Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilB   Type   Machinery gene
Locus tag   A2U20_RS07325 Genome accession   NZ_CP018032
Coordinates   1529389..1530774 (+) Length   461 a.a.
NCBI ID   WP_021117771.1    Uniprot ID   -
Organism   Glaesserella parasuis D74     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 1524389..1535774
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  A2U20_RS07305 (A2U20_07195) gpt 1525717..1526196 (+) 480 WP_005714020.1 xanthine phosphoribosyltransferase -
  A2U20_RS07310 (A2U20_07200) gmk 1526450..1527076 (+) 627 WP_021117774.1 guanylate kinase -
  A2U20_RS07315 (A2U20_07205) radA 1527129..1528505 (-) 1377 WP_021117773.1 DNA repair protein RadA -
  A2U20_RS07320 (A2U20_07210) pilA 1528693..1529148 (+) 456 WP_021117772.1 prepilin-type N-terminal cleavage/methylation domain-containing protein Machinery gene
  A2U20_RS07325 (A2U20_07215) pilB 1529389..1530774 (+) 1386 WP_021117771.1 GspE/PulE family protein Machinery gene
  A2U20_RS07330 (A2U20_07220) pilC 1530767..1531963 (+) 1197 WP_021117770.1 type II secretion system F family protein Machinery gene
  A2U20_RS07335 (A2U20_07225) - 1531960..1532628 (+) 669 WP_021117769.1 prepilin peptidase -
  A2U20_RS07340 (A2U20_07230) dapE 1532712..1533845 (+) 1134 WP_021117768.1 succinyl-diaminopimelate desuccinylase -
  A2U20_RS07345 (A2U20_07235) clpX 1533919..1535169 (-) 1251 WP_021117767.1 ATP-dependent protease ATP-binding subunit ClpX -
  A2U20_RS07350 (A2U20_07240) clpP 1535176..1535757 (-) 582 WP_010787003.1 ATP-dependent Clp endopeptidase proteolytic subunit ClpP -

Sequence

Protein


Download         Length: 461 a.a.        Molecular weight: 52704.48 Da        Isoelectric Point: 4.9814

>NTDB_id=205184 A2U20_RS07325 WP_021117771.1 1529389..1530774(+) (pilB) [Glaesserella parasuis D74]
MSQYTVCEQQTERIFDISPSRWQQNCEEKELLLRYLAVPVQETEHKLWLAVDDENNLTACEIFAFMTHKQIEPVVIASDE
LKYLLNALSPEQQPLYEESELAFVEQEQEQLNLSDPIIQLLDNLFKFCLAQNASDIHIEPRKQKLIIRLRIDGVLHLYKS
LSIQLASRLISRIKLLAKLDISELRQPQDGQFSFTTALADTLDFRVSSLPTIYGEKLVLRLQKNKPTSFDFLQLGFNPQQ
QTTLLNALKQPQGLILVTGPTGSGKSITLYSALNYLNQMDKHILTAEDPVEIEIDGLIQTQVNQSINLDFSQLLRTFLRQ
DPDIIMLGEIRDEESAKMALRASQTGHLVLSTLHTNDALLAVERLLQLGIQEYEIQNSLLLVIAQRLVRKLCKKCRGTGC
DECYQGYKGRIGIYQCLSRTAKKFDKQTAYLDFSSLRESAKQKIEQQLTNEIEVDRVLGDE

Nucleotide


Download         Length: 1386 bp        

>NTDB_id=205184 A2U20_RS07325 WP_021117771.1 1529389..1530774(+) (pilB) [Glaesserella parasuis D74]
ATGTCCCAATACACCGTCTGCGAACAACAAACCGAACGCATTTTTGATATATCGCCTAGCCGTTGGCAACAAAATTGCGA
AGAAAAAGAGCTGTTATTGCGTTATCTTGCCGTTCCTGTGCAAGAAACTGAGCATAAATTATGGCTTGCCGTTGATGATG
AAAACAATCTCACCGCTTGCGAAATCTTTGCTTTTATGACCCATAAGCAGATTGAACCTGTGGTAATTGCTTCTGATGAG
CTTAAATACTTGCTTAACGCACTCTCGCCTGAGCAACAACCCCTTTATGAAGAGAGCGAACTGGCTTTCGTTGAACAAGA
GCAAGAACAGCTCAACTTAAGCGATCCGATTATTCAACTGCTCGATAATCTTTTTAAATTCTGTTTAGCCCAAAATGCGT
CCGACATTCATATTGAACCACGCAAACAAAAATTGATTATTCGTCTACGCATTGACGGTGTTTTGCACCTGTATAAAAGC
CTGTCTATTCAACTGGCAAGCCGACTTATTTCACGGATAAAACTGCTGGCGAAGCTAGATATTAGTGAATTACGCCAGCC
ACAAGATGGGCAATTTAGCTTTACTACCGCCTTGGCGGACACCCTTGATTTTCGGGTATCTAGCCTGCCGACGATCTACG
GCGAAAAACTGGTATTGCGCCTACAAAAAAACAAACCAACCAGTTTTGATTTTCTGCAACTTGGCTTTAATCCACAACAG
CAGACCACACTACTCAACGCCTTGAAACAACCGCAAGGGCTGATTTTAGTGACAGGACCAACGGGCAGTGGCAAAAGTAT
CACCTTGTATAGTGCCTTAAACTACCTCAACCAAATGGATAAACATATTCTCACCGCCGAAGATCCTGTGGAAATTGAGA
TTGACGGCTTAATTCAAACCCAAGTCAATCAAAGTATTAACCTTGATTTTAGCCAGCTATTACGCACCTTTCTACGCCAA
GATCCCGACATCATTATGCTAGGTGAAATTCGAGATGAAGAGAGTGCAAAAATGGCATTAAGAGCAAGCCAGACGGGGCA
TTTAGTCCTTTCAACCCTGCATACCAATGACGCACTATTAGCGGTCGAACGTTTGTTACAACTGGGTATTCAAGAGTATG
AAATCCAAAATTCACTACTGTTAGTCATTGCACAACGCCTTGTGCGTAAGCTCTGCAAAAAGTGTCGTGGCACAGGCTGT
GATGAGTGCTATCAAGGCTATAAAGGGCGGATCGGGATTTACCAATGTTTAAGTAGAACTGCAAAAAAATTCGATAAACA
GACCGCTTACTTAGATTTTTCCAGCTTACGAGAGAGTGCTAAACAAAAAATTGAACAACAACTAACCAACGAAATCGAAG
TGGATAGGGTATTAGGCGATGAATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein E, N-terminal domain (MshEN)

(24-92)

Type II/IV secretion system protein (T2SSE)

(129-390)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilB Glaesserella parasuis strain SC1401

97.18

100

0.972
  pilB Haemophilus influenzae 86-028NP

57.174

99.783

0.57
  pilB Haemophilus influenzae Rd KW20

57.802

98.698

0.57
  pilB Vibrio cholerae strain A1552

38.755

100

0.419
  pilB Vibrio parahaemolyticus RIMD 2210633

39.709

100

0.414
  pilB Vibrio campbellii strain DS40M4

39.293

100

0.41
  pilB Acinetobacter baylyi ADP1

36.634

100

0.401
  pilB Acinetobacter baumannii D1279779

35.992

100

0.401
  pilB Deinococcus radiodurans R1 = ATCC 13939 = DSM 20539

38.889

100

0.395
  pilB Legionella pneumophila strain ERS1305867

40.682

95.445

0.388
  pilF Neisseria gonorrhoeae MS11

36.402

100

0.377

Multiple sequence alignment