Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilQ   Type   Machinery gene
Locus tag   PFAS1_RS09125 Genome accession   NZ_CP018319
Coordinates   1964484..1966574 (-) Length   696 a.a.
NCBI ID   WP_076028936.1    Uniprot ID   -
Organism   Pseudomonas frederiksbergensis strain AS1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1959484..1971574
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PFAS1_RS09110 (PFAS1_09100) - 1961094..1962698 (-) 1605 WP_076028935.1 AAA family ATPase -
  PFAS1_RS09115 (PFAS1_09105) aroB 1962709..1963809 (-) 1101 WP_033055371.1 3-dehydroquinate synthase -
  PFAS1_RS09120 (PFAS1_09110) aroK 1963961..1964479 (-) 519 WP_010464421.1 shikimate kinase AroK -
  PFAS1_RS09125 (PFAS1_09115) pilQ 1964484..1966574 (-) 2091 WP_076028936.1 type IV pilus secretin PilQ Machinery gene
  PFAS1_RS09130 (PFAS1_09120) - 1966588..1967115 (-) 528 WP_076028937.1 pilus assembly protein PilP -
  PFAS1_RS09135 (PFAS1_09125) pilO 1967112..1967735 (-) 624 WP_076028938.1 type 4a pilus biogenesis protein PilO -
  PFAS1_RS09140 (PFAS1_09130) - 1967732..1968298 (-) 567 WP_076028939.1 PilN domain-containing protein -
  PFAS1_RS09145 (PFAS1_09135) comM 1968298..1969362 (-) 1065 WP_076028940.1 pilus assembly protein PilM Machinery gene

Sequence

Protein


Download         Length: 696 a.a.        Molecular weight: 75209.47 Da        Isoelectric Point: 6.7227

>NTDB_id=208024 PFAS1_RS09125 WP_076028936.1 1964484..1966574(-) (pilQ) [Pseudomonas frederiksbergensis strain AS1]
MNRIFSTFGTSLWIALLSPMVLAANLEALDVAALPGDRVELKLSFDGPPPSPQGYTTQSPARIALDLPGVVSQLKSKNRE
LGGGNARSATVVQAKDRTRLIINLTQLTPYNARVEGNTLLVVVGQGANKTASSPAPKAPRAATATPAPAKAYAPAGKAIR
GVDFQRGTQGEGNVVIDLSDPSIAPDIQERDGKIILGFPKTRLPEPLRVRLDVKDFATPVQFVNASATGDRATISIEPSG
AFDYSTYQTDNKLTVSIRPMTADDLQKRNTERFAYTGEKLSLNFQDIDVRSVLQLIADFTNLNLVASDTVQGGITLRLQN
VPWDQALDLVLKTKGLDKRKIGNVLLVAPADEIAARERQELESQKQIADLAPLRRELLQVNYAKAADIAKLFQSVTSVES
KVDERGSITVDERTNNIIAYQTQNRLDELRRIVAQLDIPVRQVMIEARIVEANVDYDKSLGVRWGGSIQNKGNWNTSGVS
NSGANASSTIGTPGSTSTNSPFVDLGAAGNTSGLGIAFITDNVLLDLELTAMEKTGNGEIVSQPKVVTSDKETAKILKGT
EIPYQEASSSGATSVSFKEASLSLEVTPQITPDNRIIMEVKVTKDEPDYLNKVQDVPPIKKNEVNAKVLVNDGETIVIGG
VFSNTQSKVVDKVPFLGDVPYLGRLFRRDVVSEKKSELLVFLTPRIMNNQAIAVSR

