Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   BM525_RS13670 Genome accession   NZ_CP018027
Coordinates   3158005..3159213 (+) Length   402 a.a.
NCBI ID   WP_071952412.1    Uniprot ID   -
Organism   Alteromonas mediterranea strain CP49     
Function   assembly of type IV pilus (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 3153005..3164213
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  BM525_RS13645 (BM525_13570) ampE 3153970..3154839 (-) 870 WP_015067808.1 beta-lactamase regulator AmpE -
  BM525_RS13650 (BM525_13575) ampD 3154870..3155406 (-) 537 WP_071952404.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  BM525_RS13655 (BM525_13580) - 3155582..3156100 (+) 519 WP_071952406.1 retropepsin-like aspartic protease family protein -
  BM525_RS13660 (BM525_13585) nadC 3156120..3156974 (+) 855 WP_071952408.1 carboxylating nicotinate-nucleotide diphosphorylase -
  BM525_RS13665 (BM525_13590) - 3157510..3157932 (+) 423 WP_071952410.1 pilin -
  BM525_RS13670 (BM525_13595) pilC 3158005..3159213 (+) 1209 WP_071952412.1 type II secretion system F family protein Machinery gene
  BM525_RS13675 (BM525_13600) pilD 3159331..3160224 (+) 894 WP_071952414.1 prepilin peptidase Machinery gene
  BM525_RS13680 (BM525_13605) coaE 3160246..3160860 (+) 615 WP_071953272.1 dephospho-CoA kinase -
  BM525_RS13685 (BM525_13610) zapD 3161074..3161826 (+) 753 WP_015067815.1 cell division protein ZapD -
  BM525_RS13690 (BM525_13615) yacG 3161917..3162144 (+) 228 WP_012519434.1 DNA gyrase inhibitor YacG -
  BM525_RS13695 (BM525_13620) - 3162337..3163209 (+) 873 WP_071952416.1 aspartoacylase -
  BM525_RS13700 (BM525_13625) mutT 3163300..3163683 (-) 384 WP_071952418.1 8-oxo-dGTP diphosphatase MutT -

Sequence

Protein


Download         Length: 402 a.a.        Molecular weight: 43670.33 Da        Isoelectric Point: 9.6771

>NTDB_id=205129 BM525_RS13670 WP_071952412.1 3158005..3159213(+) (pilC) [Alteromonas mediterranea strain CP49]
MAKAALTFTWQGKDRNGQARKGEISATSLSEAKNLLRRQGISANKVKKLAKPLFGGAKKITPADISVISRQIATMLAAGV
TLIQSLDMIAQGHANPSVRKLLGEITEEVKSGNPLSSALRKHPLYFDDLYCDLVYTGEQSGALETIYDRIATYKEKAEAL
KSKIKKAMFYPIAVLVVAFIVTTILLIFVVPQFEEIFSSFGAELPAFTQFVLAISRFVQDYGIFIAMGFAATGFMFVRAH
RRSQKLRDSVDRNILKIPVIGEILKKASIARFTRTLATTFAAGVPLIGALESAAGASGNAVYRDAILYIRKEVAGGMPMY
VAMRATQVFPDMVTQMIAIGEESGSVDEMLSKIATIYEAEVDDMVDGLTSLLEPMIMAVLGVVIGGLIVAMYLPIFQMGN
VV

Nucleotide


Download         Length: 1209 bp        

>NTDB_id=205129 BM525_RS13670 WP_071952412.1 3158005..3159213(+) (pilC) [Alteromonas mediterranea strain CP49]
ATGGCAAAAGCAGCTCTCACATTTACATGGCAAGGTAAAGACCGTAACGGTCAAGCTCGAAAAGGCGAAATATCGGCTAC
CTCGCTTTCTGAAGCAAAGAACCTTTTGCGTCGCCAAGGTATCTCTGCAAACAAAGTCAAAAAGCTTGCCAAACCACTTT
TCGGTGGCGCAAAGAAAATTACGCCAGCAGATATCTCGGTAATATCTCGTCAAATCGCGACTATGCTTGCCGCGGGTGTT
ACGCTAATTCAGTCACTAGATATGATAGCGCAAGGTCATGCAAACCCTTCGGTGCGTAAACTGCTTGGTGAAATTACTGA
AGAAGTCAAATCAGGTAATCCACTGTCTTCTGCTCTCAGAAAACACCCCCTTTATTTTGACGATTTGTACTGTGACTTGG
TTTACACAGGCGAGCAATCGGGAGCCCTTGAAACTATTTACGACCGAATTGCTACTTATAAAGAAAAAGCGGAGGCGCTA
AAATCAAAGATCAAAAAAGCGATGTTTTATCCTATCGCCGTTTTGGTGGTTGCTTTTATTGTTACTACTATCCTTCTTAT
TTTCGTTGTGCCTCAGTTTGAAGAAATCTTTAGCAGCTTTGGCGCTGAACTCCCTGCGTTTACGCAGTTCGTACTGGCTA
TATCGCGGTTTGTACAGGATTACGGTATTTTTATCGCCATGGGTTTTGCTGCGACAGGATTTATGTTTGTGCGTGCACAC
AGACGTAGCCAAAAGCTGCGCGATAGCGTAGACCGTAATATATTAAAAATACCTGTTATCGGCGAGATACTTAAAAAAGC
CAGTATCGCCCGCTTTACCCGAACCCTTGCCACCACCTTTGCGGCAGGTGTACCGCTAATAGGCGCGTTAGAGTCGGCAG
CGGGTGCATCGGGTAACGCTGTTTATCGAGACGCGATACTATATATACGCAAAGAAGTGGCTGGCGGTATGCCAATGTAC
GTTGCCATGCGCGCAACCCAGGTATTTCCAGACATGGTGACGCAAATGATAGCCATAGGTGAAGAGTCTGGCTCTGTCGA
TGAGATGCTAAGTAAAATTGCCACCATTTACGAAGCAGAAGTTGATGACATGGTAGACGGCTTAACCAGCTTGCTTGAGC
CTATGATCATGGCCGTACTTGGCGTGGTTATTGGCGGCTTGATTGTGGCCATGTACCTGCCTATATTCCAGATGGGTAAC
GTGGTATAA

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(272-394)

Type II secretion system (T2SS), protein F (T2SSF)

(69-191)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Pseudomonas stutzeri DSM 10701

58.765

100

0.592
  pilC Legionella pneumophila strain ERS1305867

53.5

99.502

0.532
  pilC Acinetobacter baumannii D1279779

52.764

99.005

0.522
  pilC Acinetobacter baylyi ADP1

52.141

98.756

0.515
  pilG Neisseria gonorrhoeae MS11

44.802

100

0.45
  pilG Neisseria meningitidis 44/76-A

44.802

100

0.45
  pilC Vibrio cholerae strain A1552

45.34

98.756

0.448
  pilC Vibrio campbellii strain DS40M4

44

99.502

0.438
  pilC Thermus thermophilus HB27

36.75

99.502

0.366

Multiple sequence alignment