212797 BVL33_RS01390 WP_004914541.1 283825..285000(+) (pilU) [Acinetobacter junii strain 65]
MYTTELLEEAKTFMFHMLTKVVEYGGSDLFITADFPPSIKLQGLMRPLGQQALTAEKTKLFAYSLMNDKQRLDFENDWEC
NFAINVPNVSRFRVNVFKQQLQIGMVIRTITSEIPTFQKLKLPESLKNVIMEKRGLVLVVGGTGSGKSTSLAAMIDHRNE
NSAGHIITVEDPVEYVHKHKKSMITHREVGVDCHTWHHALKNTLRQAPDVILIGEIRDTETMEHAIAFAETGHLCLGTLH
ANNANQALDRIINFFPEERRNQLLMDLSSNMKAIISQRLVRTQDGQGRRAAIEILLNTPLIADNILKGQFHVLKEIMTKS
RELGMQTFDQALFDLYNEGAISYDEALRNADSVNELRLQIKLKSSRQDGMPVSTTSLNMVKEEEVEEQEEA

Nucleotide


Download         Length: 1176 bp        

>NTDB_id=212797 BVL33_RS01390 WP_004914541.1 283825..285000(+) (pilU) [Acinetobacter junii strain 65]
ATGTATACCACTGAATTATTAGAAGAAGCCAAAACCTTTATGTTTCATATGCTCACTAAAGTAGTTGAGTATGGGGGATC
TGATTTATTTATTACCGCAGATTTCCCACCTAGCATTAAGTTACAGGGTTTGATGCGCCCTTTAGGACAACAAGCGCTTA
CTGCAGAAAAAACCAAGTTGTTTGCTTATAGTTTAATGAATGACAAACAACGTCTAGACTTCGAAAATGATTGGGAATGT
AACTTTGCCATTAATGTCCCGAATGTTTCAAGGTTTCGGGTCAATGTTTTCAAACAACAATTACAAATAGGCATGGTGAT
TCGAACCATTACGTCTGAAATTCCAACCTTTCAAAAACTAAAACTCCCAGAATCACTAAAAAATGTGATTATGGAAAAAC
GAGGTTTGGTACTGGTGGTTGGCGGAACAGGATCAGGAAAATCAACCTCTTTAGCAGCCATGATTGATCATCGAAATGAG
AACTCTGCTGGACATATCATTACCGTTGAAGATCCTGTTGAATACGTCCATAAACACAAAAAATCCATGATCACGCACCG
TGAGGTTGGAGTTGATTGTCATACATGGCATCACGCGTTGAAAAATACGCTACGCCAAGCACCAGATGTGATTTTAATTG
GTGAAATACGTGATACTGAAACGATGGAACATGCGATTGCTTTTGCTGAAACAGGGCATCTATGTTTGGGAACACTGCAC
GCCAACAATGCCAATCAGGCACTGGATCGTATTATTAACTTTTTCCCAGAGGAACGTCGCAATCAACTGTTGATGGATCT
CTCATCCAATATGAAAGCGATTATTTCTCAACGTCTCGTGCGAACTCAAGATGGTCAAGGTCGTCGAGCTGCCATTGAAA
TCCTCCTCAATACGCCATTAATCGCAGACAATATTTTGAAAGGGCAATTCCATGTGCTCAAAGAGATCATGACCAAATCT
CGCGAGTTAGGTATGCAGACCTTTGATCAGGCTTTGTTTGATCTATATAACGAAGGTGCAATTAGTTACGATGAAGCATT
GCGTAACGCCGACTCTGTCAATGAGTTACGCTTGCAAATTAAATTAAAAAGTTCACGCCAAGATGGGATGCCGGTTTCAA
CCACTTCACTGAACATGGTCAAAGAAGAGGAAGTTGAGGAGCAAGAAGAAGCTTAA

Domains

Predicted by InterproScan.

Type II/IV secretion system protein (T2SSE)

(116-282)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB S7Y0P3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilU Pseudomonas stutzeri DSM 10701

58.197

93.606

0.545
  pilU Acinetobacter baylyi ADP1

55.62

88.747

0.494
  pilU Vibrio cholerae strain A1552

52.601

88.491

0.465
  pilT Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

43.284

85.678

0.371
  pilT Acinetobacter nosocomialis M2

44.172

83.376

0.368
  pilT Acinetobacter baumannii D1279779

44.172

83.376

0.368
  pilT Acinetobacter baumannii strain A118

44.172

83.376

0.368
  pilT Acinetobacter baylyi ADP1

42.56

85.934

0.366

Multiple sequence alignment