Detailed information    

insolico Bioinformatically predicted

Overview

Name   pilC   Type   Machinery gene
Locus tag   K01M1_RS13515 Genome accession   NZ_CP017889
Coordinates   2851246..2852469 (+) Length   407 a.a.
NCBI ID   WP_054730505.1    Uniprot ID   A0A1W6UNK4
Organism   Vibrio alginolyticus strain K01M1     
Function   type IV pilus biogenesis and function (predicted from homology)   
DNA binding and uptake

Genomic Context

Location: 2846246..2857469
Locus tag Gene name Coordinates (strand) Size (bp) Protein ID Product Description
  K01M1_RS13495 (K01M1_26090) ampD 2847275..2847826 (-) 552 WP_042521337.1 1,6-anhydro-N-acetylmuramyl-L-alanine amidase AmpD -
  K01M1_RS13500 (K01M1_26100) nadC 2847919..2848806 (+) 888 WP_005379983.1 carboxylating nicotinate-nucleotide diphosphorylase -
  K01M1_RS13505 (K01M1_26110) pilA 2849069..2849533 (+) 465 WP_033905592.1 pilin Machinery gene
  K01M1_RS13510 (K01M1_26120) pilB 2849537..2851222 (+) 1686 WP_054730502.1 type IV-A pilus assembly ATPase PilB Machinery gene
  K01M1_RS13515 (K01M1_26130) pilC 2851246..2852469 (+) 1224 WP_054730505.1 type II secretion system F family protein Machinery gene
  K01M1_RS13520 (K01M1_26140) pilD 2852534..2853403 (+) 870 WP_086047031.1 prepilin peptidase Machinery gene
  K01M1_RS13525 (K01M1_26150) coaE 2853404..2854018 (+) 615 WP_017635920.1 dephospho-CoA kinase -
  K01M1_RS13530 (K01M1_26160) zapD 2854046..2854786 (+) 741 WP_005373086.1 cell division protein ZapD -
  K01M1_RS13535 (K01M1_26170) yacG 2854945..2855139 (+) 195 WP_017635922.1 DNA gyrase inhibitor YacG -
  K01M1_RS13540 (K01M1_26180) rplS 2855794..2856147 (-) 354 WP_005379971.1 50S ribosomal protein L19 -
  K01M1_RS13545 (K01M1_26190) trmD 2856189..2856950 (-) 762 WP_005385420.1 tRNA (guanosine(37)-N1)-methyltransferase TrmD -

Sequence

Protein


Download         Length: 407 a.a.        Molecular weight: 45285.44 Da        Isoelectric Point: 10.3992

>NTDB_id=203662 K01M1_RS13515 WP_054730505.1 2851246..2852469(+) (pilC) [Vibrio alginolyticus strain K01M1]
MKSTTPQLKNFRWKGINSSGKKTSGQTLAMTEIEVRERLDAQHIKIKKLKKSSISFLTKLSHRVKGRDITVFTRQISTML
ITGVPLAQALKLVSENHKKAEMKSILMSVTRAVEAGTPMSKAMRTASEHFDPLYTDLIATGEQSGNLAEVFERLATYREK
NEQLRAKVIKALIYPAMVVLVALGVSFIMLTKVIPEFEKMFVGFGADLPWFTRQVLDLSAWTQNWSPFIALGSISLFISA
RILSKRSDSFRLMLNRSVLKFPVLGPVLSKAAIAKFSRTLATSFTAGIPILTSLKTTSKTSGNLHYQLAIEEVYRDTAAG
MPMYVAMRNCNVFPELVLQMVMIGEESGRLDDMLNKVATIYEFEVDNTVDNLSKILEPLIIVFLGIVVGGLVTAMYLPIF
NLMSVLG

