Detailed information    

insolico Bioinformatically predicted

Overview

Name   comM   Type   Machinery gene
Locus tag   PFAS1_RS09145 Genome accession   NZ_CP018319
Coordinates   1968298..1969362 (-) Length   354 a.a.
NCBI ID   WP_076028940.1    Uniprot ID   -
Organism   Pseudomonas frederiksbergensis strain AS1     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1963298..1974362
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  PFAS1_RS09120 (PFAS1_09110) aroK 1963961..1964479 (-) 519 WP_010464421.1 shikimate kinase AroK -
  PFAS1_RS09125 (PFAS1_09115) pilQ 1964484..1966574 (-) 2091 WP_076028936.1 type IV pilus secretin PilQ Machinery gene
  PFAS1_RS09130 (PFAS1_09120) - 1966588..1967115 (-) 528 WP_076028937.1 pilus assembly protein PilP -
  PFAS1_RS09135 (PFAS1_09125) pilO 1967112..1967735 (-) 624 WP_076028938.1 type 4a pilus biogenesis protein PilO -
  PFAS1_RS09140 (PFAS1_09130) - 1967732..1968298 (-) 567 WP_076028939.1 PilN domain-containing protein -
  PFAS1_RS09145 (PFAS1_09135) comM 1968298..1969362 (-) 1065 WP_076028940.1 pilus assembly protein PilM Machinery gene
  PFAS1_RS09150 (PFAS1_09140) - 1969581..1972022 (+) 2442 WP_371916910.1 penicillin-binding protein 1A -
  PFAS1_RS09155 (PFAS1_09145) - 1972254..1973522 (-) 1269 WP_008149557.1 malic enzyme-like NAD(P)-binding protein -

Sequence

Protein


Download         Length: 354 a.a.        Molecular weight: 38308.84 Da        Isoelectric Point: 4.8154

>NTDB_id=208025 PFAS1_RS09145 WP_076028940.1 1968298..1969362(-) (comM) [Pseudomonas frederiksbergensis strain AS1]
MLGLFNKKANTLLGIDISSTSVKLLELSRQGDRYRVEAYAVEPLPTNAVVEKNIAELEGVGQALARVLLKAKTNLRNVAV
AVAGSAVITKTIEMDAGLSDDEMENQLKIEADQYIPYPLDEVAIDFEVQGVSARNPERVNVLLAACRKENVEVREAALAL
AGLTARVVDVEAYALERSYGLLARQLAASQERFTVAVVDIGATMTTLSVLHQGRIIYTREQLFGGRQLTEEIQRRYGLTI
EQAGLAKKQGGLPDDYVIEVLQPFRDALVQQVSRSLQFFFASGQYSSVDHILLAGGTASVPGLDRLIEQRLGTPTQVANP
FSDMTLSSKVNAGALASDAPALMIACGLALRSFD

Nucleotide


Download         Length: 1065 bp        

>NTDB_id=208025 PFAS1_RS09145 WP_076028940.1 1968298..1969362(-) (comM) [Pseudomonas frederiksbergensis strain AS1]
GTGCTAGGACTCTTCAATAAGAAAGCCAATACGCTTCTGGGGATCGACATCAGCTCCACGTCGGTCAAGCTTCTGGAGCT
AAGCCGCCAAGGCGACCGTTATCGGGTCGAGGCCTATGCGGTGGAACCTTTACCCACTAATGCGGTGGTCGAGAAAAATA
TCGCCGAGCTCGAAGGCGTAGGGCAGGCGCTCGCCCGCGTGTTGCTCAAGGCCAAGACCAACCTCAGGAACGTCGCCGTG
GCCGTGGCCGGTTCGGCGGTGATTACCAAGACCATCGAGATGGACGCCGGTCTTTCCGACGACGAAATGGAAAACCAGCT
GAAGATCGAGGCCGATCAATACATCCCTTATCCATTGGACGAAGTGGCCATCGACTTTGAGGTCCAGGGCGTTTCTGCGC
GCAACCCCGAGCGGGTCAACGTGCTGCTGGCGGCCTGTCGCAAGGAAAACGTCGAGGTCCGGGAAGCGGCCCTGGCCCTG
GCTGGCCTGACAGCTCGCGTGGTCGACGTGGAGGCCTATGCGCTGGAGCGTTCCTACGGATTGCTGGCGAGACAGCTGGC
TGCTTCTCAGGAGCGTTTCACGGTAGCGGTGGTCGATATCGGCGCCACCATGACCACCCTGAGCGTGCTGCACCAGGGGC
GGATCATCTACACCCGCGAGCAATTGTTCGGCGGTCGTCAACTGACGGAAGAAATCCAGCGACGCTATGGCCTGACCATC
GAGCAGGCGGGGCTGGCGAAGAAACAGGGTGGTTTGCCGGACGATTACGTCATTGAGGTGTTACAGCCGTTTCGCGATGC
GCTGGTGCAACAGGTGTCGCGTTCGTTGCAGTTTTTCTTCGCCTCCGGTCAGTACAGCTCGGTCGATCACATTCTGTTGG
CCGGCGGTACCGCATCGGTCCCGGGGCTGGACCGCTTGATCGAGCAACGATTGGGTACGCCGACGCAAGTGGCCAACCCC
TTTTCCGACATGACACTGAGCAGCAAGGTCAACGCCGGTGCCTTGGCCAGCGATGCGCCGGCACTGATGATCGCCTGCGG
GCTGGCCCTCAGGAGTTTCGACTGA

Domains

Predicted by InterproScan.

Type IV pilus assembly protein PilM; (PilM_2)

(14-353)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  comM Acinetobacter nosocomialis M2

56.78

100

0.568
  pilM Acinetobacter baumannii D1279779

56.497

100

0.565
  comM Acinetobacter baylyi ADP1

54.237

100

0.542
  pilM Legionella pneumophila strain ERS1305867

48.588

100

0.486

Multiple sequence alignment