Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   AHTJS_RS14865 Genome accession   NZ_CP018871
Coordinates   3021659..3023374 (+) Length   571 a.a.
NCBI ID   WP_075316303.1    Uniprot ID   A0A1L6KQY3
Organism   Acinetobacter haemolyticus strain TJS01     
Function   power the assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3016659..3028374
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  AHTJS_RS14830 (AHTJS_14815) nusA 3017468..3018952 (-) 1485 WP_004637688.1 transcription termination factor NusA -
  AHTJS_RS14835 (AHTJS_14820) rimP 3018990..3019514 (-) 525 WP_005083847.1 ribosome maturation factor RimP -
  AHTJS_RS14855 (AHTJS_14840) secG 3020150..3020479 (-) 330 WP_004637686.1 preprotein translocase subunit SecG -
  AHTJS_RS14860 (AHTJS_14845) tpiA 3020492..3021283 (-) 792 WP_075316302.1 triose-phosphate isomerase -
  AHTJS_RS14865 (AHTJS_14850) pilB 3021659..3023374 (+) 1716 WP_075316303.1 type IV-A pilus assembly ATPase PilB Machinery gene
  AHTJS_RS14870 (AHTJS_14855) pilC 3023399..3024625 (+) 1227 WP_005083853.1 type II secretion system F family protein Machinery gene
  AHTJS_RS14875 (AHTJS_14860) pilD 3024625..3025485 (+) 861 WP_075316304.1 prepilin peptidase Machinery gene
  AHTJS_RS14880 (AHTJS_14865) coaE 3025487..3026092 (+) 606 WP_005088002.1 dephospho-CoA kinase -
  AHTJS_RS14885 (AHTJS_14870) - 3026089..3027000 (-) 912 WP_075316305.1 DMT family transporter -
  AHTJS_RS14895 (AHTJS_14880) rlmB 3027040..3027786 (-) 747 WP_004637678.1 23S rRNA (guanosine(2251)-2'-O)-methyltransferase RlmB -
  AHTJS_RS14900 (AHTJS_14885) - 3027895..3028221 (-) 327 WP_004637677.1 pyrimidine/purine nucleoside phosphorylase -

Sequence

Protein


Download         Length: 571 a.a.        Molecular weight: 63291.48 Da        Isoelectric Point: 5.1457

>NTDB_id=211177 AHTJS_RS14865 WP_075316303.1 3021659..3023374(+) (pilB) [Acinetobacter haemolyticus strain TJS01]
MSGLHTSPKFSGFIRRLVEDGHMSASTMQTAIDTAKKTQQDIVGYLIEHHRISQLTIAESISAEFGEPIFDLAVFDTALL
PRELADSKLIQKHRVAPLIQRGQILYVATSNPTNIDALDAMRFNTKLNIEPIIVEHSKLEKLIEQQFGDSGSFDFGDSED
FDLDIDVSQDSQKDESDETPQGDESPIVKYINKLLIDAIRMGASDLHFEPYEKTYRVRYRVDGVLRQIATPPLQMANRLA
SRLKVMSQMDISEKRMPQDGRIKLKLSKTKAIDFRVNSLPTLFGEKLVLRILDPSSAMLGIEALGYEDEQKALFLEALDK
PQGMLLITGPTGSGKTVSLYTGLNILNTEDTNISTAEDPVEINLEGINQVNVNTKTGLTFSAALKAFLRQDPDIIMVGEI
RDLETAEIAIKAAQTGHMVMSTLHTNSAPETLTRLRNMGVPSFNIATSVNLVIAQRLARRLCSHCKAPIEIPKQSLLELG
FTEQDLADPELQIFQPVGCSECREGYKGRVGIYEVMKVTPEISKIIMEDGNALQIAAASEKAGFNNLRRSGLKKVMQGIT
SLQEINRVTSE

