Detailed information NTG0203663

Bioinformatically predicted

Overview

• Name: pilD
• Type: Machinery gene
• Locus tag: K01M1_RS13520
• Genome accession: NZ_CP017889
• Coordinates: 2852534..2853403 (+)
• Length: 289 a.a.
• NCBI ID: WP_086047031.1
• Uniprot ID: A0A1W6TUE5
• Organism: Vibrio alginolyticus strain K01M1
• Function: type IV pilus biogenesis and function (predicted from homology) DNA binding and uptake

Genomic Context

Location: 2847534..2858403

Locus tag	Gene name	Coordinates (strand)	Size (bp)	Protein ID	Product	Description
K01M1_RS13500 (K01M1_26100)	nadC	2847919..2848806 (+)	888	WP_005379983.1	carboxylating nicotinate-nucleotide diphosphorylase	-
K01M1_RS13505 (K01M1_26110)	pilA	2849069..2849533 (+)	465	WP_033905592.1	pilin	Machinery gene
K01M1_RS13510 (K01M1_26120)	pilB	2849537..2851222 (+)	1686	WP_054730502.1	type IV-A pilus assembly ATPase PilB	Machinery gene
K01M1_RS13515 (K01M1_26130)	pilC	2851246..2852469 (+)	1224	WP_054730505.1	type II secretion system F family protein	Machinery gene
K01M1_RS13520 (K01M1_26140)	pilD	2852534..2853403 (+)	870	WP_086047031.1	prepilin peptidase	Machinery gene
K01M1_RS13525 (K01M1_26150)	coaE	2853404..2854018 (+)	615	WP_017635920.1	dephospho-CoA kinase	-
K01M1_RS13530 (K01M1_26160)	zapD	2854046..2854786 (+)	741	WP_005373086.1	cell division protein ZapD	-
K01M1_RS13535 (K01M1_26170)	yacG	2854945..2855139 (+)	195	WP_017635922.1	DNA gyrase inhibitor YacG	-
K01M1_RS13540 (K01M1_26180)	rplS	2855794..2856147 (-)	354	WP_005379971.1	50S ribosomal protein L19	-
K01M1_RS13545 (K01M1_26190)	trmD	2856189..2856950 (-)	762	WP_005385420.1	tRNA (guanosine(37)-N1)-methyltransferase TrmD	-
K01M1_RS13550 (K01M1_26200)	rimM	2856978..2857526 (-)	549	WP_005379964.1	ribosome maturation factor RimM	-
K01M1_RS13555 (K01M1_26210)	rpsP	2857556..2857804 (-)	249	WP_005379962.1	30S ribosomal protein S16	-

Sequence

Protein

• Length: 289 a.a.
• Molecular weight: 32547.76 Da
• Isoelectric Point: 6.8455

>NTDB_id=203663 K01M1_RS13520 WP_086047031.1 2852534..2853403(+) (pilD) [Vibrio alginolyticus strain K01M1]
MEVFQYYPWLFVVFATIFGLIVGSFLNVVIYRLPKIMELEWRRECAESFPEYKIEPPKEVLTLSVPRSSCQKCATPIRIR
DNIPVISWLLLKGKCHNCQATISARYPLIELLTAFCSGFIAYYFGFSYFTVALLFFTFILIAATFIDLDTMLLPDQLTLP
LTWAGIALALVEISPVALQDSVIGAIAGYLCLWSVYWGFKLLTGKEGMGYGDFKLLAALGAWLGWQSLPMIILLSSVVGI
VFGIIQLRLQKQGIEKAFPFGPYLAIAGWISLIWGEQILNWYFTSVLGV

Nucleotide

• Length: 870 bp

>NTDB_id=203663 K01M1_RS13520 WP_086047031.1 2852534..2853403(+) (pilD) [Vibrio alginolyticus strain K01M1]
ATGGAAGTATTCCAGTACTACCCTTGGCTATTCGTTGTGTTTGCCACCATCTTCGGTCTGATCGTGGGTAGCTTCCTCAA
TGTGGTTATTTATCGACTACCTAAAATCATGGAACTAGAATGGCGACGCGAATGTGCCGAGTCATTTCCTGAATACAAAA
TAGAACCTCCTAAAGAAGTGCTGACCTTGAGCGTCCCACGCTCCTCCTGCCAGAAATGCGCAACACCGATACGTATTCGC
GACAACATTCCTGTGATTAGCTGGCTGCTTTTAAAAGGCAAATGTCACAACTGTCAGGCAACCATTAGTGCACGCTATCC
ACTGATTGAACTGCTCACCGCGTTCTGTTCAGGCTTTATAGCTTATTACTTTGGTTTTAGTTACTTTACCGTTGCCCTAC
TCTTTTTTACTTTTATCTTAATCGCCGCAACATTTATCGATCTCGATACTATGTTGTTACCTGACCAATTAACATTACCA
TTAACTTGGGCTGGCATTGCCTTAGCATTAGTCGAAATAAGTCCTGTTGCTCTACAAGATTCTGTCATTGGTGCTATTGC
TGGTTACCTATGTCTCTGGTCCGTTTACTGGGGCTTTAAGCTACTTACTGGTAAAGAAGGAATGGGATATGGTGATTTTA
AACTACTAGCAGCACTAGGGGCTTGGCTAGGTTGGCAGTCACTGCCGATGATCATTCTACTTTCTTCTGTGGTTGGCATC
GTATTTGGCATCATTCAACTACGCTTGCAAAAGCAGGGAATTGAAAAAGCTTTCCCTTTCGGTCCTTACTTAGCAATTGC
TGGTTGGATAAGCTTAATTTGGGGCGAGCAGATTTTAAACTGGTACTTTACTTCAGTTCTAGGAGTGTAA

Domains

Predicted by InterproScan.

• Bacterial Peptidase A24 N-terminal domain (A24_N_bact) (17-124)

• Type IV leader peptidase family (Peptidase_A24) (135-244)

3D structure

• AlphaFold DB: A0A1W6TUE5

Similar proteins

Only experimentally validated proteins are listed.

Protein	Organism	Identities (%)	Coverage (%)	Ha-value
pilD	Vibrio campbellii strain DS40M4	91.349	100	0.913
pilD	Vibrio cholerae strain A1552	72.917	99.654	0.727
pilD	Acinetobacter baumannii D1279779	51.852	93.426	0.484
pilD	Acinetobacter nosocomialis M2	51.481	93.426	0.481
pilD	Neisseria gonorrhoeae MS11	47.464	95.502	0.453