Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   BUQ73_RS24645 Genome accession   NZ_CP018846
Coordinates   5447875..5449143 (-) Length   422 a.a.
NCBI ID   WP_079230046.1    Uniprot ID   -
Organism   Pseudomonas putida strain AA7     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 5442875..5454143
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BUQ73_RS24630 - 5444513..5446144 (-) 1632 WP_079230044.1 AAA family ATPase -
  BUQ73_RS24635 aroB 5446160..5447257 (-) 1098 WP_079230045.1 3-dehydroquinate synthase -
  BUQ73_RS24640 aroK 5447352..5447870 (-) 519 WP_027920998.1 shikimate kinase AroK -
  BUQ73_RS24645 pilQ 5447875..5449143 (-) 1269 WP_079230046.1 type IV pilus secretin PilQ Machinery gene
  BUQ73_RS24650 - 5449067..5450083 (-) 1017 WP_237772730.1 pilus assembly protein PilP -
  BUQ73_RS24655 - 5450080..5450616 (-) 537 WP_079230047.1 PilN domain-containing protein -
  BUQ73_RS24660 pilM 5450616..5451470 (-) 855 WP_079230048.1 type IV pilus biogenesis protein PilM -
  BUQ73_RS24665 - 5451647..5454100 (+) 2454 WP_079230049.1 penicillin-binding protein 1A -

Sequence

Protein


Download         Length: 422 a.a.        Molecular weight: 45949.74 Da        Isoelectric Point: 4.8681

>NTDB_id=210983 BUQ73_RS24645 WP_079230046.1 5447875..5449143(-) (pilQ) [Pseudomonas putida strain AA7]
MKCLLMWMAVALIPSQWCMAAPYRGEPISLNFQDVEVRSVLQVLADYAGVNLVASDTVQGNVTLRLHEVPWDQALELVLR
SKGLARREEGNVLLVAPAAELAEQSRGARIGQELDAQLQPLRRELLPIHHANASELAELLLATLADDGILTGRGSLSVDA
RTNTLVAHQPAERLAELRQLVAQLDVPVRQVEIEARIVEANVDYEKGLGVRWGRRLYGEAPQLGKDLFVDLGMERGGASI
GVGLLRGGVLLDLELSAMEKSGNGEIISQPKVVTADKETARILKGTEVPYQETSTSGATSVAFREASLSLEVTPQITPDG
KVIMTVKVTKDEPDFVNALNDVPPIRKNEVNAKVSVADGETIVIGGVYSTTQNNVVDKVPFFGDLPYVGRLFRRDALQEK
KSELLVFLTPRIMSDQAIAVSR

Nucleotide


Download         Length: 1269 bp        

>NTDB_id=210983 BUQ73_RS24645 WP_079230046.1 5447875..5449143(-) (pilQ) [Pseudomonas putida strain AA7]
ATGAAATGCCTGTTGATGTGGATGGCGGTGGCCCTGATACCCAGTCAGTGGTGCATGGCAGCACCCTATCGGGGTGAGCC
CATATCGCTGAATTTCCAGGATGTCGAGGTGCGCTCGGTGCTTCAGGTGCTGGCTGACTACGCCGGGGTCAACCTGGTGG
CCAGCGATACGGTGCAAGGCAATGTCACCTTGCGGCTGCACGAAGTACCCTGGGATCAGGCGCTGGAGCTGGTCCTTCGC
AGCAAGGGGCTGGCGCGGCGCGAGGAGGGGAATGTGCTGCTGGTTGCACCTGCAGCCGAGCTGGCCGAGCAATCCCGCGG
CGCCCGTATCGGGCAGGAACTGGATGCGCAACTGCAGCCGTTGCGACGAGAGCTGCTGCCGATTCATCACGCCAACGCTT
CCGAACTTGCAGAGCTGCTGCTGGCAACACTGGCGGATGACGGCATTCTGACCGGTCGTGGCAGCTTGAGCGTCGATGCG
CGCACCAACACCTTGGTGGCGCATCAGCCCGCAGAACGCCTGGCCGAGTTGCGGCAGCTGGTCGCGCAACTTGACGTGCC
GGTACGCCAGGTGGAGATCGAGGCGCGCATCGTGGAGGCCAATGTCGACTACGAGAAAGGCCTGGGCGTGCGTTGGGGGC
GGCGGTTATATGGGGAAGCGCCGCAACTGGGCAAGGATCTGTTCGTCGACCTGGGTATGGAGCGGGGTGGTGCCAGTATC
GGTGTAGGCCTTTTGCGCGGCGGCGTGTTGCTGGACCTGGAGCTCAGTGCGATGGAAAAGAGCGGCAACGGAGAAATCAT
CTCCCAACCCAAGGTGGTCACGGCCGACAAGGAAACGGCCAGAATTCTGAAGGGCACCGAGGTGCCATATCAGGAAACCA
GCACGAGTGGCGCCACCTCTGTGGCCTTTCGCGAGGCGTCGCTGTCGCTGGAAGTCACGCCACAGATCACTCCGGACGGC
AAGGTCATCATGACAGTCAAGGTGACCAAGGATGAACCTGATTTCGTCAATGCCCTGAATGACGTGCCGCCGATTCGCAA
GAACGAGGTCAATGCCAAGGTCAGTGTTGCGGACGGTGAGACCATCGTCATCGGTGGCGTGTATTCGACCACGCAAAACA
ATGTGGTCGACAAGGTGCCATTTTTCGGCGATCTGCCGTATGTTGGGCGGCTGTTTCGACGCGATGCATTACAAGAGAAA
AAATCCGAGCTGCTGGTCTTCCTGACTCCGCGTATCATGAGTGACCAGGCGATTGCTGTGAGTCGTTGA

Domains

Predicted by InterproScan.

Bacterial type II and III secretion system protein (Secretin)

(257-413)

Bacterial type II/III secretion system short domain (Secretin_N)

(125-190)

Secretin and TonB N terminus short domain (STN)

(51-97)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

58.716

100

0.607
  comQ Acinetobacter baylyi ADP1

42.511

100

0.457
  pilQ Acinetobacter baumannii strain A118

40.611

100

0.441
  pilQ Acinetobacter baumannii D1279779

40.611

100

0.441
  pilQ Legionella pneumophila strain Lp02

42.823

99.052

0.424
  pilQ Legionella pneumophila strain ERS1305867

42.823

99.052

0.424
  pilQ Vibrio campbellii strain DS40M4

41.135

100

0.412
  pilQ Vibrio cholerae O1 biovar El Tor strain E7946

41.446

98.341

0.408
  pilQ Vibrio cholerae strain A1552

41.446

98.341

0.408

Multiple sequence alignment