Nucleotide


Download         Length: 2091 bp        

>NTDB_id=208024 PFAS1_RS09125 WP_076028936.1 1964484..1966574(-) (pilQ) [Pseudomonas frederiksbergensis strain AS1]
ATGAACAGGATTTTCTCAACCTTCGGTACTTCGCTATGGATAGCGTTGCTGTCGCCGATGGTACTAGCGGCCAACCTTGA
AGCGCTGGATGTTGCGGCGTTGCCGGGGGACCGCGTCGAGTTGAAGTTGTCGTTCGATGGGCCACCACCGTCGCCTCAGG
GTTACACCACGCAATCGCCGGCACGGATTGCGCTGGATCTGCCTGGTGTCGTCAGTCAGTTGAAAAGCAAAAATCGTGAA
CTGGGCGGCGGAAATGCCCGCAGCGCCACGGTGGTGCAAGCCAAGGACCGTACGCGGCTGATCATCAATCTGACCCAGTT
GACGCCGTACAACGCACGGGTCGAAGGCAACACGCTGTTGGTGGTAGTCGGGCAAGGTGCCAACAAAACGGCCTCCAGCC
CGGCGCCCAAAGCGCCGCGTGCAGCGACTGCAACGCCAGCCCCGGCGAAAGCCTATGCGCCGGCAGGCAAGGCCATCCGG
GGTGTGGATTTCCAGCGCGGCACCCAAGGCGAGGGCAATGTGGTCATCGATCTGTCGGACCCTTCCATTGCCCCGGACAT
TCAGGAGCGCGACGGCAAGATCATTCTCGGTTTCCCCAAGACCCGATTGCCTGAACCGTTACGTGTTCGTCTGGACGTCA
AGGATTTCGCCACCCCGGTGCAGTTCGTCAACGCCAGCGCCACGGGCGATCGGGCCACGATCAGCATTGAACCCAGCGGC
GCCTTTGATTATTCGACTTACCAGACCGACAACAAGCTGACCGTCAGCATCCGGCCGATGACCGCCGATGACTTGCAGAA
ACGCAACACCGAACGCTTCGCCTACACCGGTGAAAAGCTCTCGCTGAATTTCCAGGACATCGATGTGCGTTCGGTGCTGC
AACTGATCGCCGATTTCACCAACCTCAATCTGGTGGCCAGCGACACGGTGCAGGGCGGCATCACCCTGCGCTTGCAGAAC
GTACCGTGGGATCAGGCGCTGGATCTGGTGTTGAAAACCAAGGGCCTGGATAAACGCAAGATTGGTAACGTTTTGCTGGT
GGCGCCGGCCGATGAAATTGCCGCCCGCGAGCGTCAGGAACTGGAGTCGCAAAAGCAGATAGCTGACCTGGCGCCCCTGC
GTCGCGAACTGTTGCAAGTCAACTACGCCAAAGCTGCCGATATCGCCAAGCTGTTCCAGTCGGTCACCAGTGTCGAGTCG
AAAGTCGACGAGCGGGGCTCGATCACCGTGGATGAGCGTACCAACAACATCATTGCCTACCAGACCCAGAACCGCCTCGA
CGAACTGCGCCGTATCGTCGCGCAACTGGATATCCCGGTGCGTCAGGTGATGATCGAAGCACGGATCGTTGAAGCCAACG
TCGACTATGACAAAAGCCTCGGCGTGCGCTGGGGCGGATCGATCCAGAACAAGGGCAACTGGAACACCTCGGGTGTCAGT
AATAGTGGGGCGAATGCTTCATCGACCATCGGTACGCCTGGCAGCACCAGCACCAACTCGCCATTCGTCGATCTGGGTGC
CGCCGGCAATACCTCGGGGCTCGGGATCGCCTTCATCACCGACAATGTGTTGCTGGATCTCGAGCTGACGGCCATGGAGA
AGACCGGCAACGGCGAAATCGTCTCGCAGCCCAAGGTGGTCACCTCGGACAAGGAGACCGCGAAAATCCTCAAGGGCACG
GAGATTCCCTATCAGGAAGCCAGCTCCAGCGGCGCAACGTCTGTGTCGTTCAAGGAGGCCTCGCTGTCTCTGGAAGTGAC
GCCACAGATCACGCCGGACAACCGGATCATCATGGAGGTCAAGGTCACCAAGGACGAACCGGACTACCTGAACAAGGTGC
AGGATGTGCCGCCGATCAAGAAAAACGAGGTCAATGCCAAGGTCCTGGTCAACGACGGCGAGACCATCGTCATTGGCGGG
GTTTTCTCAAATACTCAGAGCAAGGTTGTAGATAAGGTGCCATTTCTCGGCGATGTGCCGTATCTTGGCCGCCTTTTCCG
GCGTGACGTGGTTTCGGAGAAAAAATCCGAGCTGTTGGTGTTTCTCACACCGCGTATCATGAATAACCAGGCGATTGCTG
TGAGTCGTTGA

Domains

Predicted by InterproScan.

Bacterial type II and III secretion system protein (Secretin)

(531-687)

Secretin and TonB N terminus short domain (STN)

(303-349)

AMIN domain (AMIN)

(37-121)

Bacterial type II/III secretion system short domain (Secretin_N)

(377-442)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilQ Pseudomonas aeruginosa PAK

73.427

100

0.754
  comQ Acinetobacter baylyi ADP1

41.512

100

0.418
  pilQ Acinetobacter baumannii strain A118

38.4

100

0.414
  pilQ Acinetobacter baumannii D1279779

38.4

100

0.414
  pilQ Legionella pneumophila strain Lp02

38.109

97.27

0.371
  pilQ Legionella pneumophila strain ERS1305867

37.834

96.839

0.366

Multiple sequence alignment