Nucleotide


Download         Length: 1224 bp        

>NTDB_id=203662 K01M1_RS13515 WP_054730505.1 2851246..2852469(+) (pilC) [Vibrio alginolyticus strain K01M1]
ATGAAAAGCACTACACCACAACTTAAAAACTTCCGCTGGAAAGGCATCAACAGTTCTGGCAAAAAGACGTCTGGACAAAC
CCTCGCGATGACAGAAATTGAAGTACGCGAGCGTCTAGACGCACAGCACATCAAGATCAAGAAGTTAAAGAAAAGCAGTA
TTTCGTTTCTCACTAAACTCAGCCATCGCGTAAAAGGCAGAGACATCACGGTGTTTACCCGCCAAATTTCGACGATGTTG
ATAACCGGTGTGCCCTTGGCTCAGGCTTTAAAATTGGTATCAGAGAATCACAAAAAAGCAGAGATGAAATCCATTTTGAT
GAGCGTGACACGCGCCGTTGAAGCGGGGACGCCCATGTCAAAAGCCATGCGCACTGCAAGTGAACACTTTGACCCACTCT
ATACTGATCTTATCGCCACAGGTGAGCAATCCGGTAACTTAGCGGAAGTGTTCGAGCGCTTAGCCACCTACCGAGAAAAA
AATGAACAACTCCGTGCGAAAGTGATCAAAGCACTGATTTACCCAGCGATGGTAGTGCTAGTAGCGTTAGGCGTGTCGTT
TATCATGCTCACCAAAGTCATTCCCGAGTTCGAAAAAATGTTTGTTGGTTTTGGTGCTGACTTACCTTGGTTTACCAGGC
AAGTGTTAGATCTTTCCGCCTGGACACAAAACTGGAGTCCGTTTATCGCACTAGGCTCCATTAGTTTATTCATTTCGGCT
AGAATCCTCTCTAAGCGTTCAGATTCTTTTCGCTTAATGCTCAACCGCTCTGTGCTTAAATTTCCTGTCCTTGGGCCTGT
ATTATCAAAAGCCGCTATCGCCAAATTTAGTCGAACACTCGCCACAAGCTTTACAGCGGGTATTCCAATTCTAACCAGTT
TAAAAACCACATCAAAAACCTCAGGAAACTTACACTATCAACTCGCCATAGAAGAAGTTTACCGTGATACCGCCGCAGGT
ATGCCAATGTATGTTGCCATGCGTAACTGTAATGTGTTCCCTGAATTAGTGTTACAAATGGTCATGATCGGTGAAGAGTC
TGGTCGACTTGACGATATGCTCAATAAAGTTGCAACGATCTACGAGTTTGAAGTCGACAACACCGTCGACAACCTTAGTA
AAATTCTAGAGCCACTAATTATCGTATTTTTAGGTATCGTTGTTGGCGGATTAGTGACAGCAATGTACTTGCCAATCTTT
AACTTAATGAGTGTATTGGGCTAG

Domains

Predicted by InterproScan.

Type II secretion system (T2SS), protein F (T2SSF)

(276-398)

Type II secretion system (T2SS), protein F (T2SSF)

(72-195)


Secondary structure

Protein secondary structures were predicted by S4PRED and visualized by seqviz.



3D structure

Source ID Structure
  AlphaFold DB A0A1W6UNK4

Transmembrane helices

Transmembrane helices of protein were predicted by TMHMM 2.0 and visualized by seqviz and ECharts.


Visualization of predicted probability:


Similar proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
  pilC Vibrio campbellii strain DS40M4

86.978

100

0.87
  pilC Vibrio cholerae strain A1552

73.529

100

0.737
  pilC Acinetobacter baylyi ADP1

42.75

98.28

0.42
  pilC Acinetobacter baumannii D1279779

41.191

99.017

0.408
  pilG Neisseria gonorrhoeae MS11

40.494

99.509

0.403
  pilG Neisseria meningitidis 44/76-A

40.247

99.509

0.4
  pilC Legionella pneumophila strain ERS1305867

40.049

100

0.4
  pilC Pseudomonas stutzeri DSM 10701

40.909

97.297

0.398

Multiple sequence alignment