Nucleotide


Download         Length: 1716 bp        

>NTDB_id=211177 AHTJS_RS14865 WP_075316303.1 3021659..3023374(+) (pilB) [Acinetobacter haemolyticus strain TJS01]
ATGTCAGGATTACATACGTCTCCAAAATTTTCGGGGTTTATTCGACGGTTGGTCGAAGATGGTCATATGTCTGCATCGAC
AATGCAAACTGCAATCGATACAGCAAAAAAAACACAACAAGATATTGTTGGATATTTAATTGAACATCATCGTATCTCTC
AACTGACCATTGCAGAGTCAATCTCGGCAGAGTTTGGCGAACCTATTTTTGATTTAGCTGTTTTTGATACTGCTTTATTA
CCTCGTGAGCTTGCTGACAGCAAATTAATTCAAAAGCATCGGGTTGCCCCTCTCATTCAACGCGGGCAAATTCTTTATGT
CGCGACAAGCAATCCAACCAATATCGACGCGCTTGATGCGATGCGATTTAACACCAAACTCAATATTGAACCGATTATCG
TCGAACATAGTAAACTTGAAAAACTGATTGAACAGCAGTTTGGTGACTCTGGAAGTTTTGATTTTGGTGACAGTGAAGAC
TTTGACTTAGATATTGATGTCAGCCAAGATTCGCAAAAAGATGAAAGTGATGAAACGCCTCAAGGGGATGAATCACCAAT
TGTAAAGTACATCAACAAGTTACTGATTGATGCCATTCGCATGGGTGCGTCTGATCTACATTTTGAACCCTATGAAAAAA
CCTATCGTGTTCGTTATCGCGTAGATGGTGTCTTAAGACAAATTGCGACGCCACCGTTGCAAATGGCAAATCGCTTAGCT
TCCCGTTTAAAAGTCATGTCTCAAATGGACATTTCTGAAAAACGAATGCCGCAAGATGGTCGTATTAAGCTCAAACTTTC
GAAAACCAAAGCGATTGACTTTCGTGTCAACTCACTGCCTACCCTATTTGGTGAAAAGCTAGTACTCCGTATTCTGGATC
CTTCCAGCGCCATGCTCGGAATTGAAGCCTTAGGCTATGAAGATGAACAAAAGGCGTTATTCCTAGAAGCATTGGATAAA
CCTCAAGGCATGCTGTTAATTACTGGCCCAACAGGTTCTGGTAAAACTGTGTCGTTATATACAGGTCTCAACATTCTAAA
TACTGAAGACACCAATATTTCTACTGCTGAAGACCCAGTGGAGATTAATTTAGAAGGAATCAATCAGGTTAACGTCAATA
CTAAGACAGGCCTTACCTTCTCTGCCGCGCTGAAAGCATTCTTACGTCAAGATCCAGACATTATCATGGTCGGTGAGATT
CGTGACCTAGAAACAGCAGAAATTGCAATCAAGGCTGCTCAAACGGGTCATATGGTGATGTCCACGCTACATACCAATAG
TGCACCTGAAACACTGACGCGTTTACGCAATATGGGCGTTCCCTCTTTCAATATCGCAACTTCGGTCAATCTTGTGATTG
CACAACGCTTAGCCCGTCGTTTATGCTCACACTGTAAAGCACCCATAGAGATCCCCAAACAAAGCTTATTAGAACTCGGC
TTTACTGAACAGGATTTAGCTGACCCTGAATTGCAAATCTTTCAACCAGTCGGCTGCTCAGAATGTCGTGAAGGCTATAA
AGGCCGTGTCGGTATCTATGAGGTCATGAAGGTGACGCCTGAGATCTCTAAAATTATTATGGAGGATGGCAATGCTTTAC
AAATCGCAGCGGCATCAGAAAAAGCAGGATTTAATAATTTAAGACGCTCAGGTTTAAAGAAGGTCATGCAAGGTATTACC
TCATTGCAAGAAATTAACCGCGTCACAAGTGAATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(46-149)

Type II/IV secretion system protein (T2SSE)

(193-460)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1L6KQY3

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Acinetobacter baumannii D1279779

82.137

100

0.821
  pilB Acinetobacter baylyi ADP1

77.408

100

0.774
  pilB Legionella pneumophila strain ERS1305867

53.846

93.345

0.503
  pilF Neisseria gonorrhoeae MS11

48.696

100

0.49
  pilB Vibrio parahaemolyticus RIMD 2210633

49.195

97.898

0.482
  pilB Vibrio cholerae strain A1552

48.825

96.848

0.473
  pilB Vibrio campbellii strain DS40M4

51.252

90.893

0.466
  pilF Thermus thermophilus HB27

37.478

98.599

0.37
  pilB/pilB1 Synechocystis sp. PCC 6803

33.659

100

0.363

Multiple sequence alignment